Base calling for high-throughput short-read sequencing: dynamic programming solutions [PDF]
, 2013 Shreepriya Das and Haris Vikalo are with the Electrical and Computer Engineering Department, The University of Texas at Austin, Austin, Texas 78712, USABackground: Next-generation DNA sequencing platforms are capable of generating millions of reads in ...
Das, Shreepriya, Vikalo, Haris
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High Throughput Sequencing for Detection of Foodborne Pathogens
Frontiers in Microbiology, 2017 High-throughput sequencing (HTS) is becoming the state-of-the-art technology for typing of microbial isolates, especially in clinical samples. Yet, its application is still in its infancy for monitoring and outbreak investigations of foods.
Camilla Sekse +6 more
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Effects of propidium monoazide (PMA) treatment on mycobiome and bacteriome analysis of cystic fibrosis airways during exacerbation [PDF]
, 2016 Introduction and Purpose : Propidium monoazide (PMA)-pretreatment has increasingly been applied to remove the bias from dead or damaged cell artefacts, which could impact the microbiota analysis by high-throughput sequencing. Our study aimed to determine
Audebert, Christophe +8 more
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High‐Throughput Multiplex Sequencing of miRNA [PDF]
Current Protocols in Human Genetics, 2012 AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
Francois, Vigneault +7 more
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Whole-genome sequencing and the clinician: a tale of two cities [PDF]
, 2014 Clinicians are faced with unprecedented opportunities to identify the genetic aetiologies of hitherto molecularly uncharacterised conditions via the use of high-throughput sequencing.
A. R. Foley +21 more
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SnapShot: High-Throughput Sequencing Applications
Cell, 2011 / 25 (X2) 8 daysPacific Biosciences http://www.pacificbiosciences.comNo amplification; Synthesis Fluorescence >1000 bp (SE) 150,000 per SMRT cell N/AIon Torrent http://www.iontorrent.comEmulsion PCR; Synthesis Change in pH 200 bp (SE) Variable 90% of the full genome resolved (both alleles)400 human genomes per monthModified and updated from Metzker ...
Han, Hong +3 more
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Ion torrent high throughput mitochondrial genome sequencing (HTMGS).
PLoS ONE, 2019 The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in
N R Harvey +10 more
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First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]
Scientia Agricola, 2023 Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara +8 more
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Combined aptamer and transcriptome sequencing of single cells. [PDF]
, 2018 The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput ...
Abate, Adam R +3 more
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Targeted high throughput sequencing in hereditary ataxia and spastic paraplegia. [PDF]
PLoS ONE, 2017 Hereditary ataxia and spastic paraplegia are heterogeneous monogenic neurodegenerative disorders. To date, a large number of individuals with such disorders remain undiagnosed.
Zafar Iqbal +8 more
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