MANUFACTURING OF HYBRIDOMAS-PRODUCERS OF MONOCLONAL ANTIBODIES TO BRUCELLOSIS AGENT ANTIGENS [PDF]
Проблемы особо опасных инфекций, 2017 Objective of study is to prepare hybridomas-producers of monoclonal antibodies to brucellosis agent antigens. Materials and methods. B. abortus, B. melitensis, B.
G. V. Kuklina +5 more
doaj +2 more sources
Cryopreservation of Hybridomas
, 2003 Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
Gustafsson, B
openaire +3 more sources
, 2003 Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
Gustafsson, B
openaire +3 more sources
Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens [PDF]
Проблемы особо опасных инфекций, 2015 Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov +9 more
doaj +2 more sources
Development and characterization of novel chimeric monoclonal antibodies for broad spectrum neutralization of rabies virus. [PDF]
PLoS ONE, 2017 Current post-exposure prophylaxis for rabies virus infection has several limitations in terms of supply, cost, safety, and efficacy. Attempts to replace human or equine rabies immune globulins (HRIG or ERIG) have been made by several companies and ...
Pan Kyeom Kim +18 more
doaj +1 more source
Frontiers in Immunology, 2023 IntroductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), had a major impact on both the global health and economy.
Chang-Kyu Heo +9 more
doaj +1 more source
Trinitrophenol Reactive T-Cell Hybridomas Recognize Antigens That Require Antigen Processing [PDF]
, 1994 Protein antigens must be taken up, processed, and displayed on the surface of antigen-presenting cells in association with major histocompatibility complex molecules before they can be recognized by T cells.
Sy, Man-Sun +5 more
core +1 more source
A comparison of three methods for production of human hybridomas secreting autoantibodies
, 1998 Human hybridomas that secrete monoclonal antibodies (MAbs) in a stable manner are technically difficult to obtain. The problems limiting their production are the low numbers of sensitized B cells in peripheral blood, the limited choice of techniques for ...
Niedbala, W.G., Stott, D.I.
core +8 more sources
Correlation between cell aggregation and antibody production in IgE-producing plasma cells
Biochemistry and Biophysics Reports, 2017 Allergic conditions result in the increase of immunoglobulin (Ig)E-producing plasma cells (IgE-PCs); however, it is unclear how IgE production is qualitatively controlled.
Mari Hikosaka +3 more
doaj +1 more source
When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions [PDF]
, 2018 Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
Shelley Force Aldred +57 more
core +1 more source