Results 201 to 210 of about 73,667 (245)

Colorimetric reverse transcriptase assay for HIV-1

Journal of Virological Methods, 1993
A colorimetric assay for reverse transcriptase (RT) of the human immunodeficiency virus type 1 (HIV-1) was developed using a double labelled (biotin and digoxigenin) deoxyuridine triphosphate mixture instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from oligodeoxythymidylic acid (oligo-dT) contained both ...
K, Suzuki, B P, Craddock, T, Kano, R T, Steigbigel   +3 more
openaire   +2 more sources

Chemiluminescent Enzyme-Linked Immunoassay for Reverse Transcriptase, Illustrated by Detection of HIV Reverse Transcriptase

Analytical Biochemistry, 1993
A chemiluminescent assay for reverse transcriptase (RT) of the human immunodeficiency virus 1 was developed using biotin-labeled oligodeoxythymidylic acid (biotin oligo-dT) and digoxigenin-deoxyuridine triphosphate instead of tritiated thymidine triphosphate. After the RT reaction, the newly polymerized strand from biotin oligo-dT contained digoxigenin
K, Suzuki, B P, Craddock, T, Kano, R T, Steigbigel   +3 more
openaire   +2 more sources

HIV-1 Reverse Transcriptase Structures [PDF]

, 2013
© 2013 Elsevier Inc. All rights reserved. The reverse transcriptase (RT) of human immunodeficiency virus (HIV) is the multifunctional enzyme responsible for the conversion of the single-stranded viral RNA genome into double-stranded DNA (dsDNA) that is ...
K. Das, E. Arnold, S.H. Hughes
openaire   +2 more sources

Novel HIV-1 reverse transcriptase inhibitors

Virus Research, 2008
HIV-1 reverse transcriptase (RT) was the first viral enzyme to be targeted by anti-HIV drugs. Despite 20 years of experience with RT inhibitors, new ways to inhibit this target and address viral resistance continue to emerge. In both licensed RT inhibitor classes, nucleosides (NRTIs) and non-nucleosides (NNRTIs), compounds with better resistance ...
openaire   +2 more sources

HIV and reverse transcriptase inhibition by tannins

Bioorganic & Medicinal Chemistry Letters, 1992
Abstract Further evaluation of tannins as anti-HIV agents indicates that these compounds inhibited HIV replication only slightly in the absence of toxicity (therapeutic index ≤ 5). In addition, no correlation was found between inhibition of reverse transcriptase and of HIV in cell culture.
Robert E. Kilkuskie, Yoshiki Kashiwada, Gen-ichiro Nonaka, Itsuo Nishioka, Anne J. Bodner, Yung-Chi Cheng, Kuo-Hsiung Lee   +6 more
openaire   +1 more source

HIV resistance to reverse transcriptase inhibitors

Biochemical Pharmacology, 1994
openaire   +2 more sources

Inhibitors of HIV reverse transcriptase

1994
WILLIAMS THERESA M, CICCARONE TERRENCE M, SAARI WALFRED S, WAI JOHN S, GREENLEE WILLIAM J, BALANI SURESH K, GOLDMAN MARK E, HOFFMAN JACOB M JR, LUMMA WILLIAM C JR, HUFF JOEL R, ROONEY CLARENCE S, SANDERSON PHILIP E, THEOHARIDES ANTHONY D DI   +12 more
openaire   +5 more sources

Specific HIV-1 Reverse Transcriptase Inhibitors

Journal of Enzyme Inhibition, 1992
Z, Debyser, R, Pauwels, K, Andries, E, De Clercq   +3 more
openaire   +2 more sources

Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA

EMBO Journal, 2001
Jianping Ding, Stefan G Sarafianos, Kalyan Das   +2 more
exaly  

The structure of HIV-1 reverse transcriptase complexed with an RNA pseudoknot inhibitor

EMBO Journal, 1998
Thomas Steitz, Tobias Restle, Joachim Jaeger   +2 more
exaly