Results 81 to 90 of about 2,764,830 (288)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Regional Solutions coordinators

, 2017
"State resources are strategically aligned throughout Oregon to accomplish these goals [of community and economic development] though Regional Solutions Advisory Committees, Centers, and Teams...Coordination for these is provided by the Governor's Office"

core  

Loss of CYLD on Chromosome 16q Impairs Homologous Recombination and Genomic Stability Through TIRR Degradation

Advanced Science, EarlyView.
Chromosome 16q loss drives genomic instability through disruption of the CYLD–TIRR–53BP1 axis. CYLD preserves homologous recombination by stabilizing TIRR and limiting 53BP1 accumulation at DNA double‐strand breaks. CYLD deficiency redirects repair toward error‐prone non‐homologous end joining, promotes mutational burden and homologous recombination ...
Mingming Lu, Jialu Kang, Qi Ye, Zixi Wang, Lei Li, Yuzeshi Lei, Leihong Ye, Tianjie Liu, Bin Wang, Tao Liu, Shan Xu, Ke Wang, Yong Zhang, Jian Ma, Lei Li   +14 more
wiley   +1 more source

Hypotonic activation of the myo-inositol transporter SLC5A3 in HEK293 cells probed by cell volumetry, confocal and super-resolution microscopy.

PLoS ONE, 2015
Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. The present study explores the SLC5A3 protein as a possible transporter of myo-inositol in hyponically swollen ...
Joseph Andronic, Ryo Shirakashi, Simone U Pickel, Katherine M Westerling, Teresa Klein, Thorge Holm, Markus Sauer, Vladimir L Sukhorukov   +7 more
doaj   +1 more source

Intravenous postoperative fluid prescriptions for children: A survey of practice

BMC Surgery, 2008
Background Postoperative deaths and neurological injury have resulted from hyponatraemia associated with the use of hypotonic saline solutions following surgery.
Ali Tariq, Hall Tim, Davies Polly, Lakhoo Kokila   +3 more
doaj   +1 more source

THE BEHAVIOR OF FROG MUSCLE SPINDLE IN HYPER-AND HYPOTONIC SOLUTIONS

The Japanese Journal of Physiology, 1970
1. Isolated muscle spindles in the frog sartorius muscle were perfused in Ringer solutions made hypotonic by reducing NaCl or hypertonic by addition of NaCl, Na2SO4, Na2HPO4+NaH2PO4, sucrose, urea, glycerol, or propylene glycol.2. The frequency of spontaneous discharge at the in situ length of the muscle increased both in hypo- and hypertonic solutions.
openaire   +3 more sources

Targeting the NR1D1–IGF2BP2–V‐ATPase Axis With Hybrid Nanovesicles Restores Macrophage Rhythms to Reverse Sepsis‐Induced Immunosuppression

Advanced Science, EarlyView.
Sepsis disrupts immune‐cell rhythms and weakens bacterial clearance. Biomimetic nanovesicles combining erythrocyte and inflammation‐activated macrophage membranes deliver siNR1D1 to dysfunctional macrophages, restoring the NR1D1–IGF2BP2–V‐ATPase pathway, circadian regulation, phagolysosomal acidification, and antimicrobial defense.
Lang Chen, Wenyi Lin, Lang Jiang, Xuehui Gao, Chenyan Yu, Jili Han, Zhenping Liu, Xuefeng Li, Mei He, Chang Li, Qilan Li, Chaoying Song, Jiqian Xu, You Shang   +13 more
wiley   +1 more source

Exhaled nitric oxide after inhalation of isotonic and hypotonic solutions in healthy subjects.

, 2001
Airway nitric oxide (NO) homoeostasis is in ̄uenced by chemical and mechanical stimuli in humans; airway epithelium, which is an important site of NO production, is sensitive to osmotic challenge.
Sofia, M., Vatrella, A., Carratù L, VATRELLA, Alessandro, Cremona, G., Cremona G, Sofia M., Carratu, L., Maniscalco M, Maniscalco, M.   +9 more
core   +1 more source

Mesenchymal Stromal Cell‐Based Cell–Drug Conjugates for the Treatment of Acute Liver Failure

Advanced Science, EarlyView.
Mesenchymal stromal cell (MSC)‐based cell‐drug conjugates are constructed by anchoring rosiglitazone‐loaded nanoparticles onto the MSC surface. Sustained rosiglitazone release enhances MSC proliferation and paracrine activity while promoting anti‐inflammatory macrophage polarization.
Tenghui Ye, Jiamin Wu, Zixin Wu, Xi Liu, Jiajia Luo, Leyi Yang, Peng Shi   +6 more
wiley   +1 more source

Macrophage RSAD2 Couples mtDNA Synthesis With a Self‐Amplifying Inflammatory Circuit to Orchestrate Tissue Repair

Advanced Science, EarlyView.
Macrophage RSAD2 dually regulates CMPK2—the rate‐limiting enzyme for mitochondrial DNA synthesis—by suppressing its ubiquitination and promoting its phosphorylation via Csnk2a2 recruitment. This amplifies mtDNA production, which simultaneously activates a cGAS‐STING‐IRF3‐RSAD2 feedforward loop and the NLRP3 inflammasome, driving a self‐sustaining ...
Haomiao Yuan, Shukui Du, Chunfei Li, Xinjie Li, Shuyang Mu, Anran Qu, Hengxu Yan, Chongchong Xu, Jinnong Yang, Jingkai Sun, Yijun Wang, Jiayang Xiao, Fuyuan Zhang, Linlin Wang, Tiegang Li, Rui Zhao, Dawei Guan   +16 more
wiley   +1 more source