Results 81 to 90 of about 280,529 (308)

Analysis of Hydrogen Isotope Abundance by High Resolution Isotope Ratio Mass Spectrometry

Zhipu Xuebao
High resolution isotope ratio mass spectrometry (HR-IRMS) is often used to measure the abundance of deuterium to verify the efficiency of isotope isolation of a cryogenic distillation column system.
Chao WU, Shi-lin HU, Ying REN, Yan LIU, Bin-yong ZHANG, Yu-fei WANG   +5 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC–MS and LC-HRMS for Metabolic Flux Analysis

Data in Brief, 2017
The data presented in this article are related to the research article entitled “13C labeling analysis of sugars by high resolution-mass spectrometry for Metabolic Flux Analysis” (Acket et al., 2017) [1].
Sébastien Acket, Anthony Degournay, Franck Merlier, Brigitte Thomasset   +3 more
doaj   +1 more source

Probing the Composition, Assembly and Activity of Protein Molecular Machines using Native Mass Spectrometry

, 2017
Native mass spectrometry and mass spectrometry in general, are powerful analytical tools for studying proteins and protein complexes. Native mass spectrometry may provide accurate mass measurements of large macromolecular assemblies enabling the ...
van de Waterbeemd, M.J., Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics   +2 more
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Identification of Trenbolone Metabolites Using Hydrogen Isotope Ratio Mass Spectrometry and Liquid Chromatography/High Accuracy/High Resolution Mass Spectrometry for Doping Control Analysis

Frontiers in Chemistry, 2020
Trenbolone is a synthetic anabolic-androgenic steroid, which has been misused for performance enhancement in sports. The detection of trenbolone doping in routine sports drug testing programs is complex as methods utilizing gas chromatography/mass ...
Marlen Putz, Thomas Piper, Mario Thevis
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

The Mass Resolution of Ca, K Isotopes and CO, N2 and C2H4 Isobars in Isotopes Separator On-Line trap Mass spectrometry

Journal of Nigerian Society of Physical Sciences, 2020
In Isotopes separator on-line trap, ions are trapped, cooled, accumulated, bunched and isotopes or isobars are separated, cyclotron frequencies are determined, which are followed by time of flight mass resolution.
Dyavappa B M
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Integrative Mass Spectrometry Approaches to Monitor Protein Structures, Modifications, and Interactions

, 2017
This thesis illustrates the current standing of mass spectrometry (MS) in molecular and structural biology. The primary aim of the herein described research is to facilitate protein characterization by combining mass spectrometric methods among each ...
Lössl, P., Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics   +2 more
core