Results 61 to 70 of about 19,999 (251)
Cytoskeleton, EarlyView.ABSTRACT Elastic tethers connect telomeres of separating chromosomes in anaphase of animal cells. Immunofluorescence staining of titin in crane‐fly spermatocytes, using 4 different antibodies, shows that the giant elastic protein titin seems to be a component of mitotic tethers: titin “strands” extend between separating chromosomes, connecting their ...
Demetra Economopoulos +5 more
wiley +1 more source
Immunochemical evidence that human apoB differs when expressed in rodent versus human cells
Journal of Lipid Research, 2003 LDL from human apolipoprotein B-100 (apoB-100) transgenic (HuBTg+/+) mice contains more triglyceride than LDL from normolipidemic subjects. To obtain novel monoclonal antibody (MAb) probes of apoB conformation, we generated hybridomas from HuBTg+/+ that ...
Xingyu Wang +9 more
doaj +1 more source
, 2022 Hybridoma technology is widely used for monoclonal antibody (mAb) discovery, whereas the generation and identification of single hybridomas by the limiting dilution method (LDM) are tedious, inefficient, and time- and cost-consuming, especially for ...
Yuebin Ke +15 more
core +1 more source
Developmental Dynamics, EarlyView.Abstract Background Robinow syndrome is a rare developmental syndrome caused by variants in genes in Wnt signaling pathways. We previously showed that expression of patient variants in Dishevelled 1 (DVL1) in Drosophila and chicken models disrupts the balance of canonical and non‐canonical Wnt signaling.
Gamze Akarsu +4 more
wiley +1 more source
Fhod3 in zebrafish supports myofibril stability during growth of embryonic skeletal muscle
Developmental Dynamics, EarlyView.Abstract Background Actin filament organization in cardiomyocytes critically depends on the formin Fhod3, but a role for Fhod3 in skeletal muscle development has not yet been described. Results We demonstrate here that in zebrafish mutated for one of two fhod3 paralog genes, fhod3a, skeletal muscle of the trunk appears normal through 2 days post ...
Aubrie Russell +3 more
wiley +1 more source
Antibodies, 2020 Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains.
Rama Devudu Puligedda +8 more
doaj +1 more source
Journal of Immunology Research, 2021 Tetranor-PGDM is a metabolite of PGD2. Urinary tetranor-PGDM levels were reported to be increased in some diseases, including food allergy, Duchenne muscular dystrophy, and aspirin-intolerant asthma. In this study, we developed a monoclonal antibody (MAb)
Nanae Nagata +5 more
doaj +1 more source
, 2001 Due to their central role in controlling immunity, dendritic cells are logical targets for priming naive cytotoxic T lymphocytes against tumour cells.
Zwirner, Joerg +8 more
core +1 more source
Food Safety and Health, EarlyView.A sensitive colloidal gold immunochromatographic assay for atrazine was successfully established using genetically engineered nanobody‐recombinant antibody, which overcame the shortcomings of traditional antigen‐antibody. ABSTRACT Colloidal gold immunochromatography (CG‐ICA) is distinguished by its cost‐effectiveness and rapid detection capabilities ...
Jiayong Bao +16 more
wiley +1 more source
Potent monoclonal antibodies against multidrug‐resistant hypervirulent Klebsiella pneumoniae
Interdisciplinary Medicine, EarlyView.A novel immunization strategy using a low‐virulence, multidrug‐resistant strain yields synergistic monoclonal antibodies against hypervirulent Klebsiella pneumoniae. These antibodies provide cross‐serotype protection through a dual‐mechanism of pathogen clearance and immunomodulation, offering a promising non‐antibiotic therapeutic for resistant ...
Yushan Jiang +10 more
wiley +1 more source