Results 21 to 30 of about 16,449,464 (307)

Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples. [PDF]

open access: yes, 2012
peer-reviewedBackground: The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an ...
Magee, David A   +52 more
core   +1 more source

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]

open access: yes, 2014
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A   +83 more
core   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

open access: yes, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J.   +27 more
core   +2 more sources

High throughput error corrected Nanopore single cell transcriptome sequencing

open access: yesNature Communications, 2020
Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full
Kevin Lebrigand   +3 more
doaj   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

open access: yes, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P.   +50 more
core   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

open access: yesAnimals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul   +6 more
doaj   +1 more source

High-Throughput Microdissection for Next-Generation Sequencing. [PDF]

open access: yesPLoS ONE, 2016
Precision medicine promises to enhance patient treatment through the use of emerging molecular technologies, including genomics, transcriptomics, and proteomics.
Avi Z Rosenberg   +17 more
doaj   +1 more source

First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]

open access: yesScientia Agricola, 2023
Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara   +8 more
doaj   +1 more source

DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]

open access: yes, 2011
The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Michael Bunce   +38 more
core   +1 more source

High Throughput Sequencing for Detection of Foodborne Pathogens

open access: yesFrontiers in Microbiology, 2017
High-throughput sequencing (HTS) is becoming the state-of-the-art technology for typing of microbial isolates, especially in clinical samples. Yet, its application is still in its infancy for monitoring and outbreak investigations of foods.
Camilla Sekse   +6 more
doaj   +1 more source

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