Results 61 to 70 of about 18,266,081 (302)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Analytic Correlation Filtration: A New Tool to Reduce Analytical Complexity of Metabolomic Datasets

Metabolites, 2019
Metabolomics generates massive and complex data. Redundant different analytical species and the high degree of correlation in datasets is a constraint for the use of data mining/statistical methods and interpretation.
Stephanie Monnerie, Melanie Petera, Bernard Lyan, Pierrette Gaudreau, Blandine Comte, Estelle Pujos-Guillot   +5 more
doaj   +1 more source

Non-targeted screening of emerging contaminants in drinking water based on high-resolution mass spectrometry

Shanghai yufang yixue, 2023
ObjectiveTo establish a non-targeted screening method for emerging contaminants in drinking water based on high-resolution mass spectrometry and apply it to actual water samples.MethodsA total of 9 drinking water samples collected from 3 reservoirs in ...
WU Linying, FENG Chao, WANG Guoquan, XUAN Dongliang, JIN Yu’e, PENG Qian, LU Dasheng   +6 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Determination of Synthetic Cathinones in Urine and Oral Fluid by Liquid Chromatography High-Resolution Mass Spectrometry and Low-Resolution Mass Spectrometry: A Method Comparison

Separations, 2020
Synthetic cathinones have become very popular recreational drugs. Therefore, determining them in biological samples is now a matter of concern. In recent years, different methods that have been developed can determine these drugs at low-concentration ...
Sergi Pascual-Caro, Francesc Borrull, Carme Aguilar, Marta Calull   +3 more
doaj   +1 more source

High-resolution mass spectrometry for detecting Acetylcholine in Arabidopsis [PDF]

Plant Signaling & Behavior, 2015
Acetylcholine (ACh) was first identified a century ago, and has long been known as a neurotransmitter in animals. However, it has been shown recently that the occurrence of ACh is widespread among various non-animal species including higher plants. Although previous reports suggest that various plant species are capable of responding to exogenously ...
Jun, Murata, Takehiro, Watanabe, Kohtaro, Sugahara, Tohru, Yamagaki, Toshio, Takahashi   +4 more
openaire   +2 more sources

Innovative solutions for enhanced illicit drugs profiling using comprehensive two-dimensional gas chromatography and mass spectrometry technologies [PDF]

, 2014
This project aimed to determine whether comprehensive two-dimensional gas chromatography is suitable for routine use in a forensic laboratory for profiling illicit substances.
Philip Marriot, Blagoj Mitrevski
core  

A critical evaluation of the current state-of-the-art in quantitative imaging mass spectrometry. [PDF]

, 2014
Mass spectrometry imaging (MSI) has evolved into a valuable tool across many fields of chemistry, biology, and medicine. However, arguably its greatest disadvantage is the difficulty in acquiring quantitative data regarding the surface concentration of ...
Ellis, Shane R., Heeren, Ron M. A., Heeren, R.M.A., Bruinen, A.L., Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics, Ellis, S.R., Bruinen, Anne L.   +7 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source