Results 41 to 49 of about 50 (49)
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Attraction, phasing and neighbour effects of histone octamers on curved DNA
Journal of Molecular Biology, 1990Nucleosome core particles were reconstituted on various DNA fragments containing a Crithidia fasciculata kinetoplast curved tract. The results show that, on curved DNA, nucleosome core particles form six- to sevenfold preferentially, relative to bulk sequences.
G. COSTANZO+3 more
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The oxidised histone octamer does not form a H3 disulphide bond
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2006A H3 dimer band is produced when purified native histone octamers are run on an SDS-PAGE gel in a beta-mercaptoethanol-free environment. To investigate this, native histone octamer crystals, derived from chicken erythrocytes, and of structure (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A'), were grown in 2 M KCl, 1.35 M potassium phosphates and 250-350 microM ...
Colin D. Reynolds+5 more
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Biochemistry, 2005
Using a novel competition assay to determine the relative strength of different histone octamer-binding sites, we have compared three natural and two synthetic sites. We show that the relative affinities of these sites for the histone octamer depend upon both the temperature and octamer concentration.
Chenyi Wu, Andrew Travers
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Using a novel competition assay to determine the relative strength of different histone octamer-binding sites, we have compared three natural and two synthetic sites. We show that the relative affinities of these sites for the histone octamer depend upon both the temperature and octamer concentration.
Chenyi Wu, Andrew Travers
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Structural similarity between TAFs and the heterotetrameric core of the histone octamer
Nature, 1996A complex of two TFIID TATA box-binding protein-associated factors (TA FIIs) is described at 2.0A resolution. The amino-terminal portions of dTAFII42 and dTAFII62 from Drosophila adopt the canonical histone fold, consisting of two short alpha-helices flanking a long central alpha-helix.
Tetsuro Kokubo+12 more
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Effects of DNA topology in the interaction with histone octamers and DNA topoisomerase I
Biophysical Chemistry, 1994Several simple proteins and complex protein systems exist which do not recognize a defined sequence but--rather--a specific DNA conformation. We describe experiments and principles for two of these systems: nucleosomes and eukaryotic DNA topoisomerase I.
NEGRI, RODOLFO+4 more
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Advanced Glycation of Rat Liver Histone Octamers: An in Vitro Study
Biochemical and Biophysical Research Communications, 1994The question whether histones could accumulate AGE products and whether this reaction could take place even on the native histone octamer, the basic nucleosome unit, was addressed in this study. In vitro AGE formation on rat liver native histones octamers by incubation with different sugars was assessed.
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[Structure of histone octamers in reconstituted polynucleosomes].
Molekuliarnaia biologiia, 1986The salt-dependent structural changes of the histone octamer in complex with high-molecular-weight DNA have been studied by fluorescent spectroscopy. Changes in both the spectra maximum position and anisotropy of the histone tyrosine fluorescence reveal structural transitions in nucleosome within the ranges of 0.5-3 mM and 20-30 mM NaCl.
S N, Khrapunov+3 more
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