Results 91 to 100 of about 530,809 (300)

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Shotgun lipidomic analysis of chemically sulfated sterols compromises analytical sensitivity:Recommendation for large-scale global lipidome analysis [PDF]

, 2014
Shotgun lipidomics affords comprehensive and quantitative analysis of lipid species in cells and tissues at high-throughput [1 5]. The methodology is based on direct infusion of lipid extracts by electrospray ionization (ESI) combined with tandem mass ...
Casanovas, Albert, Ejsing, Christer S, Hannibal-Bach, Hans Kristian, Jensen, Ole Nørregaard   +3 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Identification of Trenbolone Metabolites Using Hydrogen Isotope Ratio Mass Spectrometry and Liquid Chromatography/High Accuracy/High Resolution Mass Spectrometry for Doping Control Analysis

Frontiers in Chemistry, 2020
Trenbolone is a synthetic anabolic-androgenic steroid, which has been misused for performance enhancement in sports. The detection of trenbolone doping in routine sports drug testing programs is complex as methods utilizing gas chromatography/mass ...
Marlen Putz, Thomas Piper, Mario Thevis
doaj   +1 more source

Desorption electrospray ionization-high resolution mass spectrometry for the screening of veterinary drugs in cross contaminated feddstuffs [PDF]

, 2016
In this study, a desorption electrospray ionization-high resolution mass spectrometry (DESI-HRMS) screening method was developed for fast identification of veterinary drugs in cross-contaminated feedstuffs. The reliable detection was performed working at
Bosch i Genover, Jaume, Galcerán Huguet, M. Teresa, Grases, Josep M., Moyano Morcillo, Encarnación, Núñez Burcio, Oscar, Rodríguez, Pilar, Seró Llor, Raquel   +6 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The Mass Resolution of Ca, K Isotopes and CO, N2 and C2H4 Isobars in Isotopes Separator On-Line trap Mass spectrometry

Journal of Nigerian Society of Physical Sciences, 2020
In Isotopes separator on-line trap, ions are trapped, cooled, accumulated, bunched and isotopes or isobars are separated, cyclotron frequencies are determined, which are followed by time of flight mass resolution.
Dyavappa B M
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Ultra High-Mass Resolution Paper Spray by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

International Journal of Analytical Chemistry, 2012
Paper Spray Ionization is an atmospheric pressure ionization technique that utilizes an offline electro-osmotic flow to generate ions off a paper medium.
Kevin D. Quinn, Charmion I. Cruickshank, Troy D. Wood   +2 more
doaj   +1 more source

From Single Constituents to Metabolomics in Food Quality Analysis [PDF]

, 2013
Until now, a wide range of laboratory approaches has been employed for control of organic food quality and authenticity. In addition to widely used light isotope measurement based methods, various spectroscopic and/or chromatographic have been ...
Hajslova, Jana, Schulzova, Vera
core