Results 271 to 280 of about 119,573 (312)

Insulin receptors in cultured human keratinocytes

British Journal of Dermatology, 1984
Insulin binding to receptors was studied for the first time in cultured normal epidermal human keratinocytes. Binding was measured at 16 degrees C in steady-state conditions using 125I-labelled iodohormone and varying concentrations of unlabelled hormone.
P, Verrando, J P, Ortonne
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In-vitro cultivation of human oral keratinocytes

Archives of Oral Biology, 1979
Abstract The technique of Rheinwald and Green (1975b) for the serial cultivation of foreskin keratinocytes was modified to facilitate the cultivation of gingival and buccal keratinocytes. We used 7–10-day epithelial outgrowths from excised tissue fragments as a source of keratinocytes, whereas Rheinwald and Green used enzymically-dissociated ...
L, Taichman, S, Reilly, P R, Garant
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Human keratinocytes express fractalkine/CX3CL1

Journal of Dermatological Science, 2003
fractalkine/CX3CL1 is a unique chemokine that has properties of both chemoattractants and adhesion molecules. The major source of this chemokine in the skin is still controversial.studies were undertaken to determine the expression of fractalkine in human skin.RT-PCR, Western blotting, and immunostaining were performed with normal human epidermal ...
Makoto, Sugaya, Koichiro, Nakamura, Hiroshi, Mitsui, Tomonori, Takekoshi, Hidehisa, Saeki, Kunihiko, Tamaki   +5 more
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Clonal analysis of human clonogenic keratinocytes

2022
Regenerative medicine has its roots in harnessing stem cells for permanent restoration of damaged or diseased tissues. The first procedure for the transplantation of epidermal cultures in massive full-thickness burns was established in the 1980s. Since then, epithelial stem cell-based therapies have been further developed in cell and gene therapy ...
Enzo E., Cattaneo C., Consiglio F., Polito M. P., Bondanza S., De Luca M.   +5 more
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Effect of strain on human keratinocytes in vitro

Journal of Cellular Physiology, 1997
Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsible for these changes.
T, Takei, C, Rivas-Gotz, C A, Delling, J T, Koo, I, Mills, T L, McCarthy, M, Centrella, B E, Sumpio   +7 more
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Tissue culture of human cholesteatomatous keratinocytes

Clinical Otolaryngology, 1983
The aim of this study was to culture keratinocytes from migratory skin and from cholesteatomatous matrix. At operation, cholesteatomatous matrix, normal drumhead and non-migratory stratifying skin from the extrameatal incision were obtained. Successful cultures of keratinocytes were produced in 4 out of 5 attempts.
D W, Proops, E K, Parkinson
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Keratins in Cultivated Human Keratinocytes Are Stable*

2015
Cultures of human keratinocytes were established according to the technique of Rheinwald and Green. When cultures were exposed to [14C] leucine, the uptake of leucine and increase in the specific activity of 6 urea-extractable polypeptides was prompt-each keratin achieved 50% of its peak specific activity in 3-6 hours.
J, McGuire, L, Milstone, M, Osber, L, Ingalls   +3 more
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Captopril modulates acetylcholinesterase in human keratinocytes

Archives of Dermatological Research, 2011
Human keratinocytes synthesize and secrete non-neuronal acetylcholine, which acts as a local cell signaling molecule, regulating functions like proliferation, cell adhesion, motility, desmosomal cell contact, and glandular activity. The keratinocyte acetylcholine axis is composed of the enzymes mediating acetylcholine synthesis (acetyltransferase) and ...
Baroni, A, BUOMMINO, ELISABETTA, Ruocco, E, Petrazzuolo, M, DE FILIPPIS, A, Satriano, R, Ruocco, V, Cozza, V, Tufano, M.   +8 more
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CD40 is functionally expressed on human keratinocytes

European Journal of Immunology, 1996
AbstractThe CD40/gp39 pathway is known to be an important feature of B/T cell collaboration leading to T cell‐dependent activation, proliferation or differentiation of B cells. Additionally, CD40 is involved in the regulation of B cell survival and apoptosis.
Denfeld, Ralf W., Hollenbaugh, Diane, Fehrenbach, Alexandra, Weiss, Johannes, Leoprechting, Achim von, Mai, Brigit, Voith, Ursula, Schöpf, Erwin, Aruffo, Alejandro A., Simon, Jan C.   +9 more
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Chloride Transport Pathways in Human Keratinocytes

1991
The transport of C1- across cell membrane plays an important role in a number of biological functions, including the maintenance of cell volume and the regulation of intracellular pH. A variety of systems capable of transporting C1- across the plasma membrane have been described in mammalian cells.
M, Rugolo, M, De Luca, T, Mastrocola
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