Results 101 to 110 of about 133,158,617 (301)

PCR amplification and genetic analysis in a microwell cell culturing chip

, 2009
We have previously described a microwell chip designed for high throughput, long-term single-cell culturing and clonal analysis in individual wells providing a controlled way of studying high numbers of individual adherent or non-adherent cells.
Ahmadian, Afshin,, Brismar, Hjalmar,, Ahmadian, A, Lindstrom, S, Brismar, H,, Andersson-Svahn, H, Hammond, Maria,, Andersson-Svahn, Helene,, Hammond, M, Lindström, Sara,   +9 more
core   +1 more source

A perfused human blood–brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport

Fluids and Barriers of the CNS, 2018
Receptor-mediated transcytosis is one of the major routes for drug delivery of large molecules into the brain. The aim of this study was to develop a novel model of the human blood–brain barrier (BBB) in a high-throughput microfluidic device.
N. Wevers, Dhanesh G. Kasi, Taylor Gray, K. Wilschut, Benjamin A. Smith, R. van Vught, Fumitaka Shimizu, Yasuteru Sano, T. Kanda, G. Marsh, S. Trietsch, P. Vulto, H. Lanz, B. Obermeier   +13 more
semanticscholar   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

On-a-Chip-Based Sensitive Detection of Drug-Induced Apoptosis in Polarized Gastric Epithelial Cells

, 2021
Microfluidic devices for culturing cells have been successfully utilized for biomedical applications, including drug screening. Several cell lines could be cultivated in microengineered environments with promising results, but gastric cell lines have not
Ulrike Steinmann, Ulrike Steinmann (335615), Liubov Bakhchova, Berend Isermann, Phatcharida Jantaree (3821836), Michael Naumann (87890), Liubov Bakhchova (11628755), Anubhuti Gupta (11628758), Phatcharida Jantaree, Anubhuti Gupta, Berend Isermann (526770), Michael Naumann   +11 more
core   +1 more source

Metastatic niche shaped by host factors influences disseminated cancer cell fate

FEBS Letters, EarlyView.
Metastasis is shaped not only by cancer cells but also by the environments they encounter. This review explores how factors such as aging, diet, the microbiome, lifestyle, and environmental exposures remodel organ‐specific niches in the lung, liver, bone, and brain, influencing where metastatic cells survive, remain dormant, or grow, and ultimately ...
Gwennan Delyth Ward, Álvaro Ruiz‐Tabas, Patricia Altea‐Manzano   +2 more
wiley   +1 more source

Monitoring of Electrophysiological Functions in Brain‐on‐a‐Chip and Brain Organoids

Advanced NanoBiomed Research
Though animal models are still the gold standard for fundamental biological studies and drug evaluation for brain diseases, concerns arise from an apparent lack of reflecting the human genetics and pathophysiology.
Jiyoung Song, Hoon Eui Jeong, Andrew Choi, Hong Nam Kim   +3 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Rapid determination of vitamin B12 concentration with a chemiluminescence lab on a chip [PDF]

, 2012
This paper reports a novel method for the rapid determination of vitamin B12 concentration in a continuous-flow lab-on-a-chip system. This new method is based on luminol-peroxide chemiluminescence (CL) assays for the detection of cobalt(II) ions in ...
Nguyen, Nam-Trung, Muttalib, Siti Zubaidah Binte Abdul, Kwok, Yien Chian, Siti Zubaidah Abdul Muttalib, Lok, Khoi Seng, Lee, Peter Peng Foo   +5 more
core   +1 more source

From mice to clinical relevance: humanizing neuroscience with human-based model systems

Frontiers in Cellular Neuroscience
Preclinical research in neuroscience has traditionally relied on animal models to investigate disease mechanisms and develop new therapeutic strategies.
Aniella Vanessa Bak, Karen M. J. van Loo, Karen M. J. van Loo, Deborah Kronenberg-Versteeg, Deborah Kronenberg-Versteeg, Henner Koch   +5 more
doaj   +1 more source

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source