Improved Protocol for Establishing CD4+ Hybridomas Specific for Human Class II MHC/Peptide Complex [PDF]
Bio-ProtocolAutoreactive CD4+ T cells are shaped by MHC class II–dependent selection, and HLA-DQ8 is a major susceptibility allele for type 1 diabetes and celiac disease.
Fatemehsadat Mousavinasab +4 more
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B cell receptor–based hybridoma enrichment enables discovery of antibodies against intracellular tumor antigens [PDF]
Frontiers in ImmunologyTumor-associated B cell responses are frequently observed in tumors and their draining lymph nodes, yet their antigen specificities and functional potential remain incompletely defined.
Iris Dotan +8 more
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Targeted fusion of antibody-secreting cells: Unlocking monoclonal antibody production with hybridoma technology [PDF]
mAbsHybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid the foundation for many of the current therapeutic, diagnostic, and research reagent ...
Fanny Rousseau +5 more
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Identification and validation of genes encoding humoral specificity for the chemical allergen toluene diisocyanate. [PDF]
PLoS ONEA panel of hybridomas specific for different isomers of toluene diisocyanate (TDI), a cross‑linking chemical used in polyurethane production, has been previously described.
Adam V Wisnewski, Jian Liu
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Virus-like Particles and Spectral Flow Cytometry for Identification of Dengue Virus-Specific B Cells in Mice and Humans [PDF]
VirusesSevere dengue virus (DENV) infections are associated with circulating non-neutralizing antibodies generated during heterotypic infections. Although antibodies are key mediators of both protection and pathogenesis, the specific dynamics of B cells (Bc ...
Katherine Segura +4 more
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Проблемы особо опасных инфекций, 2023 The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina +7 more
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Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice
Shiyan dongwu yu bijiao yixue, 2023 Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan +12 more
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Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. [PDF]
PLoS ONE, 2016 Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Yuki Kuniyoshi +9 more
doaj +1 more source
Проблемы особо опасных инфекций, 2021 Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina +6 more
doaj +1 more source
PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO
Ciência Animal Brasileira, 2014 The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in ...
Bruna Alves Devens +3 more
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