Results 61 to 70 of about 45,417 (262)

Treg Cells Modulate Neuroinflammation and Behavioral Deficits in Autism: Evidence From MR‐Based Genetic Analyses and Experimental Models

American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, EarlyView.
ABSTRACT Autism spectrum disorder (ASD) is a neurodevelopmental condition that is increasingly linked to immune dysfunction and neuroinflammation. Regulatory T cells (Tregs), which are crucial in maintaining immune homeostasis, have been implicated in the pathogenesis of ASD.
Zuqing Nie, Xinyi Xu, Junhao Chen, Huiling Chen, Meng Li, Yang Zhang, Zhiwei Li, Chen Shen, Jie Wen, Xia Cao   +9 more
wiley   +1 more source

Cell growth cycle block of T cell hybridomas upon activation with antigen

Journal of Experimental Medicine, 1987
Stimulation of antigen-specific T cell hybridomas with the appropriate antigen/MHC combination, at concentrations that resulted in the secretion of the lymphokine interleukin 2, resulted in a dose-dependent decrease in both [3H]thymidine incorporation ...
J. Ashwell, R. E. Cunningham, P. Noguchi, D. Hernandez   +3 more
semanticscholar   +1 more source

Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody

Animal Research and One Health, EarlyView.
This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng, Xuyan Xiang, Xinyu Qi, Hanrong Zhou, Peiyu Xiao, Fandan Meng, Tongqing An, Haiwei Wang   +7 more
wiley   +1 more source

Lifelong evolution of autoreactive plasma cell numbers, affinity and anatomical location in arthritic K/BxN mice

Arthritis &Rheumatology, Accepted Article.
Objective The spontaneous K/BxN mouse model of rheumatoid arthritis has been used extensively to study chronic inflammation, contribution of immune cells, and the primordial role of autoreactive antibodies in disease initiation and severity. Only the ubiquitous enzyme glucose‐6‐phosphate isomerase (GPI) is the target of IgG autoantibodies secreted by ...
Thibault Vanhoucke, Carlos Castrillon, Odile Richard‐Le Goff, Angga Perima, Matteo Broketa, Andrew D. Griffiths, Patrick England, François Huetz, Pierre Bruhns   +8 more
wiley   +1 more source

Bottom‐Up Ice Growth Geometry Attenuates Shear Stress and Improves the Cryopreservation of Hematopoietic Stem/Progenitor Cells Under Low DMSO Concentrations

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Studies on cell cryopreservation have been limited by the complexity of the freezing process and challenges on controlling ice formation, managing cooling rates, and optimizing cryoprotectant concentrations. The objective of this study is to evaluate the impact of bottom‐up and conventional radial freezing on the viability of mammalian cells ...
Rafaela Ouro Neves, Pedro Sena Rego, Marta H. G. Costa, Isabel Bogalho, Andreia Duarte, Claúdia L. da Silva, Frederico Castelo Ferreira, Vitor Geraldes, Miguel A. Rodrigues   +8 more
wiley   +1 more source

Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor‐Like Performance

Biotechnology and Bioengineering, EarlyView.
This article presents the design and evaluation of a gas‐control jacket integrated with a breathable shake flask, enabling precise regulation of dissolved oxygen and carbon dioxide to improve scalability, process control, and culture performance in shaking bioreactors.
Vikash Kumar, Chad Sundberg, Venkatesh Srinivasan, Aaron Thole, Michael Tolosa, Govind Rao   +5 more
wiley   +1 more source

An automated microfluidic biosensor with bispecific rabbit monoclonal antibody and nanozyme‐bioenzyme composite for dual antibiotic detection

BMEMat, EarlyView.
This study introduces an automated microfluidic biosensor for simultaneous detection of florfenicol and thiamphenicol in environmental water. By integrating a phage‐screened bispecific rabbit monoclonal antibody with a nanozyme‐bioenzyme composite, the system achieves sensitive dual‐antibiotic detection. Coupled with smartphone‐based sensing, it offers
Fuyuan Zhang, Yang Liu, Juntao Wang, Haichao Zhang, Wenlong Yu, Yaxin Sang, Yun Liu, Guangze Yang, Fang Gao, Shuo Wang, Shilun Feng, Xianghong Wang   +11 more
wiley   +1 more source

Monoclonal Antibodies which Discriminate Between Native and Oxidized Low Density Lipoproteins

Revista Cubana de Investigaciones Biomédicas, 2020
Introduction: Atherosclerosis is a chronic inflammatory disorder and oxidation of low density lipoproteins (LDL) is thought to play a central role in atherogenesis.
Kenia María Rodríguez Oropesa, Yanín Bebelagua Cid, Ana María Vázquez   +2 more
doaj  

Dynamic pH profiles drive higher cell‐specific and volumetric productivity

Biotechnology Progress, EarlyView.
Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert, Dylan G. Chitwood, Danqia Peng, Erin Redman, Ji Young L. Anderson, Nicholas R. Sandoval, Sarah W. Harcum   +6 more
wiley   +1 more source

NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research [PDF]

, 2023
Keith G. Mitchell, Belvin Gong, Samuel S. Hunter, Diana Burkart‐Waco, Clara E. Gavira‐O'Neill, Kayla M. Templeton, Madeline E. Goethel, Malgorzata Bzymek, Leah M. MacNiven, Karl D. Murray, Matthew L. Settles, Lutz Froenicke, James S. Trimmer   +12 more
openalex   +1 more source