Results 231 to 240 of about 137,826 (378)

Evaluation of gene editing in CHO cells using the Cas‐CLOVER system

Biotechnology Progress, EarlyView.
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, Daniel Machado, Victor Cairns, Christine DeMaria, John J. Scarcelli   +13 more
wiley   +1 more source

Whole genome sequencing approach to assess homologous recombination deficiency in a pan-cancer cohort. [PDF]

Commun Med (Lond)
Al Assaad M, Hadi K, Levine MF, Guevara D, Patel M, Tranquille M, King A, Otilano J, Semaan A, Gundem G, Medina-Martínez JS, Sigouros M, Manohar J, Kuo HH, Wilkes DC, Andreopoulou E, Chapman-Davis E, Tagawa ST, Sboner A, Ocean AJ, Shah MA, Papaemmanuil E, Sternberg CN, Holcomb K, Nanus DM, Elemento O, Mosquera JM.   +26 more
europepmc   +1 more source

Systematic Analysis of Homologous Recombination Deficiency Testing in Ovarian Cancer—Development of Recommendations for Optimal Assay Performance [PDF]

Marcel Romey, Fiona R. Rodepeter, Akira Hattesohl, Kristin Kaiser, Julia Teply‐Szymanski, Florian Heitz, Annette Staebler, Violeta Serra, Albert Grass, Frederik Marmé, Kirsten M. Timms, Philipp Harter, Alba Llop‐Guevara, Stefan Kommoss, Jelena Boekhoff, Carsten Denkert   +15 more
openalex   +1 more source

A Bifunctional Cytochrome P450 Enzyme Catalyzes Hydroxylation and Aryl‐Aryl Ether Formation in the Biosynthesis of Emestrin

Chemistry – A European Journal, EarlyView.
Conversion of emestrin J (5) to emestrin (1) by three P450 enzymes from the cluster (eme) in Emericella quadrilineata. EmeO acts as a bifunctional enzyme for the construction of the 15‐membered lactone ring via an aryl‐aryl ether bond formation and simultaneous hydroxylation between phenolic and nonphenolic aromatic rings, while EmeE and EmeR install ...
Yu‐Chuan Chen, Jing‐Jing Wu, Ming‐Hua Chen, Shu‐Ming Li   +3 more
wiley   +1 more source

Accurate tumor purity determination is critical for the analysis of homologous recombination deficiency (HRD)

Translational Oncology, 2023
Michael Menzel, V. Endris, C. Schwab, K. Kluck, O. Neumann, Susanne Beck, M. Ball, C. Schaaf, S. Fröhling, P. Lichtner, P. Schirmacher, Daniel Kazdal, A. Stenzinger, J. Budczies   +13 more
semanticscholar   +1 more source

Carbon Dots for Cancer Theranostics: Synthesis Strategies, Luminescence Properties, and Advances in Bioimaging‐Guided Diagnosis and Therapy

Chemistry – A European Journal, EarlyView.
Lighting up the path to precision oncology: This review comprehensively summarizes the rational design of carbon dots (CDs), elucidating how core size, surface chemistry, and heteroatom doping dictate their luminescence mechanisms. Special emphasis is placed on engineering NIR‐II emissive CDs for deep‐tissue imaging.
Zekun Yan, Yupeng Liu, Jinyun Tan, Songnan Qu   +3 more
wiley   +1 more source

A RAD51 functional assay as a candidate test for homologous recombination deficiency in ovarian cancer.

Gynecologic Oncology, 2023
F. Blanc-Durand, Elisa Yaniz-Galende, A. Llop-Guevara, C. Genestie, V. Serra, A. Herencia-Ropero, C. Klein, D. Berton, A. Lortholary, N. Dohollou, C. Desauw, M. Fabbro, E. Malaurie, Nathalie Bonichon-Lamaichhane, C. Dubot, J. Kurtz, G. de Rauglaudre, N. Raban, A. Chevalier-Place, G. Ferron, M. Kaminsky, C. Kramer, E. Rouleau, A. Leary   +23 more
semanticscholar   +1 more source

Genomic and molecular landscape of DNA damage repair deficiency across The Cancer Genome Atlas [PDF]

, 2018
Bailey, Matthew H, Ding, Li, et al,
core   +1 more source

PEK14: A Kinesin‐4 Necessary for Male‐Derived Fertility in Arabidopsis thaliana

Cytoskeleton, EarlyView.
ABSTRACT Of the 61 kinesins annotated in Arabidopsis thaliana, many are still without assigned function. Here, we have screened an insertional mutant library of Arabidopsis pollen‐expressed kinesins for fertility defects. Insertional mutants for three kinesins showed a significant reduction in seed set.
Isabella N. Mendes, Norman R. Groves, Jessica Z. Li, Lily N. Thompson, Brian J. Smith, Aman Y. Husbands, Iris Meier   +6 more
wiley   +1 more source

Homologous Recombination Deficiency and Survival in Ovarian High-Grade Serous Carcinoma by Self-Reported Race. [PDF]

Cancer Epidemiol Biomarkers Prev
Lawson-Michod KA, Johnson CE, Barnard ME, Davidson NR, Collin LJ, Nix DA, Huff CD, Berchuck A, Salas LA, Greene CS, Marks JR, Peres LC, Doherty JA, Schildkraut JM.   +13 more
europepmc   +1 more source