Results 201 to 210 of about 4,391,067 (296)

Oligonucleotide Ligation Assay (OLA)-Simple: Field Implementation, Usability, and Performance of a near Point-of-Care HIV Drug Resistance Assay in Kenya. [PDF]

Laboratories
Owiti PO, Chohan BH, Beck IA, Panpradist N, Maheria P, Thomas KK, Giang JH, Kingwara L, Onwonga VM, Madada RS, Akasa S, Akinyi G, Opollo V, Kiiru J, Bowen N, Samadpour M, Basha GW, Lutz BR, Frenkel LM, Oyaro P, Abuogi LL, Patel RC.   +21 more
europepmc   +1 more source

Stoichiometric Binding of Cyclophilin‐A to The HIV‐1 Capsid Modulates Its Mechanoelastic Properties

Small, EarlyView.
The HIV‐1 capsid is a protein nanocapsule that must balance rigidity and flexibility to enter the cell nucleus. Atomic force microscopy simulations reveal that binding of the host protein Cyclophilin A tunes this mechanical response: partial coverage preserves deformability, while excessive binding induces brittleness and failure.
Juan S. Rey, Alexander J. Bryer, Juan R. Perilla   +2 more
wiley   +1 more source

Rapid and Efficient Affinity Isolation of Engineered Extracellular Vesicles Encapsulating Functional Proteins via Internal Twin‐Strep Tetraspanin Tagging

Small, EarlyView.
Structure‐guided internal twin‐Strep tagging of the tetraspanins CD63 or CD9 enables rapid and efficient antibody‐free affinity isolation of engineered extracellular vesicles (EVs) using Strep technology while allowing luminal protein loading. The approach removes over 96% of contaminants, separates tagged EVs from coexisting viral particles, and ...
Minh‐Tu Pham, Jona Benjamin Krohn, Janis Meyer, Christian Ritter, Martin Schneider, Dominic Helm, Dario Frey, Kleopatra Rapti, Uta Haselmann‐Weiss, Elif Toprak, Viet Loan Dao Thi, Florian Leuschner, Dirk Grimm, Karl Rohr, Ralf Bartenschlager   +14 more
wiley   +1 more source

An audit of the HIV drug resistance testing program in Malawi. [PDF]

Malawi Med J
Kanise H, Nyirenda K, Bisani P, Matola BW, Makwaya A, Simon K, Cox C, Hosseinipour MC, Kanyama C, Worku A, Phiri S, Hoffman RM, Nyirenda R, Heller T, van Oosterhout JJ.   +14 more
europepmc   +1 more source

Artificial intelligence empowers targeted protein degradation: Core technological innovations, multi‐scenario applications, and translational prospects

Smart Molecules, EarlyView.
AI is transforming TPD by improving the design, prediction, and optimization of degraders such as PROTACs, molecular glues, and LYTACs. This review summarizes key AI‐driven advances, highlights applications across drug discovery stages, and discusses remaining challenges and future directions for accelerating the development of therapies against ...
Shuanglin Qin, Rui Peng, Guangshuai Zhang, Si Yan, Jin Xiao, Zishu Liu, Ziyu Tan, Qingqing Xie, Shangjie Li, Wei Luo, Xiaohe Xiao   +10 more
wiley   +1 more source

Molecular glues: Recent advances in cereblon substrate identification and mechanistic insights

Smart Molecules, EarlyView.
Molecular glues exert diverse core biological functions by inducing or stabilizing protein–protein interactions, extending far beyond the traditional scope of targeted protein degradation. Key breakthroughs include expanded cereblon substrate recognition, sustained G protein‐coupled receptor megacomplex signaling, and the first orthosteric molecular ...
Qingyun Guan, Xiaoyu Shi, Qian Yang, Xiangyi Jiang, Xinyong Liu, Peng Zhan   +5 more
wiley   +1 more source

National representative survey of pretreatment HIV drug resistance in Colombia: analysis of antiretroviral resistance-associated mutations using next-generation sequencing. [PDF]

J Antimicrob Chemother
Martínez-Buitrago E, Zapata-Marín AF, Rubiano LC, Sañudo LM, Oñate JM, Lenis W, García-Góez JF, Álvarez JE, García-Morales C, Matías-Florentino M, Ávila-Ríos S.   +10 more
europepmc   +1 more source

Microfluidic Molecular Diagnostic Chips: Functional Modules, Integration Strategies, and Emerging Applications

Small Methods, EarlyView.
This comprehensive review delivers an engineering‐centric analysis of microfluidic molecular diagnostic chips, breaking them down into foundational modules from fluid manipulation to bio‐sensing. Beyond chip design, it evaluates sample‐to‐answer integration strategies and commercial readiness levels.
Donghoon Koo, Egor Kokin, Sangyul Baik, Chanyong Park, Minji Kim, Jeongmin Kim, Ryungeun Song, Hosub Lim   +7 more
wiley   +1 more source

Prevalence, associated factors and geospatial patterns of HIV drug resistance in South Africa among the virally unsuppressed adults: updated results from the 2017 national HIV household survey. [PDF]

BMC Public Health
Seleka M, Ledibane N, Mokhele T, Zuma K, Moyo S, Jooste S, Musekiwa A.   +6 more
europepmc   +1 more source

“Who Is Watching me?”: Recovering the Grammar of Stigma in Interaction

Symbolic Interaction, EarlyView.
This article offers a praxeological respecification of stigma. I argue that the term “stigma” is a category term and glossing device, used to gather together and stand in for a diverse range of interactional experiences, including, but not limited to, degradation, discrediting, discrimination, marginalization, and being demeaned.
Phil Hutchinson
wiley   +1 more source