Results 61 to 70 of about 59,454 (330)

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.

Proceedings of the National Academy of Sciences of the United States of America, 1980
We report the establishment of human-human hybridomas producing monoclonal antibody of predefined antigenic specificity. The U-266 human myeloma cell line was incubated in the presence of 8-azaguanine, and a rapidly growing, 8-azaguanine-resistant ...
L. Olsson, H. Kaplan
semanticscholar   +1 more source

Polymer Nanogels with Albumin Reporting Interface Created Using RAFT Precipitation Polymerization for Disease Diagnosis and Food Testing

Advanced Materials Interfaces, EarlyView.
Herein, polymer nanogels with a reporting interface for albumin are synthesized through reversible addition–fragmentation chain transfer (RAFT) precipitation polymerization of di(ethylene glycol) methyl ether methacrylate and N,N’‐methylenebis(acrylamide) using a poly(2‐methacryloyloxyethyl phosphorylcholine) macro‐RAFT agent, followed by dansyl ...
Yukiya Kitayama, Erika Yoshimatsu, Atsushi Harada   +2 more
wiley   +1 more source

The non-palindromic adaptor-PCR method for the identification of the T-cell receptor genes of an interferon-gamma-secreting T-cell hybridomaspecific for trans-sialidase, an immunodominant Trypanosoma cruzi antigen

Brazilian Journal of Medical and Biological Research, 2006
Cloning of the T-cell receptor genes is a critical step when generating T-cell receptor transgenic mice. Because T-cell receptor molecules are clonotypical, isolation of their genes requires reverse transcriptase-assisted PCR using primers specific for ...
M.I. Hiyane, S.B. Boscardin, M.M. Rodrigues   +2 more
doaj   +1 more source

Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression

Antibodies, 2020
Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains.
Rama Devudu Puligedda, Vladimir Vigdorovich, Diana Kouiavskaia, Chandana Devi Kattala, Jiang-yang Zhao, Fetweh H. Al-Saleem, Konstantin Chumakov, D. Noah Sather, Scott K. Dessain   +8 more
doaj   +1 more source

Antigen presentation by hapten-specific B lymphocytes. I. Role of surface immunoglobulin receptors. [PDF]

, 1984
The present study examines the ability of hapten-specific murine splenic B lymphocytes to present hapten-proteins to carrier-specific T cell hybridomas.
Abbas, AK, Benacerraf, B, Rock, KL
core  

Absence of Functional Autoantibodies Targeting Angiotensin II Receptor Type 1 and Endothelin‐1 Type A Receptor in Circulation and Purified IgG From Patients With Systemic Sclerosis

Arthritis &Rheumatology, EarlyView.
Objective Systemic sclerosis (SSc) is a rare but severe autoimmune disease characterized by immune dysregulation, fibrosis, and vasculopathy. Although previous studies have highlighted the presence of functional autoantibodies targeting the angiotensin II receptor type 1 (AT1) and endothelin‐1 type A receptor (ETAR), leading to autoantibody‐mediated ...
Wieke M. van Oostveen, Eva M. Hoekstra, E. W. Nivine Levarht, Ilana B. Kotliar, Thomas P. Sakmar, René E. M. Toes, Jeska K. de Vries‐Bouwstra, Laura H. Heitman, Cynthia M. Fehres   +8 more
wiley   +1 more source

Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers

Antibodies, 2020
Monoclonal antibodies (mAbs) that recognize glycans are useful tools to assess carbohydrates’ structure and function. We sought to produce IgG mAbs to the human milk oligosaccharide (HMO), lacto-N-fucopentaose III (LNFPIII).
Jessica Ramadhin, Vanessa Silva-Moraes, Thomas Norberg, Donald Harn   +3 more
doaj   +1 more source

Application of In-Cell ELISA assay for hybridoma screening and selection of promising producers of monoclonal antibodies

Учёные записки Казанского университета: Серия Естественные науки, 2022
This study was motivated by the pressing need to obtain monoclonal antibodies (mAb) for research and medical purposes. Screening for mAb-producing hybridomas and evaluating various cell cultures requires a technique that combines the advantages of ...
I.I. Stepanova, K.A. Artemyeva, A.A. Stepanov, I.M. Bogdanova, E.A. Ponomarenko, M.N. Boltovskaya   +5 more
doaj   +1 more source

Monoclonal Antibodies to Thyrotropin Receptor With Thyroid-Stimulating Activity Activate the NF-κB Pathway to Induce Chemokine Expression. [PDF]

J Cell Mol Med
ABSTRACT The A subunit of thyrotropin receptor (TSHR) is thought to be the crucial gene mediating stimulatory autoantibodies in Graves' diease (GD), but it remains unclear what the molecular basis of this pathological antibody response is. Stimulatory TSHR autoantibodies may induce activation of multiple signalling pathways in GD, modulate chemokine ...
Yang Y, Hui C.
europepmc   +2 more sources

Hybridoma technology: is it still useful?

Current Research in Immunology, 2021
The isolation of single monoclonal antibodies (mAbs) against a given antigen was only possible with the introduction of the hybridoma technology, which is based on the fusion of specific B lymphocytes with myeloma cells. Since then, several mAbs were described for therapeutic, diagnostic, and research purposes.
Jane Zveiter Moraes, Bárbara Hamaguchi, Camila Braggion, Enzo Reina Speciale, Fernanda Beatriz Viana Cesar, Gabriela de Fátima da Silva Soares, Juliana Harumi Osaki, Tauane Mathias Pereira, Rodrigo Barbosa Aguiar   +8 more
openaire   +4 more sources