Results 101 to 110 of about 308,174 (298)

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Antiretroviral Drug Resistance Testing in Adult HIV-1 Infection: 2008 Recommendations of an International AIDS Society-USA Panel [PDF]

, 2017
Resistance to antiretroviral drugs remains an important limitation to successful human immunodeficiency virus type 1 (HIV-1) therapy. Resistance testing can improve treatment outcomes for infected individuals.
Clotet, Bonaventura, Günthard, Huldrych F., Hammer, Scott M., Hirsch, Martin S., Jacobsen, Donna M., Johnson, Victoria A., Kuritzkes, Daniel R., Mellors, John W., Pillay, Deenan, Richman, Douglas D., Schapiro, Jonathan M., Vézinet, Françoise Brun, Yeni, Patrick G.   +12 more
core  

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, EarlyView.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source

High prevalence of HIV-1 transmitted drug resistance among therapy-naïve Burmese entering travelers at Dehong ports in Yunnan, China

BMC Infectious Diseases, 2018
Background The overall success of Human immunodeficiency virus type 1 (HIV-1) antiretroviral therapy (ART) was heavily challenged upon the occurrence of drug resistance.
Qicai Xuan, Shuwen Liang, Weihong Qin, Shuting Yang, A-mei Zhang, Ting Zhao, Hui Su, Zhiqing Xia, Binghui Wang, Xueshan Xia   +9 more
doaj   +1 more source

Prevalence of human immunodeficiency virus-1 drug-resistant mutations among adults on first- and second-line antiretroviral therapy in a resource-limited health facility in Busia County, Kenya

The Pan African Medical Journal, 2020
INTRODUCTION: In Kenya, about 1.5 million people are living with the Human Immunodeficiency Virus (HIV). Antiretroviral therapy aids in viral suppression. However, drug-resistance threaten the gains of the HIV infection control program.
Olipher Makwaga, Maureen Adhiambo, David Hughes Mulama, John Muoma, Ferdinard Adungo, Humphrey Wanjiku, Asiko Ongaya, Geoffrey Mutisya Maitha, Matilu Mwau   +8 more
doaj   +1 more source

HIV drug resistance in HIV-infected children

Jornal de Pediatria, 2009
Almeida et al. report, in this issue of the journal, a retrospective studyon theprevalence and patterns of antiretroviral drug resistance in 24 treatment-naive and 23 treatment-experienced children failing therapy between 2000 and 2004 attending a clinic in Sao Paulo.
openaire   +2 more sources

Rational Design of Broad‐Spectrum Anti‐Enteroviral Molecular Glues Targeting Enteroviral RNAi Suppressors

Advanced Science, EarlyView.
By leveraging this homodimerization mechanism, molecular glues were rationally designed to induce dysfunctional 3A dimerization, thereby restoring antiviral RNAi. The optimal molecular glue, VTP‐32, demonstrated potent and pan‐enterovirus (groups A, B, D) antiviral effects.
Yuan Fang, Xiong Xie, Huidi Fan, Botao Wu, An Wang, Wenhao Dai, Zezhong Liu, Jian Li, Huoyan Tong, Jianan Li, Yujie Ren, Jinlin Wang, Xi Zhou, Hong Liu   +13 more
wiley   +1 more source

Development of a Human 3D Immune‐Competent Neurovascular Model Enabling Time‐Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions

Advanced Science, EarlyView.
Neuroinflammation alters blood–brain barrier integrity and contributes to neurological disorders, yet existing models lack human immune complexity. This study presents a 3D, immune‐responsive platform that reconstructs key neurovascular components and enables real‐time monitoring.
Pavlo Gordiichuk, Jing Bai, Olurotimi A. Bolonduro, Andrew M. Silverman, Robert Madison Green, Jason H. Lasser, Paul W. Fleming, Lishomwa C. Ndhlovu, James F. Saunders, Dmitry Shvartsman   +9 more
wiley   +1 more source

TYPICAL FEATURES OF PRIMARY DRUG RESISTANCE AND VIABILITY OF MYCOBACTERIA IN TUBERCULOSIS PATIENTS WITH CONCURRENT HIV INFECTION

Туберкулез и болезни лёгких, 2017
Goal: to study the structure, profile of primary drug resistance and viability of mycobacteria in tuberculosis patients with concurrent HIV infection.Materials and methods.
N. M. Koretskaya, I. A. Bolshakova
doaj   +1 more source