Results 111 to 120 of about 270,658 (298)

A robotic platform for fluidically-linked human body-on-chips experimentation [PDF]

, 2019
Here we describe of an ‘Interrogator’ instrument that uses liquid-handling robotics, a custom software package, and an integrated mobile microscope to enable automated culture, perfusion, medium addition, fluidic linking, sample collection, and in situ microscopic imaging of up to 10 Organ Chips ...
Novak, Richard, Ingram, Miles, Clauson, Susan, Das, Debarun, Delahanty, Aaron, Herland, Anna, Maoz, Ben M., Jeanty, Sauveur S. F., Somayaji, Mahadevabharath R., Burt, Morgan, Calamari, Elizabeth, Chalkiadaki, Angeliki, Cho, Alexander, Choe, Youngjae, Chou, David Benson, Cronce, Michael, Dauth, Stephanie, Divic, Toni, Fernandez-Alcon, Jose, Ferrante, Thomas, Ferrier, John, FitzGerald, Edward A., Fleming, Rachel, Jalili-Firoozinezhad, Sasan, Grevesse, Thomas, Goss, Josue A., Hamkins-Indik, Tiama, Henry, Olivier, Hinojosa, Chris, Huffstater, Tessa, Jang, Kyung-Jin, Kujala, Ville, Leng, Lian, Mannix, Robert, Milton, Yuka, Nawroth, Janna, Nestor, Bret A., Ng, Carlos F., O’Connor, Blakely, Park, Tae-Eun, Sanchez, Henry, Sliz, Josiah, Sontheimer-Phelps, Alexandra, Swenor, Ben, Thompson II, Guy, Touloumes, George J., Tranchemontagne, Zachary, Wen, Norman, Yadid, Moran, Bahinski, Anthony, Hamilton, Geraldine A., Levner, Daniel, Levy, Oren, Przekwas, Andrzej, Prantil-Baun, Rachelle, Parker, Kevin K., Ingber, Donald E.   +56 more
openaire   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Standard: human intestine-on-a-chip

Cell Regeneration
Organs-on-chips are microphysiological systems that allow to replicate the key functions of human organs and accelerate the innovation in life sciences including disease modeling, drug development, and precision medicine.
Haitao Liu, Yaqing Wang, Xu Zhang, Min Zhang, Peng Wang, Jing Shang, Zhongqiang Li, Likun Gong, Jiabin Guo, Wei Sun, Jingbo Pi, Xianliang Li, Wei Ding, Dianbing Wang, Zhongyu Li, Jingzhong Zhang, Lan Wang, Xingchao Geng, Ruifu Yang, Pingkun Zhou, Wanjin Tang, Xian’en Zhang, Chunying Chen, Shengli Yang, Jianhua Qin   +24 more
doaj   +1 more source

Towards Multi-Organoid Systems for Drug Screening Applications

Bioengineering, 2018
A low percentage of novel drug candidates succeed and reach the end of the drug discovery pipeline, mainly due to poor initial screening and assessment of the effects of the drug and its metabolites over various tissues in the human body.
Cláudia C. Miranda, Tiago G. Fernandes, Maria Margarida Diogo, Joaquim M. S. Cabral   +3 more
doaj   +1 more source

In Vitro Model of Fetal Human Vessel On-chip to Study Developmental Mechanobiology.

Journal of visualized experiments : JoVE, 2023
The heart is the first organ to be functionally established during development, thus initiating blood circulation very early in gestation. Besides transporting oxygen and nutrients to ensure fetal growth, fetal circulation controls many crucial developmental events taking place within the endothelial layer through mechanical cues. Biomechanical signals
Ventura, Telma, Egan, Ewan, Romanò, Nicola, Fidanza, Antonella   +3 more
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Standard: human liver-on-a-chip

Cell Regeneration
Organs-on-chips are microphysiological systems designed to replicate key functions of human organs, thereby accelerating innovation in life sciences, such as disease modeling, drug development, and precision medicine.
Haitao Liu, Xu Zhang, Yaqing Wang, Min Zhang, Peng Wang, Jing Shang, Zhongqiang Li, Likun Gong, Xin Xie, Dongyang Liu, Jingbo Pi, Xinghua Gao, Xianliang Li, Wei Ding, Dianbing Wang, Yun Long, Lan Wang, Song Li, Xingchao Geng, Pingkun Zhou, Wanjin Tang, Xian’en Zhang, Chunying Chen, Shengli Yang, Jianhua Qin   +24 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Organoids and organ‐on‐a‐chip models for investigating the pathophysiology of the human reproductive system

Interdisciplinary Medicine
Organoids and organ‐on‐a‐chips (OoCs), as innovative tools that overcome the limitations of traditional cell and animal models, provide highly biomimetic platforms for studying the pathophysiological mechanisms of the reproductive system.
Hongqi Zhang, Shiwen Deng, Fangfang Jin, Jiayao Qu, Xiang Fan, Peng Chen, Xinrong Fan   +6 more
doaj   +1 more source