Results 31 to 40 of about 216,145 (304)

Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells. [PDF]

, 2016
Urea cycle disorders are incurable enzymopathies that affect nitrogen metabolism and typically lead to hyperammonemia. Arginase deficiency results from a mutation in Arg1, the enzyme regulating the final step of ureagenesis and typically results in ...
Angarita, Stephanie Ak, Byrne, James A, Cederbaum, Stephen D, Chang, Katherine M, Gilmore, W Blake, Hermann, Kip, Lam, Alex K, Lee, Patrick C, Lipshutz, Gerald S, Pyle, April D, Schoenberg, Benjamen E, Tang, Jonathan K, Truong, Brian, Vega-Crespo, Agustin, Wininger, Austin E   +14 more
core   +3 more sources

Cardiac differentiation of human pluripotent stem cells [PDF]

Journal of Cellular and Molecular Medicine, 2012
AbstractDue to the extremely limited proliferative capacity of adult cardiomyocytes, human embryonic (pluripotent) stem cell derived cardiomyocytes (hESC‐CMs) are currently almost the only reliable source of human heart cells which are suited to large‐scale production.
Jiang, Junjie, Han, Pengcheng, Zhang, Qiangzhe, Zhao, Jianmin, Ma, Yue, Mercola, Mark   +5 more
openaire   +2 more sources

Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

Scientific Reports, 2017
Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human ...
Kazuhiro Ikeda, Shogo Nagata, Teru Okitsu, Shoji Takeuchi   +3 more
doaj   +1 more source

Generation of hypoimmunogenic human pluripotent stem cells [PDF]

Proceedings of the National Academy of Sciences, 2019
Significance To enable cell therapy on a broader scale, the development of universal donor stem cell products that can be administered to multiple patients in need has been proposed, yet a strategy controlling both adaptive and innate immune rejection has not been reported.
Xiao, Han, Mengning, Wang, Songwei, Duan, Paul J, Franco, Jennifer Hyoje-Ryu, Kenty, Preston, Hedrick, Yulei, Xia, Alana, Allen, Leonardo M R, Ferreira, Jack L, Strominger, Douglas A, Melton, Torsten B, Meissner, Chad A, Cowan   +12 more
openaire   +2 more sources

Telomere dynamics in human pluripotent stem cells

Cell Cycle, 2023
Pluripotent stem cells (PSCs) are a promising source of stem cells for regenerative therapies. Stem cell function depends on telomere maintenance mechanisms that provide them with the proliferative capacity and genome stability necessary to multiply and regenerate tissues.
Ma, Buyun, Martínez, Paula, Sánchez-Vázquez, Raúl, Blasco, Maria A.   +3 more
openaire   +2 more sources

Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. [PDF]

PLoS ONE, 2017
Oligodendrocytes myelinate axons and form myelin sheaths in the central nervous system. The development of therapies for demyelinating diseases, including multiple sclerosis and leukodystrophies, is a challenge because the pathogenic mechanisms of ...
Tomoko Yamashita, Yuki Miyamoto, Yoshio Bando, Takashi Ono, Sakurako Kobayashi, Ayano Doi, Toshihiro Araki, Yosuke Kato, Takayuki Shirakawa, Yutaka Suzuki, Junji Yamauchi, Shigetaka Yoshida, Naoya Sato   +12 more
doaj   +1 more source

Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]

, 2018
The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core   +2 more sources

Modeling human infertility with pluripotent stem cells [PDF]

Stem Cell Research, 2017
Human fertility is dependent upon the correct establishment and differentiation of the germline. This is because no other cell type in the body is capable of passing a genome and epigenome from parent to child. Terminally differentiated germline cells in the adult testis and ovary are called gametes. However, the initial specification of germline cells
Chen, Di, Gell, Joanna J, Tao, Yu, Sosa, Enrique, Clark, Amander T   +4 more
openaire   +4 more sources

GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells

Stem Cell Research & Therapy, 2019
Background Human mesenchymal stem cells are a strong candidate for cell therapies owing to their regenerative potential, paracrine regulatory effects, and immunomodulatory activity.
Madison McGrath, Edmund Tam, Martina Sladkova, Athbah AlManaie, Matthew Zimmer, Giuseppe Maria de Peppo   +5 more
doaj   +1 more source

Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

SAGE Open Medicine, 2020
Objectives: To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency.
Estela Cruvinel, Isabella Ogusuku, Rosanna Cerioni, Sirlene Rodrigues, Jéssica Gonçalves, Maria Elisa Góes, Juliana Morais Alvim, Anderson Carlos Silva, Vanesca de Souza Lino, Enrique Boccardo, Ernesto Goulart, Alexandre Pereira, Rafael Dariolli, Marcos Valadares, Diogo Biagi   +14 more
doaj   +1 more source