Results 41 to 50 of about 902,297 (302)
High-throughput total RNA sequencing in single cells using VASA-seq
Nature Biotechnology, 2022 Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome.
Fredrik Salmén +15 more
semanticscholar +1 more source
Ion torrent high throughput mitochondrial genome sequencing (HTMGS).
PLoS ONE, 2019 The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in
N R Harvey +10 more
doaj +1 more source
First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]
Scientia Agricola, 2023 Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara +8 more
doaj +1 more source
Applications in Plant Sciences, 2016 Premise of the study: Using sequence data generated via target enrichment for phylogenetics requires reassembly of high-throughput sequence reads into loci, presenting a number of bioinformatics challenges.
Matthew G. Johnson +7 more
semanticscholar +1 more source
Targeted high throughput sequencing in hereditary ataxia and spastic paraplegia. [PDF]
PLoS ONE, 2017 Hereditary ataxia and spastic paraplegia are heterogeneous monogenic neurodegenerative disorders. To date, a large number of individuals with such disorders remain undiagnosed.
Zafar Iqbal +8 more
doaj +1 more source
Perspectives and Benefits of High-Throughput Long-Read Sequencing in Microbial Ecology
Applied and Environmental Microbiology, 2021 Short-read, high-throughput sequencing (HTS) methods have yielded numerous important insights into microbial ecology and function. Yet, in many instances short-read HTS techniques are suboptimal, for example, by providing insufficient phylogenetic ...
L. Tedersoo +3 more
semanticscholar +1 more source
Optimal assembly for high throughput shotgun sequencing [PDF]
BMC Bioinformatics, 2013 We present a framework for the design of optimal assembly algorithms for shotgun sequencing under the criterion of complete reconstruction. We derive a lower bound on the read length and the coverage depth required for reconstruction in terms of the repeat statistics of the genome.
Bresler, Guy +2 more
openaire +5 more sources
High Throughput Sequencing for the Detection and Characterization of RNA Viruses
Frontiers in Microbiology, 2021 This review aims to assess and recommend approaches for targeted and agnostic High Throughput Sequencing of RNA viruses in a variety of sample matrices.
Amy H. Fitzpatrick +5 more
semanticscholar +1 more source
High-Throughput Sequencing and Rare Genetic Diseases [PDF]
Molecular Syndromology, 2012 High-throughput sequencing has drastically changed the research of genes responsible for genetic disorders and is now gradually introduced as an additional genetic diagnostic testing in clinical practice. The current debates on the emerging technical, medical and ethical issues as well as the potential optimum use of the available technology are ...
Makrythanasis P, Antonarakis SE.
openaire +3 more sources
Nature Communications, 2023 MicroRNAs (miRNAs) regulate fundamental biological processes by silencing mRNA targets and are dysregulated in many diseases. Therefore, miRNA replacement or inhibition can be harnessed as potential therapeutics.
Lien D. Nguyen +11 more
doaj +1 more source