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Cryopreservation of Hybridomas
, 2003 Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
Gustafsson, B
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, 2003 Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
Gustafsson, B
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When Monoclonal Antibodies Are Not Monospecific: Hybridomas Frequently Express Additional Functional Variable Regions [PDF]
MAbs, 2018 Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas.
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Science, 1980
In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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Cold Spring Harbor Protocols, 2021
This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
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This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
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Cell hybridization, hybridomas, and human hybridomas.
Methods in cell biology, 1998Cell hybridization is one of the most basic cytotechnologies. The hemagglutinating virus of Japan was first used to cause cell fusion; however, polyethylene glycol is widely used now because of simplicity of procedure. This chapter first explains the principles of cell hybridization methods and then describes the practical protocols for preparing mouse
S, Shirahata, Y, Katakura, K, Teruya
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2005
Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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2015
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
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The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
openaire +2 more sources