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Functional single-cell hybridoma screening using droplet-based microfluidics
Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they can be screened for binding affinities at very high throughput using systems such as phage ...
Debs, B. E. +4 more
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, 1999 Hybridoma cells utilize glutamine as their primary nitrogen source and excrete ammonia as a metabolic waste product. This ammonia can quickly accumulate to toxic levels in hybridoma culture media, and can severely reduce monoclonal antibody production ...
Okeson, Carl D.
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, 1987 Hybridoma cells which synthesize monoclonal antibodies (mAb) that block ricin toxicity were 50-300-fold resistant to ricin compared with other hybridomas.
COLOMBATTI, Marco, R. J. Youle
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Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells
COST-EFFECTIVE METHOD FOR FULL-LENGTH SEQUENCING OF MONOCLONAL ANTIBODIES FROM HYBRIDOMA CELLS Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells / Döring, Sarah (Rights reserved) (-
Flemig, Sabine +4 more
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Science, 1980
In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
openaire +2 more sources
An improved electrofusion technique for production of mouse hybridoma cells [PDF]
FEBS Letters, 1985 An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in
U. Zimmermann +3 more
exaly +2 more sources