Monoclonal antibodies against human astrocytomas and their reactivity pattern [PDF]
, 1987 The establishment of hybridomas after fusion of X63-Ag8.653 mouse myeloma cells and splenocytes from mice hyperimmunized against human astrocytomas is presented. The animals were primed with 5 × 106 chemically modified uncultured or cultured glioma cells.
B. Martin +36 more
core +1 more source
ER stress in antigen‐presenting cells promotes NKT cell activation through endogenous neutral lipids [PDF]
, 2020 CD1d-restricted invariant natural killer T (iNKT) cells constitute a common glycolipid-reactive innate-like T-cell subset with a broad impact on innate and adaptive immunity.
Cheng, Tan‐Yun +12 more
core +2 more sources
MANUFACTURING OF HYBRIDOMAS-PRODUCERS OF MONOCLONAL ANTIBODIES TO BRUCELLOSIS AGENT ANTIGENS
Проблемы особо опасных инфекций, 2017 Objective of study is to prepare hybridomas-producers of monoclonal antibodies to brucellosis agent antigens. Materials and methods. B. abortus, B. melitensis, B.
G. V. Kuklina +5 more
doaj +1 more source
New simplified molecular design for functional T cell receptor [PDF]
, 1993 We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain.
Clevers +23 more
core +1 more source
Correlation between cell aggregation and antibody production in IgE-producing plasma cells
Biochemistry and Biophysics Reports, 2017 Allergic conditions result in the increase of immunoglobulin (Ig)E-producing plasma cells (IgE-PCs); however, it is unclear how IgE production is qualitatively controlled.
Mari Hikosaka +3 more
doaj +1 more source
Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites [PDF]
, 2016 Autoantibodies to insulin are a harbinger of autoimmunity in type 1 diabetes in humans and in non-obese diabetic mice. To understand the genesis of these autoantibodies, we investigated the interactions of insulin-specific T and B lymphocytes using T ...
Acevedo-Suárez +59 more
core +2 more sources
Is cancer progression caused by gradual or simultaneous acquisitions of new chromosomes?
Molecular Cytogenetics, 2018 Background Foulds defined, “Tumor progression (as a) permanent, irreversible qualitative change in one or more of its characters” (Cancer Res. 1954).
Mathew Bloomfield, Peter Duesberg
doaj +1 more source
Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
Nature Communications, 2016 Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
Mark Pogson +4 more
doaj +1 more source
A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.
PLoS ONE, 2021 The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives.
Ghasidit Pornnoppadol +6 more
doaj +1 more source
A New Workflow to Generate Monoclonal Antibodies against Microorganisms
Applied Sciences, 2021 Monoclonal antibodies are used worldwide as highly potent and efficient detection reagents for research and diagnostic applications. Nevertheless, the specific targeting of complex antigens such as whole microorganisms remains a challenge.
Markus Göthel +5 more
doaj +1 more source