Results 41 to 50 of about 186,634 (241)

Advances in biological functions and mechanisms of histone variants in plants

Frontiers in Genetics, 2023
Nucleosome is the basic subunit of chromatin, consisting of approximately 147bp DNA wrapped around a histone octamer, containing two copies of H2A, H2B, H3 and H4.
Xi Wu, Xu Zhang, Borong Huang, Junyou Han, Huihui Fang   +4 more
doaj   +1 more source

Polycistronic coexpression and nondenaturing purification of histone octamers [PDF]

Analytical Biochemistry, 2012
Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions.
Yoonjung, Shim, Ming-Rui, Duan, Xuejing, Chen, Michael J, Smerdon, Jung-Hyun, Min   +4 more
openaire   +2 more sources

N-Terminal Tails of Histones H2A and H2B Differentially Affect Transcription by RNA Polymerase II In Vitro

Cells, 2022
Histone N-terminal tails and their post-translational modifications affect various biological processes, often in a context-specific manner; the underlying mechanisms are poorly studied.
Han-Wen Chang, Alexey V. Feofanov, Alexander V. Lyubitelev, Grigory A. Armeev, Elena Y. Kotova, Fu-Kai Hsieh, Mikhail P. Kirpichnikov, Alexey K. Shaytan, Vasily M. Studitsky   +8 more
doaj   +1 more source

Probing the (H3-H4)(2) histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling [PDF]

, 2009
The (H3-H4)2 histone tetramer forms the central core of nucleosomes and, as such, plays a prominent role in assembly, disassembly and positioning of nucleosomes.
Bowman, A., El-Mkami, Hassane, Norman, David G., Owen-Hughes, Tom, Ward, Richard   +4 more
core   +5 more sources

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers [PDF]

, 2010
Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligomeric
A. Sawasdichai, A. Shukla, A. Soufi, Bess, Butcher, C. Smith, Cai, Cairns, Crompton, D'Elia, Dafforn, de Los Rios, Desjobert, Galande, Gaston, Gaston, Gaston, Goldman, Jennings, K. Gaston, Katsani, Larquet, Lennard, Luger, Luijsterburg, Martino, Mohd-Sarip, P. Noy, P.-S. Jayaraman, Puppin, Sekiya, Song, Soufi, Soufi, Soufi, T. Dafforn, Tanny, Thaw, Topisirovic, Vernon, Williams, Yasui   +41 more
core   +6 more sources

Neurogenesis and the Epigenetic Landscape: Role of Histone Modifications and Chromatin Remodeling. [PDF]

Brain Behav
Histone methylation and acetylation modulate gene expression by inducing chromatin condensation or relaxation, which in turn regulates transcriptional activity and impacts neurogenesis. ABSTRACT Aims The purpose of this review is to examine how epigenetic regulation particularly chromatin modification and histone methylation controls gene expression ...
Mengistu DY, Eskeziyaw BM.
europepmc   +2 more sources

TRF1 and TRF2 binding to telomeres is modulated by nucleosomal organization [PDF]

, 2015
The ends of eukaryotic chromosomes need to be protected from the activation of a DNA damage response that leads the cell to replicative senescence or apoptosis.
Alicata, Claudia, Cacchione, Stefano, Cicconi, Alessandro, Galati, Alessandra, Gilson, Eric, Giraud Panis, Marie Josèphe, Ingegnere, Tiziano, Micheli, Emanuela, Pusch, Miriam Caroline   +8 more
core   +3 more sources

Simplified Method for Rapid Purification of Soluble Histones

Croatica Chemica Acta, 2016
Functional and structural studies of histone-chaperone complexes, nucleosome modifications, their interactions with remodelers and regulatory proteins rely on obtaining recombinant histones from bacteria.
Nives Ivić, Bernhard Groschup, Silvija Bilokapić, Mario Halić   +3 more
doaj   +1 more source

Characteristic H3 N-tail dynamics in the nucleosome core particle, nucleosome, and chromatosome

iScience, 2022
Summary: The nucleosome core particle (NCP) comprises a histone octamer, wrapped around by ∼146-bp DNA, while the nucleosome additionally contains linker DNA.
Ayako Furukawa, Masatoshi Wakamori, Yasuhiro Arimura, Hideaki Ohtomo, Yasuo Tsunaka, Hitoshi Kurumizaka, Takashi Umehara, Yoshifumi Nishimura   +7 more
doaj   +1 more source

Folding of single‐stranded DNA on the histone octamer [PDF]

FEBS Letters, 1985
A complex between the single‐stranded DNA of the bacteriophage M13 and the histone octamer was analyzed by electron microscopy, low‐angle X‐ray diffraction and nuclease analysis. The morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome.
Caffarelli E, Leoni L, Savino M
openaire   +3 more sources