Results 31 to 40 of about 365,728 (264)

Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

Nature Biotechnology, 2020
Cortico-striatal projections are critical components of forebrain circuitry that regulate motivated behaviors. To enable the study of the human cortico-striatal pathway and how its dysfunction leads to neuropsychiatric disease, we developed a method to ...
Yuki Miura, Min-Yin Li, Fikri Birey, Kazuya Ikeda, Omer Revah, M. Thete, Jin-Young Park, Alyssa Puno, Samuel H. Lee, M. Porteus, S. Pașca   +10 more
semanticscholar   +1 more source

Cardiac differentiation of human pluripotent stem cells [PDF]

Journal of Cellular and Molecular Medicine, 2012
AbstractDue to the extremely limited proliferative capacity of adult cardiomyocytes, human embryonic (pluripotent) stem cell derived cardiomyocytes (hESC‐CMs) are currently almost the only reliable source of human heart cells which are suited to large‐scale production.
Jiang, Junjie, Han, Pengcheng, Zhang, Qiangzhe, Zhao, Jianmin, Ma, Yue, Mercola, Mark   +5 more
openaire   +2 more sources

Nephron organoids derived from human pluripotent stem cells model kidney development and injury

Nature Biotechnology, 2015
Kidney cells and tissues derived from human pluripotent stem cells (hPSCs) may enable organ regeneration, disease modeling and drug screening. We report an efficient, chemically defined protocol for differentiating hPSCs into multipotent nephron ...
MD PhD Ryuji Morizane, MD Albert Q. Lam, PhD Benjamin S. Freedman, PhD Seiji Kishi MD, PhD M. Todd Valerius, M. P. Joseph V. Bonventre   +5 more
semanticscholar   +1 more source

Midbrain-like organoids from human pluripotent stem cells contain functional dopaminergic and neuromelanin producing neurons

Cell Stem Cell, 2016
SUMMARY Recent advances in three dimensional (3D) culture systems have led to the generation of brain organoids that resemble different human brain regions; however, a 3D organoid model of the midbrain containing functional midbrain dopaminergic (mDA ...
J. Jo, Yixin Xiao, A. Sun, Engin Cukuroglu, Hoang-Dai Tran, Jonathan Göke, Zi-Ying Tan, T. Y. Saw, Cheng-Peow Tan, Hidayat Lokman, Young-Hwan Lee, Donghoon Kim, H. Ko, Seong-Oh Kim, J. H. Park, Nam‐Joon Cho, T. Hyde, J. Kleinman, J. Shin, D. Weinberger, E. Tan, H. S. Je, H. Ng   +22 more
semanticscholar   +1 more source

Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells

bioRxiv, 2020
The derivation of self-renewing tissue-specific stem cells from human induced pluripotent stem cells (iPSCs) would shorten the time needed to engineer mature cell types in vitro and would have broad reaching implications for the field of regenerative ...
F. Hawkins, Shingo Suzuki, M. Beermann, Cristina Barillà, Ruobing Wang, C. Villacorta-Martin, A. Berical, J. Jean, Jake Le Suer, T. Matte, C. Simone-Roach, Yang Tang, Thorsten M. Schlaeger, Thorsten M. Schlaeger, A. Crane, Nadine Matthias, Sarah X. L. Huang, S. Randell, Joshua H. Wu, Jason R. Spence, Gianni Carraro, B. Stripp, A. Rab, Eric J. Sorsher, A. Horani, S. Brody, B. Davis, D. N. Kotton   +27 more
semanticscholar   +1 more source

Immunocytochemical Analysis of Human Pluripotent Stem Cells [PDF]

, 2011
This chapter will describe the most common immunocytochemical method utilized in the stem cell field - using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody's isotype.
Hubert E, Nethercott, David J, Brick, Philip H, Schwartz   +2 more
openaire   +2 more sources

RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells

Nature Genetics, 2020
Many chromatin-binding proteins and protein complexes that regulate transcription also bind RNA. One of these, Polycomb repressive complex 2 (PRC2), deposits the H3K27me3 mark of facultative heterochromatin and is required for stem cell differentiation ...
Yicheng Long, Taeyoung Hwang, A. R. Gooding, Karen J. Goodrich, J. Rinn, T. Cech   +5 more
semanticscholar   +1 more source

Advances in mesenchymal stem cell therapy for immune and inflammatory diseases: Use of cell‐free products and human pluripotent stem cell‐derived mesenchymal stem cells

Stem Cells Translational Medicine, 2021
Mesenchymal stem cell therapy (MSCT) for immune and inflammatory diseases continues to be popular based on progressive accumulation of preclinical mechanistic evidence.
Li-Tzu Wang, Ko-Jiunn Liu, H. Sytwu, M. Yen, B. Yen   +4 more
semanticscholar   +1 more source

Trehalose to cryopreserve human pluripotent stem cells

Stem Cell Research, 2018
The successful exploitation of human pluripotent stem cells (hPSCs) for research, translational or commercial reasons requires the implementation of a simple and efficient cryopreservation method. Cryopreservation is usually performed with dimethylsulphoxide (DMSO), in addition to animal proteins.
Aikaterini Ntai, Alberto La Spada, Pasquale De Blasio, Ida Biunno   +3 more
openaire   +4 more sources

HLA Engineering of Human Pluripotent Stem Cells [PDF]

Molecular Therapy, 2013
The clinical use of human pluripotent stem cells and their derivatives is limited by the rejection of transplanted cells due to differences in their human leukocyte antigen (HLA) genes. This has led to the proposed use of histocompatible, patient-specific stem cells; however, the preparation of many different stem cell lines for clinical use is a ...
Riolobos, Laura, Hirata, Roli K, Turtle, Cameron J, Wang, Pei-Rong, Gornalusse, German G, Zavajlevski, Maja, Riddell, Stanley R, Russell, David W   +7 more
openaire   +2 more sources