Results 31 to 40 of about 123,070 (267)

Immunocytochemical Analysis of Human Pluripotent Stem Cells [PDF]

, 2011
This chapter will describe the most common immunocytochemical method utilized in the stem cell field - using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody's isotype.
Hubert E, Nethercott, David J, Brick, Philip H, Schwartz   +2 more
openaire   +2 more sources

Modelling Human Disease with Pluripotent Stem Cells [PDF]

Current Gene Therapy, 2013
Recent progress in the field of cellular reprogramming has opened up the doors to a new era of disease modelling, as pluripotent stem cells representing a myriad of genetic diseases can now be produced from patient tissue. These cells can be expanded and differentiated to produce a potentially limitless supply of the affected cell type, which can then ...
Richard, Siller, Sebastian, Greenhough, In-Hyun, Park, Gareth J, Sullivan   +3 more
openaire   +2 more sources

Generation of hiPSC lines with fusion tagged thyroid hormone receptor α isoforms to study isoform-specific actions of TRα1 and TRα2

Stem Cell Research
The role of the nuclear thyroid hormone receptor TRα1 as a mediator of thyroid hormone action on target gene expression is well understood. However, the function of the TRα2 splicing isoform, which does not bind thyroid hormones, remains unexplored.
Nina Härting, Katarzyna A. Ludwik, Regina Jahn, Frank J. Kaiser, Harald Stachelscheid   +4 more
doaj   +1 more source

A novel -mutated human induced pluripotent stem cell model for understanding -mutated tumors

Tumor Biology, 2020
A missense mutation of the guanine nucleotide binding protein alpha stimulating activity polypeptide 1 ( GNAS ) gene, typically Arg201Cys or Arg201His (R201H/R201C), leads to constitutive activation of the Gsα-cyclic AMP (cAMP) signaling pathway that ...
Katsuhito Watanabe, Takashi Nakamura, Shoko Onodera, Akiko Saito, Takahiko Shibahara, Toshifumi Azuma   +5 more
doaj   +1 more source

Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. [PDF]

PLoS ONE, 2017
Oligodendrocytes myelinate axons and form myelin sheaths in the central nervous system. The development of therapies for demyelinating diseases, including multiple sclerosis and leukodystrophies, is a challenge because the pathogenic mechanisms of ...
Tomoko Yamashita, Yuki Miyamoto, Yoshio Bando, Takashi Ono, Sakurako Kobayashi, Ayano Doi, Toshihiro Araki, Yosuke Kato, Takayuki Shirakawa, Yutaka Suzuki, Junji Yamauchi, Shigetaka Yoshida, Naoya Sato   +12 more
doaj   +1 more source

Vascular Potential of Human Pluripotent Stem Cells [PDF]

Arteriosclerosis, Thrombosis, and Vascular Biology, 2010
Cardiovascular disease is the number one cause of death and disability in the US. Understanding the biological activity of stem and progenitor cells, and their ability to contribute to the repair, regeneration and remodeling of the heart and blood vessels affected by pathological processes is an essential part of the paradigm in enabling us to achieve ...
Ionela, Iacobas, Archana, Vats, Karen K, Hirschi   +2 more
openaire   +2 more sources

Heart regeneration with human pluripotent stem cells: Prospects and challenges

Bioactive Materials, 2020
Cardiovascular disease, ranging from congenital heart disease to adult myocardial infarction, is the leading cause of death worldwide. In pursuit of reliable cardiovascular regenerative medicine, human pluripotent stem cells (hPSCs), including human ...
Yuqian Jiang, Xiaojun Lance Lian
doaj   +1 more source

Defining differentially methylated regions specific for the acquisition of pluripotency and maintenance in human pluripotent stem cells via microarray. [PDF]

PLoS ONE, 2014
Epigenetic regulation is critical for the maintenance of human pluripotent stem cells. It has been shown that pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells, appear to have a hypermethylated status compared with ...
WenYin He, XiangJin Kang, HongZi Du, Bing Song, ZhenYu Lu, Yuling Huang, Ding Wang, Xiaofang Sun, Yang Yu, Yong Fan   +9 more
doaj   +1 more source

Epigenetic aberrations in human pluripotent stem cells [PDF]

The EMBO Journal, 2019
Human pluripotent stem cells (hPSCs) are being increasingly utilized worldwide in investigating human development, and modeling and discovering therapies for a wide range of diseases as well as a source for cellular therapy. Yet, since the first isolation of human embryonic stem cells (hESCs) 20 years ago, followed by the successful reprogramming of ...
Shiran Bar, Nissim Benvenisty
openaire   +2 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source