Results 11 to 20 of about 15,584,188 (261)

High-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis

open access: yesDrug Design, Development and Therapy, 2015
Aicui Liu,1,2,* Chao Wang,1,2,* Zhijuan Liang,3 Zhi-Wei Zhou,4 Lin Wang,1,2 Qiaoli Ma,1,2 Guowei Wang,1,2 Shu-Feng Zhou,4 Zhenhai Wang1,2 1Neurology Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia; 2Key Laboratory of Brain ...
Liu A   +8 more
doaj   +1 more source

Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples. [PDF]

open access: yes, 2012
peer-reviewedBackground: The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an ...
Magee, David A   +52 more
core   +1 more source

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]

open access: yes, 2014
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A   +83 more
core   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

open access: yes, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J.   +27 more
core   +2 more sources

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

open access: yes, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg   +27 more
core   +1 more source

High throughput error corrected Nanopore single cell transcriptome sequencing

open access: yesNature Communications, 2020
Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full
Kevin Lebrigand   +3 more
doaj   +1 more source

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

open access: yes, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD   +21 more
core   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

open access: yesAnimals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul   +6 more
doaj   +1 more source

PIMS sequencing extension:a laboratory information management system for DNA sequencing facilities [PDF]

open access: yes, 2011
Background: Facilities that provide a service for DNA sequencing typically support large numbers of users and experiment types. The cost of services is often reduced by the use of liquid handling robots but the efficiency of such facilities is hampered ...
McPherson, MJ   +18 more
core   +1 more source

High-Throughput Microdissection for Next-Generation Sequencing. [PDF]

open access: yesPLoS ONE, 2016
Precision medicine promises to enhance patient treatment through the use of emerging molecular technologies, including genomics, transcriptomics, and proteomics.
Avi Z Rosenberg   +17 more
doaj   +1 more source

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