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PD-1 suppresses CAR signaling by forming the inhibitory signalosome colocalizing to CAR microclusters. [PDF]
Commun BiolYoshida Y +10 more
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Cryopreservation of Hybridomas
, 2003 Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
Gustafsson, B
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, 2003 Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
Gustafsson, B
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Cryopreservation of newly formed hybridomas
Journal of Immunological Methods, 1983A new freezing technique is described which permits time-consuming protocols as a first screening of newly formed hybridomas. In this procedure complete 96 well clustertrays with growing hybridomas are cryopreserved after programmed freezing. This procedure has been successfully applied to a number of fusion protocols for which the objective was to ...
L De Leij, T H The, T H The
exaly +4 more sources
Science, 1980
In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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European Journal of Immunology, 1994 Udgivelsesdato: 1994-JunA panel of antigen-specific, major histocompatibility complex class I-restricted T cell hybridomas has been generated to examine the capacity of peptide/class I complexes to stimulate T cells at the molecular level.
Anette Stryhn +2 more
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Cold Spring Harbor Protocols, 2021
This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
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This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
openaire +2 more sources