Results 31 to 40 of about 34,653 (293)

Utilization of Immunoblotting in Studies of Epitope Targeting in Monoclonal Antibodies to Melioidosis Agent Antigen 200 kDa

Проблемы особо опасных инфекций, 2014
Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova, N. P. Khrapova, T. V. Zamarina, E. V. Pimenova, N. S. Makarov, Yu. A. Goloseev   +5 more
doaj   +1 more source

Hybridoma technology; advancements, clinical significance, and future aspects

Journal of Genetic Engineering and Biotechnology, 2021
Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal ...
Sanchita Mitra, Pushpa Chaudhary Tomar
doaj   +1 more source

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Iranian Journal of Parasitology, 2022
Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases ...
Asli Pinar Zorba Yildiz, Gulnaz Yildirim Koken, Emrah Sefik Abamor, Melahat Bagirova, Ozlem Ayse Tosyali, Tanil Kocagoz, Adil Allahverdiyev   +6 more
doaj   +1 more source

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

BioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost   +5 more
doaj   +1 more source

Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses

Viruses, 2023
Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng, Jin-Ling Liu, Qin Luo, Lu-Ping Zheng, Yongxiu Yao, Venugopal Nair, Gai-Ping Zhang, Jun Luo   +7 more
doaj   +1 more source

Function of B cells expressing a human immunoglobulin M rheumatoid factor autoantibody in transgenic mice. [PDF]

, 1993
We have generated transgenic mice that express the immunoglobulin (Ig)M heavy chain and kappa light chain genes coding for a human IgM rheumatoid factor (RF), Les.
Carson, DA, Chen, PP, Jirik, FR, Kipps, TJ, Roudier, J, Tighe, H, Tucker, R   +6 more
core   +2 more sources

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

Проблемы особо опасных инфекций, 2015
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov, G. D. Elagin, G. V. Kuklina, D. V. Pechenkin, V. V. Krupin, N. V. Bogacheva, A. A. Kytmanov, A. V. Eremkin, O. V. Tikhvinskaya, E. Yu. Vakhnov   +9 more
doaj   +1 more source

Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies

Open Veterinary Journal, 2023
Background: African Swine Fever (ASF) is an infectious disease that affects domestic pig and wild boar populations. The African Swine Fever Virus (ASFV) has a genome characterized by a very complex DNA (170-193 kb) that encodes for more than 200 ...
Rosrio Liberti, Claudia Colabella, Lucia Anzalone, Giulio Severi, Antonella Di Paolo, Cristina Casciari, Anna Beatrice Casano, Monica Giammarioli, Monica Cagiola, Francesco Feliziani, Antonio De Giuseppe   +10 more
doaj   +1 more source

Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors

Cell Reports, 2018
Summary: The pathogenic potential of Mycobacterium tuberculosis largely depends on ESX secretion systems exporting members of the multigenic Esx, Esp, and PE/PPE protein families.
Fadel Sayes, Catherine Blanc, Louis S. Ates, Nathalie Deboosere, Mickael Orgeur, Fabien Le Chevalier, Matthias I. Gröschel, Wafa Frigui, Ok-Ryul Song, Richard Lo-Man, Florence Brossier, Wladimir Sougakoff, Daria Bottai, Priscille Brodin, Pierre Charneau, Roland Brosch, Laleh Majlessi   +16 more
doaj   +1 more source

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells

Artificial Cells, Nanomedicine, and Biotechnology
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the ...
Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh   +9 more
doaj   +1 more source