Results 51 to 60 of about 126,711 (266)

Modeling human infertility with pluripotent stem cells [PDF]

Stem Cell Research, 2017
Human fertility is dependent upon the correct establishment and differentiation of the germline. This is because no other cell type in the body is capable of passing a genome and epigenome from parent to child. Terminally differentiated germline cells in the adult testis and ovary are called gametes. However, the initial specification of germline cells
Chen, Di, Gell, Joanna J, Tao, Yu, Sosa, Enrique, Clark, Amander T   +4 more
openaire   +4 more sources

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin

Regenerative Therapy, 2020
Introduction: Tumourigenesis attributed to residual undifferentiated cells in a graft is considered to be a significant issue in cell therapy using human pluripotent stem cells.
Yoshikazu Haramoto, Yasuko Onuma, Shuuji Mawaribuchi, Yoshiro Nakajima, Yasuhiko Aiki, Kumiko Higuchi, Madoka Shimizu, Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito   +9 more
doaj   +1 more source

Immunocytochemical Analysis of Human Pluripotent Stem Cells [PDF]

, 2011
This chapter will describe the most common immunocytochemical method utilized in the stem cell field - using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody's isotype.
Hubert E, Nethercott, David J, Brick, Philip H, Schwartz   +2 more
openaire   +2 more sources

Biobanking of human pluripotent stem cells in China

Cell Proliferation, 2022
AbstractIn recent years, significant progress has been made internationally in the development of human pluripotent stem cell (hPSC)‐derived products for serious and widespread disorders. Biobanking of the cellular starting materials is a crucial component in the delivery of safe and regulatory compliant cell therapies.
Glyn Nigel Stacey, Jie Hao
openaire   +2 more sources

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

Energy metabolism in human pluripotent stem cells and their differentiated counterparts. [PDF]

PLoS ONE, 2011
Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies.
Sandra Varum, Ana S Rodrigues, Michelle B Moura, Olga Momcilovic, Charles A Easley, João Ramalho-Santos, Bennett Van Houten, Gerald Schatten   +7 more
doaj   +1 more source

The Tumorigenic Potential of Human Pluripotent Stem Cells

Stem Cells Translational Medicine, 2022
Abstract Human pluripotent stem cells (hPSCs) are currently evaluated for clinical applications due to their proliferation and differentiation capacities, raising the need to both assess and enhance, the safety of hPSC-based treatments. Distinct molecular features contribute to the tumorigenicity of hPSCs, manifested in the formation of ...
Elyad Lezmi, Nissim Benvenisty
openaire   +2 more sources

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Trehalose to cryopreserve human pluripotent stem cells

Stem Cell Research, 2018
The successful exploitation of human pluripotent stem cells (hPSCs) for research, translational or commercial reasons requires the implementation of a simple and efficient cryopreservation method. Cryopreservation is usually performed with dimethylsulphoxide (DMSO), in addition to animal proteins.
Aikaterini Ntai, Alberto La Spada, Pasquale De Blasio, Ida Biunno   +3 more
openaire   +4 more sources