Results 61 to 70 of about 3,737 (192)

Three‐dimensional observation of the muscle–tendon integration process in mouse embryos

Developmental Dynamics, EarlyView.
Abstract Background Muscle–tendon integration is a crucial step in the morphogenesis of the vertebrate locomotion system. As the limb muscle and tendon progenitor cells derive from distinct embryonic origin, their integration requires precise mutual positioning.
Ramu Sagasaki, Anzu Taniguchi, Shunsuke Oka, Mizuki Mishima, Yuki Yamasaki, Yutaro Kawa, Saundra Schlesinger, Ryo Fujita, Chisa Shukunami, Hiroshi Asahara, Masafumi Inui   +10 more
wiley   +1 more source

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas.

PLoS ONE, 2015
B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology.
Haolin Liu, Janice White, Frances Crawford, Niyun Jin, Xiangwu Ju, Kangtai Liu, Chengyu Jiang, Philippa Marrack, Gongyi Zhang, John W Kappler   +9 more
doaj   +1 more source

Development of a new monoclonal antibody specific to mouse Vγ6 chain

Life Science Alliance, 2019
Using a novel mAb specific to Vγ6 TCR, we find Vγ6 + γδT cells are located in association with medullary thymic epithelial cells and play crucial roles in protection against Klebsiella pneumoniae infection but are pathogenic in psoriasis-like dermatitis ...
Shinya Hatano, Xin Tun, Naoto Noguchi, Dan Yue, Hisakata Yamada, Xun Sun, Mitsuru Matsumoto, Yasunobu Yoshikai   +7 more
doaj   +1 more source

Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers

Antibodies, 2020
Monoclonal antibodies (mAbs) that recognize glycans are useful tools to assess carbohydrates’ structure and function. We sought to produce IgG mAbs to the human milk oligosaccharide (HMO), lacto-N-fucopentaose III (LNFPIII).
Jessica Ramadhin, Vanessa Silva-Moraes, Thomas Norberg, Donald Harn   +3 more
doaj   +1 more source

DUCK‐Net: automated deep learning segmentation of ductular reactions in murine liver injury captures multicellular niche dynamics from H&E morphology

The Journal of Pathology, EarlyView.
Abstract Ductular reactions (DRs) are dynamic and complex multicellular responses that occur as a result of various hepatic injuries. Precise identification and quantification of the extent of DRs is a cornerstone of pre‐clinical modelling of liver disease, with links to inflammation, fibrosis, regeneration, and disease severity.
Nathalie Feeley, Kai Williams, Caitlin McCaffrey, Daniel Field, Kyle Davies, Hugh Warden, Luke Boulter, Steve Thorn, Tak Yung Man, Wei‐Yu Lu, Stephen J Wigmore, Ewen M Harrison, Stuart J Forbes, Ian P Tomlinson, Timothy J Kendall, Rachel V Guest   +15 more
wiley   +1 more source

Electrochemical and Biological Evaluation of Patterned TiO2@TiC‐Nanowires for Bio‐Interface Electrode

Small, EarlyView.
This study introduces a room‐temperature process to fabricate patterned TiO2@TiC core–shell nanowires, merging the biocompatibility of TiO2‐NWs with the high conductivity of TiC. Through sputtering pressure and time modulation, the nanowires morphology and electrochemical properties are tuned, enabling enhanced capacitance, stability, and guided ...
Roaa Sait, Shofarul Wustoni, Nabeel Aslam, Camelia Florica, Andrea Diaz‐Gaxiola, Valentina‐Elena Musteata, Mohammed K. Drad, Stephen Ghandour, Georgian Melinte, Yaping Zhang, Mohammed Nejib Hedhili, Ahad Ali Syed, Menouer Saidani, Shahryar Khattak, Imed Eddine‐Gallouzi, Sahika Inal, Hala Aljawhari   +16 more
wiley   +1 more source

Immunochemical evidence that human apoB differs when expressed in rodent versus human cells

Journal of Lipid Research, 2003
LDL from human apolipoprotein B-100 (apoB-100) transgenic (HuBTg+/+) mice contains more triglyceride than LDL from normolipidemic subjects. To obtain novel monoclonal antibody (MAb) probes of apoB conformation, we generated hybridomas from HuBTg+/+ that ...
Xingyu Wang, Vinita Chauhan, Anh T. Nguyen, Joshua Schultz, Jean Davignon, Stephen G. Young, Jan Borén, Thomas L. Innerarity, Hui Rutai, Ross W. Milne   +9 more
doaj   +1 more source

Safety of and serological response to a new canine distemper‐parvovirus combination vaccine in domestic ferrets

Veterinary Record, EarlyView.
Abstract Background Pet ferrets are typically vaccinated against distemper using a canine multivalent vaccine despite the lack of relevant safety or efficacy data. Here, we investigate the safety of a novel live canine parvovirus type 2c (CPV‐2c) vaccine strain and the efficacy of the live Onderstepoort canine distemper virus (CDV) vaccine strain used ...
Jacqueline Pearce, Léon Peeters, Ellen Versmissen, David Sutton, Ian Tarpey   +4 more
wiley   +1 more source

Diagnostic antibodies at an inflection point: From discovery platforms to design‐oriented engineering

VIEW, EarlyView.
This study systematically organizes the evolution of diagnostic antibodies from discovery platforms to design‐oriented engineering and analyzes the strengths and limitations of each approach. Meanwhile, it provides a critical, literature‐driven synthesis of current challenges, proposing integrated strategies for assay‐aware and function‐driven antibody
Qitao Song, Lulai Xu, Weilin Wu, Fei Xie, Yantong Pan, Tiantian Yang, Xiaoxia Xu, Yanyin Lin, Lihua Zhang, Jiahui Zhou, Junyou Zhou, Linping Li, Zhanhui Wang, Weijing Yi, Jing Wang   +14 more
wiley   +1 more source

Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression

Antibodies, 2020
Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains.
Rama Devudu Puligedda, Vladimir Vigdorovich, Diana Kouiavskaia, Chandana Devi Kattala, Jiang-yang Zhao, Fetweh H. Al-Saleem, Konstantin Chumakov, D. Noah Sather, Scott K. Dessain   +8 more
doaj   +1 more source