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Reduced dimensionality 3D HNCAN for unambiguous HN, CA and N assignment in proteins
Journal of Magnetic Resonance, 2012We present here an improvisation of HNN (Panchal, Bhavesh et al., 2001) called RD 3D HNCAN for backbone (HN, CA and (15)N) assignment in both folded and unfolded proteins. This is a reduced dimensionality experiment which employs CA chemical shifts to improve dispersion. Distinct positive and negative peak patterns of various triplet segments along the
Rout, Manoj Kumar +3 more
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BEST-HNN and 2D-(HN)NH experiments for rapid backbone assignment in proteins
Journal of Magnetic Resonance, 2010HNN has proven to be an extremely valuable experiment for rapid and unambiguous backbone (H(N), (15)N) assignment in ((13)C, (15)N) labeled proteins. However, low sensitivity of the experiment is often a limiting factor, especially when the transverse relaxation times (T(2)) are short. We show here that BEST modification Schanda et al.
Dinesh, Kumar +2 more
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The Fusion Promotion Activity of the NDV HN Protein Does Not Correlate with Neuraminidase Activity
Virology, 1993Three activities, attachment, neuraminidase, and fusion promotion, have been associated with the hemagglutinin-neuraminidase (HN) protein encoded by paramyxoviruses such as Newcastle disease virus. The fusion promotion activity of the HN protein can be separated from its attachment activity by mutation (Sergel et al., 1993, Virology 193, 717-726).
T, Sergel, L W, McGinnes, T G, Morrison
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Virology, 1993
To explore the role of the transmembrane domain of the HN protein in the structure and function of the molecule, three conserved leucine residues in this domain which occur in a heptad-repeat motif were changed to alanine singly or in combination by site-specific mutagenesis. None of the mutant proteins were defective in translocation and intracellular
L, McGinnes, T, Sergel, T, Morrison
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To explore the role of the transmembrane domain of the HN protein in the structure and function of the molecule, three conserved leucine residues in this domain which occur in a heptad-repeat motif were changed to alanine singly or in combination by site-specific mutagenesis. None of the mutant proteins were defective in translocation and intracellular
L, McGinnes, T, Sergel, T, Morrison
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A Selective Intra-HN(CA)CO Experiment for the Backbone Assignment of Deuterated Proteins
Journal of Biomolecular NMR, 2004A modified version of the backbone TROSY based 3D HN(CA)CO experiment is presented that provides uniquely intraresidue correlation shift information, by efficiently suppressing the sequential connectivity data. The resulting reduction in the number of peaks decreases overlap and removes ambiguities in situations where in the conventional HN(CA)CO i and
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Virology, 1990
The nucleotide sequence of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus (PIV-2) was determined. The PIV-2 HN gene was 2112 nucleotides excluding poly(A) tail. There was a single large open reading frame in the mRNA which encoded a protein of 571 amino acids with a calculated molecular weight of 63,262.
M, Kawano +7 more
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The nucleotide sequence of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus (PIV-2) was determined. The PIV-2 HN gene was 2112 nucleotides excluding poly(A) tail. There was a single large open reading frame in the mRNA which encoded a protein of 571 amino acids with a calculated molecular weight of 63,262.
M, Kawano +7 more
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Acta Crystallographica Section D Biological Crystallography, 1999
Botulinum neurotoxin (BoNT) is one of the most potent toxins known. BoNT is also a food poison, which means that the toxin must survive the protease action and acidity of the gut. A group of neurotoxin-associated proteins which are only beginning to be identified and characterized are believed to be responsible for this protection.
A T, Hadfield +3 more
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Botulinum neurotoxin (BoNT) is one of the most potent toxins known. BoNT is also a food poison, which means that the toxin must survive the protease action and acidity of the gut. A group of neurotoxin-associated proteins which are only beginning to be identified and characterized are believed to be responsible for this protection.
A T, Hadfield +3 more
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Antagonistic involvement of FIS and H‐NS proteins in the transcriptional control of hns expression
Molecular Microbiology, 1996Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns. These sites extend from −282 to +25 with two sites, closely flanking the DNA bend located at −150 from the transcriptional startpoint, partly overlapping the H‐NS binding
M, Falconi +4 more
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Journal of Biomolecular NMR, 2008
Alternate implementations of the SOFAST-HMQC experiment are described. In these alternate SOFAST-HMQC experiments (ALSOFAST-HMQC) excitation of the magnetization of interest is achieved by non-selective rf pulses while preserving the equilibrium polarization of passive spins.
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Alternate implementations of the SOFAST-HMQC experiment are described. In these alternate SOFAST-HMQC experiments (ALSOFAST-HMQC) excitation of the magnetization of interest is achieved by non-selective rf pulses while preserving the equilibrium polarization of passive spins.
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A Sensitive HN(CA)CO Experiment for Deuterated Proteins
Journal of Magnetic Resonance, Series B, 1996H, Matsuo, H, Li, G, Wagner
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