Results 21 to 30 of about 15,584,188 (261)

First occurrence of bidens mottle virus in Brazil: biological and molecular characterization of isolates infecting Zinnia sp. and Bidens pilosa [PDF]

open access: yesScientia Agricola, 2023
Zinnia sp. and hairy beggartick (Bidens pilosa) plants exhibiting symptoms of possible virus infection were found in the municipality of Santa Bárbara d’Oeste, São Paulo State, Brazil.
Gabriel Madoglio Favara   +8 more
doaj   +1 more source

DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]

open access: yes, 2011
The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Michael Bunce   +38 more
core   +1 more source

High Throughput Sequencing for Detection of Foodborne Pathogens

open access: yesFrontiers in Microbiology, 2017
High-throughput sequencing (HTS) is becoming the state-of-the-art technology for typing of microbial isolates, especially in clinical samples. Yet, its application is still in its infancy for monitoring and outbreak investigations of foods.
Camilla Sekse   +6 more
doaj   +1 more source

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

open access: yesFrontiers in Microbiology, 2021
Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and ...
Anna Muszewska   +14 more
doaj   +1 more source

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

open access: yes, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan   +23 more
core   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

open access: yes, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P.   +68 more
core   +1 more source

Ion torrent high throughput mitochondrial genome sequencing (HTMGS).

open access: yesPLoS ONE, 2019
The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in
N R Harvey   +10 more
doaj   +1 more source

Targeted high throughput sequencing in hereditary ataxia and spastic paraplegia. [PDF]

open access: yesPLoS ONE, 2017
Hereditary ataxia and spastic paraplegia are heterogeneous monogenic neurodegenerative disorders. To date, a large number of individuals with such disorders remain undiagnosed.
Zafar Iqbal   +8 more
doaj   +1 more source

Spatiotemporal characteristics of environmental microbial communities and resistomes in intensive care units of a tertiary hospital in northwest China

open access: yesFrontiers in Microbiology
BackgroundIntensive care units (ICUs) are high-risk settings for healthcare-acquired infections (HAIs). It is essential to characterize the microorganisms and antimicrobial resistance genes (ARGs) in ICU environments for prevention and control of HAIs ...
Jiexi Yan   +13 more
doaj   +1 more source

Small molecule regulators of microRNAs identified by high-throughput screen coupled with high-throughput sequencing

open access: yesNature Communications, 2023
MicroRNAs (miRNAs) regulate fundamental biological processes by silencing mRNA targets and are dysregulated in many diseases. Therefore, miRNA replacement or inhibition can be harnessed as potential therapeutics.
Lien D. Nguyen   +11 more
doaj   +1 more source

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