Results 71 to 80 of about 365,728 (264)

Variability of human pluripotent stem cell lines [PDF]

Current Opinion in Genetics & Development, 2017
Human pluripotent stem cells derived from embryos (human Embryonic Stem Cells or hESCs) or generated by direct reprogramming of somatic cells (human Induced Pluripotent Stem Cells or hiPSCs) can proliferate almost indefinitely in vitro while maintaining the capacity to differentiate into a broad diversity of cell types.
Ortmann, Daniel, Vallier, Ludovic
openaire   +3 more sources

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Genome Editing in Human Pluripotent Stem Cells [PDF]

Cold Spring Harbor Protocols, 2016
Pluripotent stem cells (PSCs), defined by their capacity for self-renewal and differentiation into all cell types, are an integral tool for basic biological research and disease modeling. However, full use of PSCs for research and regenerative medicine requires the ability to precisely edit their DNA to correct disease-causing mutations and for ...
Cory, Smith, Zhaohui, Ye, Linzhao, Cheng
openaire   +2 more sources

Transfer Printing and Reconfiguration of Soft Electronics Using Digital Microfluidics and Laser Machining

Advanced Functional Materials, EarlyView.
This paper presents a digital microfluidics‐based technique for transferring and reconfiguring soft nanomembranes. Laser‐machined nanothin membranes are picked up, transported, and aligned via tailored surface tension and the actuation of water droplets, enabling the development of flexible electronics, the integration of functional materials on 3D ...
Quang Anh Nguyen, Chi Cong Nguyen, Tran Bach Dang, Phuoc Thien Phan, Jordan Thorpe, Michael Listyawan, Nhat Minh Doan, Sinuo Zhao, Hongru Chen, Trung Thien Hoang, Mingsheng Xu, Jisheng Han, Thanh Nho Do, Adam Hill, Timothée Mouterde, Hoang‐Phuong Phan   +15 more
wiley   +1 more source

Organoid-induced differentiation of conventional T cells from human pluripotent stem cells

Cell Stem Cell, 2019
Summary The ability to generate T cells from self-renewing pluripotent stem cells (PSC) has the potential to transform the current practice of autologous T cell immunotherapy into universal off-the-shelf products.
A. Montel-Hagen, C. Seet, Suwen Li, Brent Chick, Yuhua Zhu, Patrick Chang, Steven C Tsai, Victoria Sun, Shawn A. Lopez, Ho-Chung Chen, Chongbin He, C. J. Chin, D. Casero, G. Crooks   +13 more
semanticscholar   +1 more source

Microfabricated Anisotropic Myobundles for the Scalable Production of Cardiac Tissue Grafts

Advanced Functional Materials, EarlyView.
Controlling the anisotropy of cardiac tissue remains an outstanding challenge in the field of cardiac tissue engineering. Here, we introduce an approach to generate anisotropic cardiac myobundles using cell‐adhesive, synthetic, electrospun fibers and stem cell‐derived cardiac fibroblasts.
Maggie E. Jewett, Javiera Jilberto, Margaret E. Stanley, David O. Ntim, Siddhi M. Bhirud, Avianna T. Schroeder, Gonzola X. Anyosa Galvez, Jingyi Xia, Samuel J. DePalma, Emmanouil Agrafiotis, Susan S. Xi, Amanda S. Bluem, Courtney M. Lestock, Adam S. Helms, David A. Nordsletten, Brendon M. Baker   +15 more
wiley   +1 more source

Multifunctional Gold Nanocluster‐Based PROTAC System for Targeted Degradation of Phosphorylated Tau and Modulation of Neuroinflammation in Alzheimer's Disease

Advanced Functional Materials, EarlyView.
We present a novel proteolysis‐targeting chimera (PROTAC) system conjugated to lipoic acid gold nanoclusters (PLANC), designed to degrade pTau, regulate inflammatory signaling, and effectively traverse the blood‐brain barrier (BBB). PLANC degraded pTau at various phosphorylation sites, with mechanistic studies confirming proteasome‐mediated degradation
Sarah Nevins, Callan D. McLoughlin, Wan‐Kyu Ko, Hye Kyu Choi, Brandon Conklin, Joshua B. Stein, Yannan Hou, Hongwon Kim, Rohan Khulbe, Ki‐Bum Lee   +9 more
wiley   +1 more source

Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis.

Cell Stem Cell, 2018
The development of complex in vitro hepatic systems and artificial liver devices has been hampered by the lack of reliable sources for relevant cell types, such as hepatic stellate cells (HSCs).
M. Coll, L. Perea, R. Boon, S. Leite, Julia Vallverdú, I. Mannaerts, Ayla Smout, A. El Taghdouini, Delia Blaya, Daniel Rodrigo-Torres, I. Graupera, Beatriz Aguilar‐Bravo, C. Chesné, M. Najimi, E. Sokal, J. Lozano, L. V. van Grunsven, C. Verfaillie, P. Sancho-Bru   +18 more
semanticscholar   +1 more source

Prevention of tumor risk associated with the reprogramming of human pluripotent stem cells

Journal of experimental & clinical cancer research : CR, 2020
Human pluripotent embryonic stem cells have two special features: self-renewal and pluripotency. It is important to understand the properties of pluripotent stem cells and reprogrammed stem cells.
Kenly Wuputra, Chia-Chen Ku, Deng-Chyang Wu, Ying-Chu Lin, Shigeo Saito, K. Yokoyama   +5 more
semanticscholar   +1 more source

Mechanical and Electrical Phenotype of hiPSC‐Cardiomyocytes on Fibronectin‐Based Hydrogels

Advanced Healthcare Materials, EarlyView.
We introduce fibronectin‐based PEG hydrogels with controlled rigidity to enable the culture of iPSC‐derived cardiomyocytes. These substrates offer an alternative to the current culture of these cells on fibronectin‐coated glass, providing enhanced structural and functional behavior. The system provides a more physiologically relevant platform to assess
Ana Da Silva Costa, Lineta Stonkute, Sara Trujillo, Mariana Azevedo Gonzalez Oliva, Francis Burton, Matthew J. Dalby, Oana Dobre, Godfrey Smith, Manuel Salmeron‐Sanchez   +8 more
wiley   +1 more source