Results 51 to 60 of about 23,113 (239)

Comparative Analysis of Bioreactor Design and Scale‐Up for Cultivated Meat Using Monte Carlo‐Based Timescale Modeling

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cultivated meat offers a sustainable alternative to conventional livestock production but faces significant scale‐up challenges, particularly in bioreactor design for mammalian cell suspension cultures. This study presents a comparative analysis of stirred tank reactors (STR), bubble columns (BC), and airlift reactors (ALR) to assess their ...
Pieter Jan Theodoor Brorens, Marcel Ottens, Cees Haringa   +2 more
wiley   +1 more source

Immunoglobulin heavy chain variable region gene utilization by B cell hybridomas derived from rheumatoid synovial tissue

, 1992
SUMMARY Rheumatoid arthritis (RA) is a chronic inflammatory disease that primarily affects synovial joints. Activated B lymphocytes and plasma cells are present in the synovial tissue and are thought to contribute to the immunopathology of
G HAYNES, C M S BROWN, A MALCOLM, C PLATER-ZYBERK, C LONGHURST, R N MAINI   +5 more
core   +1 more source

Retinoic acid induces the differentiation of B cell hybridomas from patients with common variable immunodeficiency. [PDF]

, 1988
Human-human B cell hybridomas constructed from B lymphocytes of common variable immunodeficiency (CVI) patients and the nonsecreting cell line WIL2/729 HF consistently secrete low levels of Ig and appear to retain a defect characteristic of the CVI ...
D C Adelman, M Gilly, R Wall, N Sidell, Adelman, DC, Wall, R, Gilly, M, Sidell, N, A Saxon, Saxon, A, E Sherr, Sherr, E   +11 more
core   +1 more source

A review of the inhibitory waste metabolites produced during mAb biomanufacturing

Biotechnology Progress, EarlyView.
The review highlights inhibitory metabolites in CHO cell cultures, such as lactate, ammonia, isobutyrate, and isovalerate, impacting growth and productivity. Identification methods include LC/MS‐based metabolomics, while control strategies involve media optimization, genetic engineering, and process adjustments (pH, temperature).
Zhao Wang, Caitlin Morris, Duc Hoang, Jianlin Xu, Michael C. Borys, Seongkyu Yoon   +5 more
wiley   +1 more source

Monoclonal Antibodies; Detection Tools for Wheat Alpha‐Amylase Proteins

Cereal Chemistry, EarlyView.
ABSTRACT Background and Objectives Expression of alpha‐amylase proteins during germination or other physiological events may lower grain soundness and have a negative impact on end‐use quality. The objective of the current study was to validate two new monoclonal peptide antibodies for wheat alpha‐amylases and describe alpha‐amylase expression in ...
John H. Kelly, Dru Mattei, Andrew G. McCubbin, Camille M. Steber, Michael O. Pumphrey, Amber L. Hauvermale   +5 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers

Antibodies, 2020
Monoclonal antibodies (mAbs) that recognize glycans are useful tools to assess carbohydrates’ structure and function. We sought to produce IgG mAbs to the human milk oligosaccharide (HMO), lacto-N-fucopentaose III (LNFPIII).
Jessica Ramadhin, Vanessa Silva-Moraes, Thomas Norberg, Donald Harn   +3 more
doaj   +1 more source

Increased Insulin Action, Glucose Metabolism and Muscle Function in Supervillin‐Knockout and Supervillin‐Mutant Mice

Cytoskeleton, EarlyView.
ABSTRACT We here describe mouse models with complementary homozygous Svil mutations. In skeletal muscle, Svil‐Mut mice express the Svil‐encoded N‐terminus fused to the βgal‐neo gene‐trap tag and lack the highly conserved archvillin C‐terminus; Svil‐KO mice lack expression of all known Svil‐encoded proteins; and Svil‐LoxP mice contain loxP sites for ...
Tara C. Smith, Lauren A. Tauer, Randall H. Friedline, Xiaodi Hu, Duy Tran, Yangyi E. Luo, Nacima Hadjout‐Rabi, Elisabeth R. Barton, Jason K. Kim, Elizabeth J. Luna   +9 more
wiley   +1 more source

Ex vivo generation of human anti-melanoma autologous cytolytic T cells by dendritic cell/melanoma cell hybridomas

, 2001
Due to their central role in controlling immunity, dendritic cells are logical targets for priming naive cytotoxic T lymphocytes against tumour cells.
Zwirner, Joerg, Neumann, C., Matthes, C., Riggert, Joachim, Soruri, Afsaneh, Fayyazi, Afshin, Peters, J. Hinrich, Schlott, T., Jung, Tobias   +8 more
core   +1 more source

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.

PLoS ONE, 2015
B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology.
Haolin Liu, Janice White, Frances Crawford, Niyun Jin, Xiangwu Ju, Kangtai Liu, Chengyu Jiang, Philippa Marrack, Gongyi Zhang, John W Kappler   +9 more
doaj   +1 more source