Results 101 to 110 of about 12,913,201 (303)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Designing for Conversational Interaction with Interactive Dance Works [PDF]

, 2011
In this paper we describe ongoing work, which explores the physicality of human-computer interaction in dance works. The use of physical simulations in the interface to connect with the performers and audiences lived experience of the physical world is ...
CLARKSON, D, Johnston, AJ
core  

Multimodal Child-Robot Interaction: Building Social Bonds [PDF]

, 2013
07.01.14 KB. Ok to add published version to spiral, OA paper.For robots to interact effectively with human users they must be capable of coordinated, timely behavior in response to social context.
Cosi, Piero, Belpaeme, TonyTW068020022768090000-0001-5207-7745034899FA-C938-11E5-A139-C70DB5D1D7B1, Demiris, Yiannis, Enescu, Valentin, Neerincx, Mark, Ros-Espinoza, Raquel, Athanasopoulos, Georgios, Hiolle, Antione, Kruijff-Korbayová, Ivana, Belpaeme, Tony, Wood, Rachel, Humbert, Remi, Cuayáhuitl, Heriberto, Cañamero, Lola, Racioppa, Stefania, Read, Robin, Lewis, Matthew, Kiefer, Bernd, Beck, Aryel, Paci, Giulio, Baxter, Paul E, Sommavilla, Giacomo, Baroni, Ilaria, Tesser, Fabio, Nalin, Marco, Looije, Rosemarijn   +25 more
core   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Successful Use of Teams in a Human Computer Interaction REU: Combining Intensive Instruction with Strong Mentoring [PDF]

, 2009
SPIRE-EIT (Summer Program for Interdisciplinary Research and Education – Emerging Interface Technologies) at Iowa State University is a 10-week interdisciplinary summer Research Experience for 15 Undergraduates (NSF-funded) that integrates research and ...
Shill, Pam, Saunders, Kevin, Gilbert, Stephen   +2 more
core  

Personalized Visualization of the Gestures of Parkinson’s Disease Patients with Virtual Reality

Future Internet
Parkinson’s disease is a neurological disorder characterized by motor and non-motor symptoms. Assessment methods, despite the many years of existence of the disease, lack individualized visualization. On the other hand, virtual reality promises immersion
Konstantinos Sakkas, Eirini Georgia Dimitriou, Niki Eleni Ntagka, Nikolaos Giannakeas, Konstantinos Kalafatakis, Alexandros T. Tzallas, Evripidis Glavas   +6 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source