Results 1 to 10 of about 17,483 (156)

Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas [PDF]

PLoS ONE, 2016
Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding ...
Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi   +2 more
exaly   +2 more sources

Targeted fusion of antibody-secreting cells: Unlocking monoclonal antibody production with hybridoma technology [PDF]

mAbs
Hybridomas, the first method for creating monoclonal antibodies (mAbs), were reported 50 years ago. This approach, which transformed biomedical research and laid the foundation for many of the current therapeutic, diagnostic, and research reagent ...
Fanny Rousseau, Catherine Menier, Patricia Brochard, Stéphanie Simon, Karla Perez-Toralla, Anne Wijkhuisen   +5 more
doaj   +2 more sources

Identification and validation of genes encoding humoral specificity for the chemical allergen toluene diisocyanate. [PDF]

PLoS ONE
A panel of hybridomas specific for different isomers of toluene diisocyanate (TDI), a cross‑linking chemical used in polyurethane production, has been previously described.
Adam V Wisnewski, Jian Liu
doaj   +2 more sources

Virus-like Particles and Spectral Flow Cytometry for Identification of Dengue Virus-Specific B Cells in Mice and Humans [PDF]

Viruses
Severe dengue virus (DENV) infections are associated with circulating non-neutralizing antibodies generated during heterotypic infections. Although antibodies are key mediators of both protection and pathogenesis, the specific dynamics of B cells (Bc ...
Katherine Segura, Fabiola Martel, Manuel A. Franco, Federico Perdomo-Celis, Carlos F. Narváez   +4 more
doaj   +2 more sources

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2

Проблемы особо опасных инфекций, 2023
The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods.
G. V. Kuklina, S. S. Ipatov, A. S. Gorshkov, D. V. Pechenkin, A. V. Eremkin, A. V. Kuznetsovsky, A. S. Tumanov, I. V. Darmov   +7 more
doaj   +1 more source

Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice

Shiyan dongwu yu bijiao yixue, 2023
Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the ...
WANG Dan, ZHANG Xiaolu, WANG Yan, FU Bo, WANG Wendong, LIU Jing, ZHANG Suyin, WU Yihe, WU Deguo, DU Xiaoyan, ZHAN Dawei, ZHANG Xiulin, LI Changlong   +12 more
doaj   +1 more source

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types

Проблемы особо опасных инфекций, 2021
Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods.
G. V. Kuklina, S. S. Ipatov, D. V. Pechenkin, A. V. Eremkin, A. A. Kytmanov, M. Yu. Dubrovin, A. S. Gorshkov   +6 more
doaj   +1 more source

PRODUÇÃO DE HIBRIDOMAS SECRETORES DE ANTICORPOS ANTI- Neospora caninum PARA USO EM IMUNODIAGNÓSTICO

Ciência Animal Brasileira, 2014
The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in ...
Bruna Alves Devens, Marlene Isabel Vargas Viloria, Carlos Henryque de Souza e Silva, Joaquin Hernán Patarroyo Salcedo   +3 more
doaj   +1 more source

Obtaining Specific Hybridomas for Ki-67 Protein Immunodetection

Indonesian Biomedical Journal, 2021
BACKGROUND: Active proliferation is specific property of a tumor cells. However, the cost of the analysis is high due to commercial anti-Ki-67 mAbs used as the main immunoreagent for reliable identification of proliferating cells.
Aigerim Turgimbayeva, Sailau Abeldenov, Nurgul Sarina, Bekbolat Khassenov, Saule Eskendirova   +4 more
doaj   +1 more source

Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone

Veterinární Medicína, 2005
3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ).
M. Vass, L. Kotkova, I. Diblikova, Z. Nevorankova, K.M. Cooper, D.G. Kennedy, M. Franek   +6 more
doaj   +1 more source