Results 31 to 40 of about 510 (124)
Purification of HRTV mutant Gc proteins.
, 2023 A. SEC analysis of purified HRTV Gc mutant proteins. HRTV Gc WT and mutants were collected from the same fractions for subsequence binding assay. (TIF)
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Supplementing with GlcCer enhances HRTV infection.
, 2023 A-B. Exogenous glycosphingolipid supplement for HRTV replication in SPTLC2 or UGCG KO 293T cells. Cells were cultured in media supplemented with 0–50 μM indicated glycosphingolipid for 24 hours before HRTV infection (MOI = 1).
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Comparison of Gc protein between HRTV, DBV, and RVFV.
, 2023 A. Diagram of the HRTV M segment. Gc ectodomain is colored by domains (red, domain I; yellow, domain II; blue, domain III). TM, transmembrane domain. B. Sequence alignment of Gc glycoproteins of HRTV, DBV, and RVFV.
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HRTV requires GlcCer for efficient infection.
, 2023 A-B. Effect of SPTLC2-, UGCG- or UGT8-deficiency on HRTV replication. Control and KO 293T cells were infected with HRTV (MOI = 1) for the indicated times for qPCR analysis. Knockout efficiency of SPTLC2-, UGCG- or UGT8 gene was measured by qPCR.
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HRTV Gc protein purification and liposome preparation.
, 2023 A. Size exclusion chromatography (SEC) analysis of purified HRTV Gc. SEC was performed on a HiLoad 16/600 Superdex 200pg column (Cytiva) equilibrated in 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl. The eluate was analyzed for absorbance at 280 nm.
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GlcCer is critical for HRTV-induced membrane fusion.
, 2023 A. Effect of SPTLC2-, UGCG- or UGT8-deficiency on HRTV binding and internalization. For binding assay, control and KO HeLa cells were incubated with HRTV particles at 4°C for 1 hour.
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Glycosphingolipid downstream of GlcCer is not required for HRTV infection.
, 2023 A-B. Effect of B4GALT5-, B4GALT6- or B4GALT5/6 double-deficiency on HRTV replication. Control and KO 293T cells were infected with HRTV (MOI = 1) for 24 hours, followed by qPCR analysis.
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Geographical distribution and pathogenesis of ticks and tick-borne viral diseases
Frontiers in Microbiology, 2023 Ticks are obligatory hematophagous arthropods that harbor and transmit infectious pathogens to humans and animals. Tick species belonging to Amblyomma, Ixodes, Dermacentor, and Hyalomma genera may transmit certain viruses such as Bourbon virus (BRBV ...
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The HRTV Gc protein is associated with GlcCer in the lipid bilayer.
, 2023 A. Induced-fit docking pose of GlcCer with HRTV Gc protein. HRTV Gc ectodomain (PDB: 5YOW) is represented as ribbons and colored by domains (red, domain I; yellow, domain II; blue, domain III).
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Induced-fit docking pose of HRTV Gc protein with head groups of DOPC and GalCer.
, 2023 A. Induced-fit docking pose of DOPC with HRTV Gc protein. The hydrogen bonds and salt bridge between DOPC and Gc are colored with magenta and cyan, respectively. B. 2D representation of docking interaction of GlcCer with the binding site residues of HRTV
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