Results 31 to 40 of about 3,737 (192)

Characterization of Autoreactive CD4+ T cell Hybridomas [PDF]

Autoimmune diseases are diverse disorders arising from abnormal cellular and humoral responses to self-antigens in the immune system. However, the underlying cause of autoimmunity remains unclear.
Raji-Ayoola, Jumoke Ayodele
core   +1 more source

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells

Artificial Cells, Nanomedicine, and Biotechnology
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the ...
Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh   +9 more
doaj   +1 more source

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

BioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost   +5 more
doaj   +1 more source

Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses

Viruses, 2023
Marek’s disease (MD) caused by pathogenic Marek’s disease virus type 1 (MDV−1) is one of the most important neoplastic diseases of poultry. MDV−1-encoded unique Meq protein is the major oncoprotein and the availability of Meq-specific monoclonal ...
Man Teng, Jin-Ling Liu, Qin Luo, Lu-Ping Zheng, Yongxiu Yao, Venugopal Nair, Gai-Ping Zhang, Jun Luo   +7 more
doaj   +1 more source

Epitope mapping analyses of hybridomas generated.

, 2015
Hybridomas were evaluated for reactivity against gp120, gp120-OD, BG505 SOSIP gp140, as well as eOD-GT6 and a large panel of peptides. Hybridomas are arranged in groups based on their linear epitopes or their reactivity to three proteins. NT: Not Tested.
Michael W. Cho (210791), David C. Montefiori (108747), Aditi Agrawal (749234), Marisa Banasik (749236), Kari Rohl (749237), Yali Qin (749233), Saikat Banerjee (473461), Feng Lin (23861), Celia LaBranche (170898), Heliang Shi (749235)   +9 more
core   +1 more source

Improved Protocol for Establishing CD4+ Hybridomas Specific for Human Class II MHC/Peptide Complex

Bio-Protocol
Autoreactive CD4+ T cells are shaped by MHC class II–dependent selection, and HLA-DQ8 is a major susceptibility allele for type 1 diabetes and celiac disease.
Fatemehsadat Mousavinasab, Edyta Szurek, Anna Cebula, Leszek Ignatowicz, Michal Kuczma   +4 more
doaj   +1 more source

Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies

Open Veterinary Journal, 2023
Background: African Swine Fever (ASF) is an infectious disease that affects domestic pig and wild boar populations. The African Swine Fever Virus (ASFV) has a genome characterized by a very complex DNA (170-193 kb) that encodes for more than 200 ...
Rosrio Liberti, Claudia Colabella, Lucia Anzalone, Giulio Severi, Antonella Di Paolo, Cristina Casciari, Anna Beatrice Casano, Monica Giammarioli, Monica Cagiola, Francesco Feliziani, Antonio De Giuseppe   +10 more
doaj   +1 more source

Sponge Hybridomas: Applications and Implications

, 2013
Many sponge-derived natural products with applications to human health have been discovered over the past three decades. In vitro production has been proposed as one biological alternative to ensure adequate supply of marine natural products for ...
Pomponi, S.A., Jevitt, A., Patel, J., Diaz, M.C.   +3 more
core   +1 more source

Optical Detection of Cellular Signals at Material Interfaces

Advanced Healthcare Materials, EarlyView.
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren, Jitong Ren, Zixin Zhang, Koney Eungkon Kim, Francis L. Hong, Yuecheng Peter Zhou   +5 more
wiley   +1 more source

Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors

Cell Reports, 2018
Summary: The pathogenic potential of Mycobacterium tuberculosis largely depends on ESX secretion systems exporting members of the multigenic Esx, Esp, and PE/PPE protein families.
Fadel Sayes, Catherine Blanc, Louis S. Ates, Nathalie Deboosere, Mickael Orgeur, Fabien Le Chevalier, Matthias I. Gröschel, Wafa Frigui, Ok-Ryul Song, Richard Lo-Man, Florence Brossier, Wladimir Sougakoff, Daria Bottai, Priscille Brodin, Pierre Charneau, Roland Brosch, Laleh Majlessi   +16 more
doaj   +1 more source