Results 41 to 50 of about 365,728 (264)

Generation of lung organoids from human pluripotent stem cells in vitro

Nature Protocols, 2019
The lung epithelium is derived from the endodermal germ layer, which undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate the final arborized network of conducting airways (bronchi, bronchioles) and gas-exchanging units ...
Alyssa J Miller, Briana R. Dye, Daysha Ferrer-Torres, David R. Hill, Arend W. Overeem, L. Shea, J. Spence   +6 more
semanticscholar   +1 more source

Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions

Nature Communications, 2020
The occurrence of repetitive genomic changes that provide a selective growth advantage in pluripotent stem cells is of concern for their clinical application. However, the effect of different culture conditions on the underlying mutation rate is unknown.
O. Thompson, Ferdinand von Meyenn, Z. Hewitt, J. Alexander, A. Wood, R. Weightman, Sian Gregory, Felix Krueger, S. Andrews, Ivana Barbaric, P. Gokhale, H. Moore, W. Reik, M. Milo, S. Nik-Zainal, K. Yusa, P. Andrews   +16 more
semanticscholar   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

The Decision on the “Optimal” Human Pluripotent Stem Cell

Stem Cells Translational Medicine, 2014
Summary Because of recent advances, the array of human pluripotent stem cells now contains embryonic stem cells, derived from “surplus” in vitro fertilization embryos or from cloned embryos; induced pluripotent stem cells; and amniotic fluid stem cells.
Margit, Rosner, Katharina, Schipany, Markus, Hengstschläger   +2 more
openaire   +2 more sources

Calcium signaling in human pluripotent stem cells [PDF]

Cell Calcium, 2016
Human pluripotent stem cells provide new tools for developmental and pharmacological studies as well as for regenerative medicine applications. Calcium homeostasis and ligand-dependent calcium signaling are key components of major cellular responses, including cell proliferation, differentiation or apoptosis.
Apáti, Ágota, Berecz, Tünde, Sarkadi, Balázs   +2 more
openaire   +3 more sources

Transcriptionally Active HERV-H Retrotransposons Demarcate Topologically Associating Domains in Human Pluripotent Stem Cells

Nature Genetics, 2019
Chromatin architecture has been implicated in cell type-specific gene regulatory programs, yet how chromatin remodels during development remains to be fully elucidated.
Yanxiao Zhang, Ting Li, Sebastian Preissl, Maria Luisa Amaral, Jonathan D. Grinstein, Elie N. Farah, Eugin Destici, Yunjiang Qiu, Rong Hu, A. Lee, Sora Chee, Kaiyue Ma, Z. Ye, Quan-sheng Zhu, Hui Huang, Rongxin Fang, Leqian Yu, J. I. Izpisúa Belmonte, Jun Wu, S. Evans, N. Chi, B. Ren   +21 more
semanticscholar   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome

Proceedings of the Japan Academy. Series B, Physical and biological sciences, 2009
Induced pluripotent stem cells (iPSC) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV),
N. Fusaki, H. Ban, Akiyo Nishiyama, Koichi Saeki, M. Hasegawa   +4 more
semanticscholar   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Modeling human infertility with pluripotent stem cells [PDF]

Stem Cell Research, 2017
Human fertility is dependent upon the correct establishment and differentiation of the germline. This is because no other cell type in the body is capable of passing a genome and epigenome from parent to child. Terminally differentiated germline cells in the adult testis and ovary are called gametes. However, the initial specification of germline cells
Chen, Di, Gell, Joanna J, Tao, Yu, Sosa, Enrique, Clark, Amander T   +4 more
openaire   +4 more sources