Results 21 to 30 of about 902,297 (302)
High-throughput RNA isoform sequencing using programmed cDNA concatenation
Nature Biotechnology, 2023 Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating ...
Aziz M. Al’Khafaji +18 more
semanticscholar +1 more source
High‐throughput sequencing of the melanoma genome [PDF]
Experimental Dermatology, 2012 AbstractNext‐generation sequencing technologies are now common for whole‐genome, whole‐exome and whole‐transcriptome sequencing (RNA‐seq) of tumors to identify point mutations, structural or copy number alterations and changes in gene expression. A substantial number of studies have already been performed for melanoma. One study analysed eight melanoma
Manfred, Kunz +2 more
openaire +2 more sources
High Throughput Sequencing of Extracellular RNA from Human Plasma. [PDF]
PLoS ONE, 2017 The presence and relative stability of extracellular RNAs (exRNAs) in biofluids has led to an emerging recognition of their promise as 'liquid biopsies' for diseases.
Kirsty M Danielson +4 more
doaj +1 more source
Cell, 2020 The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 ...
Yunlong Cao +33 more
semanticscholar +1 more source
High‐Throughput Multiplex Sequencing of miRNA [PDF]
Current Protocols in Human Genetics, 2012 AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a ...
Francois, Vigneault +7 more
openaire +2 more sources
HTSeq—a Python framework to work with high-throughput sequencing data
bioRxiv, 2014 Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard work flows, custom scripts are needed. Results: We present HTSeq, a Python library
S. Anders, P. Pyl, W. Huber
semanticscholar +1 more source
Molecular & Cellular Proteomics, 2021 Understanding the dynamics of the human proteome is crucial for developing biomarkers to be used as measurable indicators for disease severity and progression, patient stratification, and drug development.
Lotta Wik +11 more
semanticscholar +1 more source
Endogenous RNAs Modulate MicroRNA Sorting to Exosomes and Transfer to Acceptor Cells
Cell Reports, 2014 MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of
Mario Leonardo Squadrito +7 more
doaj +1 more source
Phage ImmunoPrecipitation Sequencing (PhIP-Seq): The Promise of High Throughput Serology
Pathogens, 2022 Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a high throughput serological technology that is revolutionizing the manner in which we track antibody profiles. In this review, we mainly focus on its application to viral infectious diseases.
Charles Kevin Tiu +3 more
doaj +1 more source
ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data
Nucleic Acids Research, 2010 High-throughput sequencing platforms are generating massive amounts of genetic variation data for diverse genomes, but it remains a challenge to pinpoint a small subset of functionally important variants.
Kai Wang, Mingyao Li, H. Hakonarson
semanticscholar +1 more source