Results 91 to 100 of about 14,819 (239)

Targeted Nucleotide Substitution in Mammalian Cell by Target-AID

Bio-Protocol, 2017
Programmable RNA-guided nucleases based on CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) systems have been applied to various type of cells as powerful genome editing tools. By using activation-induced
Takayuki Arazoe, Keiji Nishida, Akihiko Kondo   +2 more
doaj   +1 more source

Uric Acid‐Lowering and Anti‐Hyperuricemia Effects of Plant‐Based Nutraceuticals and Foods: A Review

Food Chemistry International, Volume 1, Issue 3, Page 216-250, September 2025.
Increased UA production and/or decreased UA secretion cause hyperuricemia. Effects and mechanism of flavonoids in reducing UA are summarized. Terpenoids, saponins, alkaloids, vitamin C, polysaccharides, peptides are effective. Plant‐based foods with UA‐lowering ability are reviewed.
Linwei He, Wenjian Yang, Jianhui Liu
wiley   +1 more source

Data supporting the design and evaluation of a universal primer pair for pseudogene-free amplification of HPRT1 in real-time PCR

Data in Brief, 2015
Hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) is a common housekeeping gene for sample normalization in the quantitative reverse transcriptase polymerase chain (qRT-PCR).
Reza Valadan, Akbar Hedayatizadeh-Omran, Mahdyieh Naghavi Alhosseini-Abyazani, Omolbanin Amjadi, Alireza Rafiei, Mohsen Tehrani, Reza Alizadeh-Navaei   +6 more
doaj   +1 more source

Leishmania promastigotes evade interleukin 12 (IL-12) induction by macrophages and stimulate a broad range of cytokines from CD4+ T cells during initiation of infection. [PDF]

, 1994
Leishmania major are intramacrophage parasites whose eradication requires the induction of T helper 1 (Th1) effector cells capable of activating macrophages to a microbicidal state.
Locksley, RM, Reiner, SL, Stowring, L, Wang, ZE, Zheng, S   +4 more
core   +1 more source

Sex‐Specific Long‐Term Effects of Perinatal Limosilactobacillus reuteri on Social Cognition, Gene Expression, and Gut Microbiota

Journal of Neurochemistry, Volume 169, Issue 8, August 2025.
Perinatal supplementation with Limosilactobacillus reuteri (L. reuteri) during gestation and early postnatal life induces long‐lasting, sex‐specific effects on the microbiota–gut–brain axis. Treated male offspring showed enhanced sociability, social recognition, microbial shifts, and coordinated transcriptional changes across the brain and gut.
Tatiana Siegler Lathrop, Inés Martínez Sanchez, Ioannis S. Chronakis, Rochellys Diaz Heijtz   +3 more
wiley   +1 more source

Immunization with purine salvation pathway recombinant enzymes induces the production of anti- Schistosoma mansoni immunoglobulines [PDF]

Schistosomiasis, a disease caused by the trematodes of the Schistosoma genus, affects about 240 million people worldwide. The Praziquantel (PZQ) is the drug used to treat this disease. However, reports of resistant strains reinforce the need to develop a
Adachi, Felipe de Pontes, Anibal, Fernanda de Freitas, Correia, Ricardo de Oliveira, Dejani, Naiara Naiana, Garratt, Richard Charles, Neris, Débora Meira, Oliveira, Sandra Regina Pereira de, Pereira, Humberto D'Muniz, Rodolpho, Joice Margareth de Almeida, Rodrigues, Vanderlei, Souza, Laís Cristina de   +10 more
core  

The antiepileptic potential of nucleosides [PDF]

, 2014
Despite newly developed antiepileptic drugs to suppress epileptic symptoms, approximately one third of patients remain drug refractory. Consequently, there is an urgent need to develop more effective therapeutic approaches to treat epilepsy. A great deal
Dobolyi, Á., Juhász, G., Kovács, Z., Kékesi, K .A.   +3 more
core   +1 more source

Differential changes in anatomical, molecular and functional determinants of intestinal glucose absorption during murine pregnancy

Physiological Reports, Volume 13, Issue 15, August 2025.
Abstract Pregnancy demands increased food intake and nutrient delivery to support the growing conceptus. The timing and regional specificity of adaptations in small intestinal (SI) anatomy and transport, and their functional consequences, remain unclear. We therefore assessed anatomy and expression of macronutrient transporters in the duodenum, jejunum,
T. Sebastian Overduin, Georgia S. Clarke, Hui Li, Richard L. Young, Kathryn L. Gatford, Amanda J. Page   +5 more
wiley   +1 more source

Interference of Sulphonate Buffering Agents with E. coli Hypoxanthine-Guanine Phosphoribosyltransferase Active Site Functioning: A Crystallographic and Enzymological Study

Crystals
The investigation of the structure–function relationship in hypoxanthine-guanine phosphoribosyltransferases (HGPRT) is a direction that is relevant for the development of drugs and approaches of enzymatic synthesis of modified nucleosides and nucleotides.
Evgeniy A. Zayats, Yulia A. Abramchik, Maria A. Kostromina, Vladimir I. Timofeev, Mikhail B. Shevtsov, Alexey V. Mishin, Ilya V. Fateev, Andrey A. Karanov, Alexandra R. Sharafutdinova, Aleksandra O. Arnautova, Irina D. Konstantinova, Valentin I. Borshchevskiy, Roman S. Esipov   +12 more
doaj   +1 more source

Metabolic and neurobehavioral disturbances induced by purine recycling deficiency in Drosophila

eLife
Adenine phosphoribosyltransferase (APRT) and hypoxanthine-guanine phosphoribosyltransferase (HGPRT) are two structurally related enzymes involved in purine recycling in humans.
Céline Petitgas, Laurent Seugnet, Amina Dulac, Giorgio Matassi, Ali Mteyrek, Rebecca Fima, Marion Strehaiano, Joana Dagorret, Baya Chérif-Zahar, Sandrine Marie, Irène Ceballos-Picot, Serge Birman   +11 more
doaj   +1 more source