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Sequencing-Based Typing of HLA
2007Human leukocyte antigen (HLA) typing is largely performed by use of PCR-based techniques in patients that require stem cell transplantation. In this chapter, HLA typing by sequencing-based typing techniques is described.
Berthold, Hoppe, Abdulgabar, Salama
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HLA-DR TYPING BY RADIOIMMUNOASSAY
Transplantation, 1980A radioimmunoassay procedure is described by which peripheral blood lymphocytes can be typed for HLA-DR specificities. The major advantages of this method are the following: simple and reproducible procedure, no need for B lymphocyte separation, no need for optimal viability, and no need for preabsorption of antisera with platelets.
R, Tosi +6 more
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2012
Human leukocyte antigen-E (HLA-E) is a non-classical HLA class I gene that shows a limited degree of polymorphism compared to the classical HLA genes. The HLA-E molecule can bind peptides derived from the leader sequence of various HLA class I alleles and some viral homologues, including CMV.
Nina, Lauterbach +2 more
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Human leukocyte antigen-E (HLA-E) is a non-classical HLA class I gene that shows a limited degree of polymorphism compared to the classical HLA genes. The HLA-E molecule can bind peptides derived from the leader sequence of various HLA class I alleles and some viral homologues, including CMV.
Nina, Lauterbach +2 more
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Multiplex HLA-Typing by Pyrosequencing
2009Class I and II loci of the human leukocyte antigens (HLA) represent the most polymorphic region of the genome. Evolutionary pressure has resulted in a large number of allelic variants of these loci ensuring the high frequency of heterozygous genotypes observed in human populations.
Ying, Lu +4 more
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Nature Medicine, 2000
Karen Birmingham's interesting article on the South African AIDS Initiative (Nature Med. 5, 1220; 1999) states that "the predominant HLA types in South Africa are presently unknown." This ignores a succession of International Histocompatibility Workshops that have provided both HLA serological and molecular typing data for many sub-Saharan African ...
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Karen Birmingham's interesting article on the South African AIDS Initiative (Nature Med. 5, 1220; 1999) states that "the predominant HLA types in South Africa are presently unknown." This ignores a succession of International Histocompatibility Workshops that have provided both HLA serological and molecular typing data for many sub-Saharan African ...
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Clinical Applications of HLA Typing
Clinics in Laboratory Medicine, 1982HLA typing has proved to be a valuable and versatile clinical tool, permitting more successful renal transplantation, platelet transfusion, and paternity testing, and even serving as a diagnostic test for ankylosing spondylitis. HLA typing of a family can indicate which relatives are at highest risk for diseases such as hemochromatosis and juvenile ...
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HLA-A and HLA-B typing: classification of errors.
Acta Universitatis Palackianae Olomucensis Facultatis Medicae, 1995The authors followed the frequency of errors in serologic identification of HLA-A and HLA-B antigens. They analyzed 205 paternity cases evaluated independently by two judicial experts (a revision of expert opinions). The specification of revealed discrepancies is demonstrated in the study and possible causes of their origin are analyzed.
E, Lenhartová +3 more
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