Results 171 to 180 of about 1,880 (204)

UV‐Guided Isolation of Fungal Metabolites by HSCCC

Journal of Liquid Chromatography & Related Technologies, 2005
Abstract Analytical standardised reversed phase liquid chromatography (RPLC) data can be helpful in finding a suitable solvent combination for isolation of fungal metabolites by high‐speed counter current chromatography. Analysis of the distribution coefficient (K D) of fungal metabolites in a series of biphasic systems consisting of n‐heptane–ethyl ...
Petur W. Dalsgaard   +2 more
openaire   +1 more source

HSCCC Separation of a Stilbene Glucoside from Polygonum Multiflorum

Journal of Liquid Chromatography & Related Technologies, 1998
Abstract High-speed countercurrent chromatography (HSCCC) was applied to the separation and purification of 2,3,5,4′-tetrahydroxy stilbene-2-O-D-glucoside from dried roots (2.7g) of Polygonum multiflorum. The ethanol extract (240 mg) was first separated with the solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 10:1:10 and the
Xueli Cao   +5 more
openaire   +1 more source

One-Step Separation of Four Flavonoids from Herba Salviae Plbeiae by HSCCC

Journal of Chromatographic Science, 2014
High-speed countercurrent chromatography (HSCCC) was successfully applied to the isolation and purification of four flavonoids from Herba Salviae Plbeiae using stepwise and dual-mode elution with a pair of two-phase solvent systems. The systems composed of n-hexane-chloroform-methanol-water (0.5 : 4 : 3 : 2, v/v/v/v) and chloroform-methanol-water (4 ...
Jia, Li   +4 more
openaire   +2 more sources

Preparation and Purification of Epigallocatechin by High‐Speed Countercurrent Chromatography (HSCCC)

Journal of Liquid Chromatography & Related Technologies, 2004
Abstract Epigallocatechin (EGC) was prepared by the degallation of Epigallocatechin‐O‐3‐gallate (EGCG). EGCG was completely converted to EGC and gallic acid by adding 4 mg tannase/100 mg EGCG at the concentration of 2 mg/mL under pH 6.0, at 35°C for 30 min.
Xueli Cao, Yoichiro Ito
openaire   +1 more source

Separation of Flavonoids from the Leaves of Oroxylum indicum by HSCCC

Chromatographia, 2008
A high-speed counter-current chromatography system (HPCCC) capable of rapid processing has been employed to separate seven flavonoids from a methanolic extract of the leaves of Oroxylum indicum by a one-step isocratic elution using a chloroform–methanol–water (9.5:10:5) two-phase system. LC, MS and NMR have identified the components from the extract as
Yuan Yuan   +6 more
openaire   +1 more source

High Speed Countercurrent Chromatography/Fourier Transform Infrared (HSCCC/FT-IR) Spectrometry

SPIE Proceedings, 1985
High Speed Countercurrent Chromatography/Fourier transform infrared (HSCCC/FT-IR) spectrometry is present-ed. High solute-to-solvent ratios can be obtained in separations performed using HSCCC. At these high solute concentrations it is possible to obtain spectra of solutes without solvent elimination, even in the presence of polar solvents, such as ...
Rodolfo J. Romanach, James A. de Haseth
openaire   +1 more source

Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones

Journal of Liquid Chromatography & Related Technologies, 2005
Abstract High speed countercurrent chromatography (HSCCC) was used to isolate the major kavalactones kavain, 7,8‐dihydrokavain, methysticin, 7,8‐dihydromethysticin, yangonin, and demethoxyyangonin. An ethanolic crude extract of kava root was subjected to HSCCC separation. Due to the presence of polymeric material, the sample load was limited.
Katrien Schäfer, Peter Winterhalter
openaire   +1 more source

Preparative HSCCC isolation of phloroglucinolysis products from grape seed polymeric proanthocyanidins as new powerful antioxidants

Food Chemistry, 2015
Polymeric proanthocyanidins isolated from a grape seed phenolic extract were hydrolysed in the presence of phloroglucinol into monomer catechins and their nucleophile derivatives. Each of the phloroglucinolysis products was successfully separated and isolated in large amount by semi-preparative HSCCC technique under the optimized conditions based on a ...
Shuting, Zhang   +6 more
openaire   +2 more sources

Preparation of highly purified oleuropein by combinative technology off line of HSCCC‐PHPLC based on dual wavelength

Journal of Food Science, 2021
Abstract Oleuropein is the main active substance in foods or functional foods produced from olive ( Olea europaea L.) leaves. In the present study, the combinative technology off line of HSCCC‐PHPLC based on dual wavelength was used to separate ...
Ningli Wang   +5 more
openaire   +2 more sources

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