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Cloning of Hybridomas

2003
Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
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A Mott cell hybridoma

European Journal of Immunology, 1984
AbstractA hybridoma is described that exhibits all the characteristic features of Mott cells. It has spherules (Russell bodies) in the cytoplasm made up of dilated rough endoplasmatic reticulum and containing condensed immunoglobulin (λ1 light chains). Some of the cells appear to be very fragile, and free spherules are often found on cell smears. Cells
Weiss, S.   +3 more
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NIH policies on hybridomas

In Vitro, 1981
partnership. Just as this new facility symbolizes the vigor of the ATCC, so this conference symbolizes the communal interest that must be accorded the preservation and distribution of microorganisms and other cell lines. The ATCC and other repositories are not simply means to facilitate the sharing of cells of contemporary interest.
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Central banking of hybridomas

In Vitro, 1981
The National Institute of Allergy and Infectious Diseases is developing, with the American Type Culture Collection, a central facility for the acquisition, maintenance, and distribution of hybridoma cell lines. This effort finds its principal justification in the great activity in this field and in the large variety of hybridoma lines that are produced
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Creating hybridomas by electrofusion

Nature, 1988
Electromanipulation has long been used to form pores in cell membranes to transfect them with foreign DNA. Now, the technique has been extended to the preparation of hybridomas.
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Process development for hybridoma cells

1988
Revue des facteurs a considerer pour l'optimisation de la production d'anticorps monoclonaux a partir de lignees d ...
Harbour, C   +2 more
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“Why Not Hybridomas”

Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2021
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Hybridoma technology: is it still useful?

Current Research in Immunology, 2021
Barbara Hamaguchi, Rodrigo Aguiar
exaly  

Hybridoma technology

La Ricerca in Clinica e in Laboratorio, 1984
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