Results 11 to 20 of about 216,145 (304)

A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture. [PDF]

PLoS ONE, 2015
Fibroblast growth factors (FGFs) are essential for maintaining self-renewal in human embryonic stem cells and induced pluripotent stem cells. Recombinant basic FGF (bFGF or FGF2) is conventionally used to culture pluripotent stem cells; however, because ...
Yasuko Onuma, Kumiko Higuchi, Yasuhiko Aiki, Yujing Shu, Masahiro Asada, Makoto Asashima, Masashi Suzuki, Toru Imamura, Yuzuru Ito   +8 more
doaj   +1 more source

Single cell heterogeneity in human pluripotent stem cells

BMB Reports, 2021
Human pluripotent stem cells (hPSCs) include human embryonic stem cells (hESCs) derived from blastocysts and human induced pluripotent stem cells (hiPSCs) generated from somatic cell reprogramming. Due to their self-renewal ability and pluripotent differentiation potential, hPSCs serve as an excellent experimental platform for human development ...
Seungbok Yang, Yoonjae Cho, Jiwon Jang
openaire   +4 more sources

Young at Heart: Pioneering Approaches to Model Nonischaemic Cardiomyopathy with Induced Pluripotent Stem Cells [PDF]

, 2016
A mere 9 years have passed since the revolutionary report describing the derivation of induced pluripotent stem cells from human fibroblasts and the first in-patient translational use of cells obtained from these stem cells has already been achieved ...
Gowran, Aoife, Nigro, Patrizia, Perrucci, Gianluca L., Pompilio, Giulio, Rasponi, Marco, Righetti, Stefano, Visone, Roberta, Zanobini, Marco   +7 more
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Brainstem Organoids From Human Pluripotent Stem Cells [PDF]

Frontiers in Neuroscience, 2020
The brainstem is a posterior region of the brain, composed of three parts, midbrain, pons, and medulla oblongata. It is critical in controlling heartbeat, blood pressure, and respiration, all of which are life-sustaining functions, and therefore, damages to or disorders of the brainstem can be lethal. Brain organoids derived from human pluripotent stem
Nobuyuki Eura, Takeshi K. Matsui, Takeshi K. Matsui, Joachim Luginbühl, Masaya Matsubayashi, Hitoki Nanaura, Hitoki Nanaura, Tomo Shiota, Kaoru Kinugawa, Naohiko Iguchi, Takao Kiriyama, Canbin Zheng, Tsukasa Kouno, Yan Jun Lan, Pornparn Kongpracha, Pattama Wiriyasermkul, Yoshihiko M. Sakaguchi, Riko Nagata, Tomoya Komeda, Naritaka Morikawa, Fumika Kitayoshi, Miyong Jong, Shinko Kobashigawa, Mari Nakanishi, Masatoshi Hasegawa, Yasuhiko Saito, Takashi Shiromizu, Yuhei Nishimura, Takahiko Kasai, Maiko Takeda, Hiroshi Kobayashi, Yusuke Inagaki, Yasuhito Tanaka, Manabu Makinodan, Toshifumi Kishimoto, Hiroki Kuniyasu, Shushi Nagamori, Alysson R. Muotri, Alysson R. Muotri, Jay W. Shin, Kazuma Sugie, Eiichiro Mori   +41 more
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Human Amniocytes Are Receptive to Chemically Induced Reprogramming to Pluripotency [PDF]

, 2017
Restoring pluripotency using chemical compounds alone would be a major step forward in developing clinical-grade pluripotent stem cells, but this has not yet been reported in human cells.
Adjaye, J, Campanella, M, De Coppi, P, Eaton, S, Faccenda, D, Guillot, P V, Hau, K-L, Hawkins, K E, Martin-Trujillo, A, Monk, D, Moschidou, D, Ranzoni, A M, Sanchez-Freire, V, Thrasher, A J, Tommasini, F, Wruck, W   +15 more
core   +4 more sources

Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy

Stem Cell Research & Therapy, 2017
Background Human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, hold enormous promise for many biomedical applications, such as regenerative medicine, drug testing, and disease modeling.
Elvira Parrotta, Maria Teresa De Angelis, Stefania Scalise, Patrizio Candeloro, Gianluca Santamaria, Mariagrazia Paonessa, Maria Laura Coluccio, Gerardo Perozziello, Stefania De Vitis, Antonella Sgura, Elisa Coluzzi, Vincenzo Mollace, Enzo Mario Di Fabrizio, Giovanni Cuda   +13 more
doaj   +1 more source

Histone deacetylase inhibitor valproic acid promotes the differentiation of human induced pluripotent stem cells into hepatocyte-like cells. [PDF]

PLoS ONE, 2014
In this study, we aimed to elucidate the effects and mechanism of action of valproic acid on hepatic differentiation from human induced pluripotent stem cell-derived hepatic progenitor cells.
Yuki Kondo, Takahiro Iwao, Sachimi Yoshihashi, Kayo Mimori, Ruri Ogihara, Kiyoshi Nagata, Kouichi Kurose, Masayoshi Saito, Takuro Niwa, Takayoshi Suzuki, Naoki Miyata, Shigeru Ohmori, Katsunori Nakamura, Tamihide Matsunaga   +13 more
doaj   +1 more source

Construction of 3D in vitro models by bioprinting human pluripotent stem cells: Challenges and opportunities [PDF]

, 2019
Three-dimensional (3D) printing of biological material, or 3D bioprinting, is a rapidly expanding field with interesting applications in tissue engineering and regenerative medicine. Bioprinters use cells and biocompatible materials as an ink (bioink) to
Rosa, Alessandro, Salaris, Federico
core   +1 more source

Human-Mouse Chimerism Validates Human Stem Cell Pluripotency [PDF]

Cell Stem Cell, 2016
Pluripotent stem cells are defined by their capacity to differentiate into all three tissue layers that comprise the body. Chimera formation, generated by stem cell transplantation to the embryo, is a stringent assessment of stem cell pluripotency.
Mascetti, Victoria L, Pedersen, Roger A
openaire   +3 more sources

Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin

Haematologica, 2015
Epigenetic memory in induced pluripotent stem cells, which is related to the somatic cell type of origin of the stem cells, might lead to variations in the differentiation capacities of the pluripotent stem cells.
Isabel Dorn, Katharina Klich, Marcos J. Arauzo-Bravo, Martina Radstaak, Simeon Santourlidis, Foued Ghanjati, Teja F. Radke, Olympia E. Psathaki, Gunnar Hargus, Jan Kramer, Martin Einhaus, Jeong Beom Kim, Gesine Kögler, Peter Wernet, Hans R. Schöler, Peter Schlenke, Holm Zaehres   +16 more
doaj   +1 more source