Results 21 to 30 of about 19,999 (251)

When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions [PDF]

, 2018
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
Shelley Force Aldred, Jacob Glanville, Glanville, Jacob, Eng, Hui-Yan, Tandon, Atul, Stefan Dübel, Ferrara, Fortunato, Stephen Anderson, Frenzel, Andre, Wong, Julin, Trimmer, James, Görnemann, Janina, Dübel, Stefan, Trimmer, James S, Koh, Xin, Aldred, Shelley, Atul K. Tandon, Aldred, Shelley Force, Wilkinson, Ian, Jennifer Maynard, Anderson, Stephen, Bestagno, Marco, Clark, Mike, Xin Yu Koh, Wilkinson, Ian C, Burrone, Oscar, Mike Clark, Tandon, Atul K, Maynard, Jennifer, Marie-Paule Lefranc, Trinklein, Nathan D, Catherine L. Bladen, Julin Wong, Hui-Yan Eng, Lane, David, Bladen, Catherine L, Holger Thie, Thie, Holger, James S. Trimmer, Philipp Wolf, Bradbury, Andrew, Koh, Xin Yu, Andrew R. M. Bradbury, Trinklein, Nathan, Oscar Burrone, Lefranc, Marie-Paule, Marco Bestagno, Bladen, Catherine, Jones, Brittany, Brittany Jones, Ian C. Wilkinson, David Lane, Fortunato Ferrara, Bradbury, Andrew RM, Janina Görnemann, Wolf, Philipp, Nathan D. Trinklein, Andre Frenzel   +57 more
core   +1 more source

Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

Nature Communications, 2016
Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
Mark Pogson, Cristina Parola, William J. Kelton, Paul Heuberger, Sai T. Reddy   +4 more
doaj   +1 more source

Preparation and Biological Activity of the Monoclonal Antibody against the Second Extracellular Loop of the Angiotensin II Type 1 Receptor

Journal of Immunology Research, 2016
The current study was to prepare a mouse-derived antibody against the angiotensin II type 1 receptor (AT1-mAb) based on monoclonal antibody technology, to provide a foundation for research on AT1-AA-positive diseases.
Mingming Wei, Chengrui Zhao, Suli Zhang, Li Wang, Huirong Liu, Xinliang Ma   +5 more
doaj   +1 more source

A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.

PLoS ONE, 2021
The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives.
Ghasidit Pornnoppadol, Boya Zhang, Alec A Desai, Anthony Berardi, Henriette A Remmer, Peter M Tessier, Colin F Greineder   +6 more
doaj   +1 more source

A New Workflow to Generate Monoclonal Antibodies against Microorganisms

Applied Sciences, 2021
Monoclonal antibodies are used worldwide as highly potent and efficient detection reagents for research and diagnostic applications. Nevertheless, the specific targeting of complex antigens such as whole microorganisms remains a challenge.
Markus Göthel, Martin Listek, Katrin Messerschmidt, Anja Schlör, Anja Hönow, Katja Hanack   +5 more
doaj   +1 more source

Utilization of Immunoblotting in Studies of Epitope Targeting in Monoclonal Antibodies to Melioidosis Agent Antigen 200 kDa

Проблемы особо опасных инфекций, 2014
Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova, N. P. Khrapova, T. V. Zamarina, E. V. Pimenova, N. S. Makarov, Yu. A. Goloseev   +5 more
doaj   +1 more source

Hybridoma technology; advancements, clinical significance, and future aspects

Journal of Genetic Engineering and Biotechnology, 2021
Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal ...
Sanchita Mitra, Pushpa Chaudhary Tomar
doaj   +1 more source

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Iranian Journal of Parasitology, 2022
Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases ...
Asli Pinar Zorba Yildiz, Gulnaz Yildirim Koken, Emrah Sefik Abamor, Melahat Bagirova, Ozlem Ayse Tosyali, Tanil Kocagoz, Adil Allahverdiyev   +6 more
doaj   +1 more source

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

BioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost   +5 more
doaj   +1 more source

Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses

Viruses, 2023
Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng, Jin-Ling Liu, Qin Luo, Lu-Ping Zheng, Yongxiu Yao, Venugopal Nair, Gai-Ping Zhang, Jun Luo   +7 more
doaj   +1 more source