Results 41 to 50 of about 164 (159)

PSMD14 Stabilizes SLC7A11 to Ameliorate Glucocorticoid‐Induced Osteoporosis by Suppressing Osteocyte Ferroptosis

Advanced Science, EarlyView.
Glucocorticoid‐induced osteoporosis (GIOP) triggers osteocyte ferroptosis via SLC7A11 degradation. PSMD14 stabilizes SLC7A11 by counteracting glucocorticoid‐driven ubiquitination, preserving cystine uptake and glutathione synthesis. AAV‐mediated PSMD14 delivery or its agonist Pantethine rescues osteocyte survival and bone loss in GIOP mice.
Yifeng Shi, Qian Tang, Sunren Sheng, Hongyi Jiang, Chen Jin, Chencheng Zhou, Chenglong Xie, Lin Zheng, Di Zhang, Hui Xu, Cong Xu, Haiwei Ma, Guangheng Xiang, Wenfei Ni, Xiaoyun Pan, Lei Yang, Huazi Xu, Yu Qian, Aimin Wu, Xiangyang Wang, Gang Zheng   +20 more
wiley   +1 more source

Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility

Advanced Science, EarlyView.
The molecular recognition of the sugar moiety of GD3 and Gb3 (globotriaosylceramide) derivatives by Siglec‐7 has been thoroughly studied offering insights for cancer diagnostics and therapies. A combination of structural biology techniques, nuclear magnetic resonance (NMR) spectroscopy, physicochemical analysis, and in silico methods suggested that ...
Cristina Di Carluccio, Luis Padilla‐Cortés, Marta Tiemblo‐Martìn, Giulia Roxana Gheorghita, Rosario Oliva, Linda Cerofolini, Alessandro Antonio Masi, Celeste Abreu, Hsin‐Kai Tseng, Antonio Molinaro, Pompea Del Vecchio, Ondřej Vaněk, Chun‐Cheng Lin, Roberta Marchetti, Marco Fragai, Alba Silipo   +15 more
wiley   +1 more source

Structural and Biochemical Characterization of a Widespread Enterobacterial Peroxidase Encapsulin

Advanced Science, EarlyView.
Encapsulins are self‐assembling bacterial protein compartments loaded with cargo enzymes. The most abundant encapsulin cargo class are Dye‐decolorizing Peroxidases (DyPs). In this study, we structurally and biochemically characterize a DyP encapsulin found in many enterobacteria.
Natalia C. Ubilla‐Rodriguez, Michael P. Andreas, Tobias W. Giessen   +2 more
wiley   +1 more source

Asprosin‐FABP5 Interaction Modulates Mitochondrial Fatty Acid Oxidation through PPARα Contributing to MASLD Development

Advanced Science, EarlyView.
Aided by FABP5, abnormally elevated asprosin in hepatocytes enters the nucleus, targets and inhibits PPARα binding to the CPT1A promoter, thereby suppressing FAO. Circulating asprosin exacerbates insulin resistance, collectively driving MASLD progression.
Yuan‐Yuan Yu, Min Feng, Yi Chen, Hong‐Lin Jia, Qi Zhang, Ming Tong, Yan‐Xi Li, Yu Zhao, Xin‐Xin Liu, Shi‐Feng Cao, Zheng‐kai Wang, Hou‐wei Li, Xue Liu, Yan Zhang   +13 more
wiley   +1 more source

Cardiomyocyte‐Enriched USP20 Ameliorates Pathological Cardiac Hypertrophy by Targeting STAT3 Deubiquitination

Advanced Science, EarlyView.
Cardiomyocyte‐enriched USP20 regulates the K63‐linked ubiquitination of STAT3, and the cardiomyocyte‐specific USP20‐STAT3‐CARM1 axis exerts a protective role in cardiac hypertrophy. Targeting USP20 through cardiac‐specific gene therapy presents a promising strategy for the treatment of cardiac hypertrophy.
Lingfeng Zhong, Shanshan Dai, Fan Yu, Guo‐Ping Shi, Qinyan Gong, Yucong Zhang, Jingsi Duan, Zhengyin Lou, Zhixuan Tang, Fuzhe Gong, Derong Chen, Liya Hou, Xinyang Hu, Jinghai Chen, Jian'an Wang, Deling Yin   +15 more
wiley   +1 more source

Rapid and Noninvasive Early Detection of Lung Cancer by Integration of Machine Learning and Salivary Metabolic Fingerprints Using MS LOC Platform: A Large‐Scale Multicenter Study

Advanced Science, EarlyView.
Salivary metabolic signatures associated with lung cancer are systematically characterized through a self‐developed high‐throughput mass spectrometry Lab‐on‐a‐Chip platform. Utilizing these metabolic fingerprints, SalivaMLD, an integrated machine‐learning‐driven classification model is developed and demonstrates superior performance in detecting lung ...
Shuang Lin, Runlan Yan, Junqi Zhu, Bei Li, Yinyan Zhong, Shuang Han, Huiting Wang, Jianmin Wu, Zhao Chen, Yuyue Jiang, Aiwu Pan, Xuqing Huang, Xiaoming Chen, Pingya Zhu, Sheng Cao, Wenhua Liang, Peng Ye, Yue Gao   +17 more
wiley   +1 more source

Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes

Advanced Science, EarlyView.
Ethylene‐activated MdEAEL1 mediates the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex, upregulating the histone acetylation levels in the promoter regions of cell wall degradation‐related genes, consequently promoting softening during storage.
Tong Li, Li Liu, Guangxin Yang, Yingcong Cai, Yingda Wang, Bowen Sun, Le Sun, Weiting Liu, Aide Wang   +8 more
wiley   +1 more source

Enhancing the Protein Stability of an Anticancer VHH‐Fc Heavy Chain Antibody through Computational Modeling and Variant Design

Advanced Science, EarlyView.
In this study, a computational pipeline is developed to systematically evaluate the conformational stability, disulfide bond reduction state, and aggregation and degradation tendencies of an anticancer VHH‐Fc fusion antibody. Based on the mechanistic insights, antibody variants with enhanced stability and increased yield are successfully designed. This
Yuan Fang, Menghua Song, Tianning Pu, Xiaoqing Song, Kailu Xu, Pengcheng Shen, Ting Cao, Yiman Zhao, Simon Hsu, Dongmei Han, Qiang Huang   +10 more
wiley   +1 more source

Explainable Deep Multilevel Attention Learning for Predicting Protein Carbonylation Sites

Advanced Science, EarlyView.
Selective carbonylation sites (SCANS) are conceptualized, designed, evaluated, and released. SCANS captures segment‐level, protein‐level, and residue embeddings features. It utilizes elaborate loss function to penalize cross‐predictions at the residue level.
Jian Zhang, Jingjing Qian, Pei Wang, Xuan Liu, Fuhao Zhang, Haiting Chai, Quan Zou   +6 more
wiley   +1 more source

The MdHB7L–MdICE1L–MdHOS1 Module Fine‐Tunes Apple Cold Response via CBF‐Dependent and CBF‐Independent Pathways

Advanced Science, EarlyView.
In early cold response, MdICE1L utilizes MdHB7L as a cofactor to facilitate the transcriptional activation of MdCBFs, thereby activating cold signaling rapidly and strongly. Subsequently, MdICE1L is degraded by MdHOS1. Meanwhile, MdHB7L is released and accumulates.
Jie Yang, Na Li, Ming Li, Ran Yi, Lina Qiu, Kangning Wang, Shuang Zhao, Fengwang Ma, Ke Mao   +8 more
wiley   +1 more source