Results 181 to 190 of about 9,789 (207)
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A spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1971Abstract The present paper describes a new spectrophotometric assay for HGPRTase activity which is more rapid than and as sensitive as the isotopic assays for this enzyme and which avoids the use of high-voltage electrophoresis and liquid scintillation counting. A simple technique using thin-layer chromatography for the separation of the nucleotide,
David S. Newcombe, James M. Willard
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Biochemical Medicine, 1973
Abstract Hypoxanthine transport has been studied in cultured human fibroblasts with normal and mutant H-G PRT. Transport is dependent on cell density, the activity of H-G PRT, and de novo purine synthesis. Transport is decreased in control cultures at high density and in cell strains with a mutant H-G PRT.
Norma Herrick +2 more
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Abstract Hypoxanthine transport has been studied in cultured human fibroblasts with normal and mutant H-G PRT. Transport is dependent on cell density, the activity of H-G PRT, and de novo purine synthesis. Transport is decreased in control cultures at high density and in cell strains with a mutant H-G PRT.
Norma Herrick +2 more
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Hypoxanthine Phosphoribosyltransferase and Hypoxanthine Uptake in Human Erythrocytes
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1975A system of hypoxanthine uptake and IMP retention was studied and characterized in human erythrocytes. It follows closely the system already described for rabbit erythrocytes[7]. IMP formation and retention are dependent on the activity of hypoxanthine phosphoribosyl-transferase and on intracellular availability of phosphoribosyl pyrophosphate (P-Rib ...
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International Journal of Biochemistry, 1986
A rapid and simple method, based on GMP Sepharose affinity chromatography, was used for the purification of human brain hypoxanthine guanine phosphoribosyltransferase. A single protein band was detected by polyacrylamide gel electrophoresis of the native purified enzyme. A subunit molecular weight of 25,000 was estimated by SDS gel electrophoresis. The
Shigeki Nakagawa +3 more
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A rapid and simple method, based on GMP Sepharose affinity chromatography, was used for the purification of human brain hypoxanthine guanine phosphoribosyltransferase. A single protein band was detected by polyacrylamide gel electrophoresis of the native purified enzyme. A subunit molecular weight of 25,000 was estimated by SDS gel electrophoresis. The
Shigeki Nakagawa +3 more
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Immunoadsorbent Chromatography of Hypoxanthine-Guanine Phosphoribosyltransferase
1974Recent evidence indicates that the virtual absence of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in patients with the Lesch-Nyhan syndrome is due in most if not all instances to a mutation(s) on the gene coding for the HGPRT protein (Kelley and Meade, 1971; Rubin, et al., 1971; Arnold, Meade and Kelley, 1972).
R. B. Jones +2 more
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Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Humangenetik, 1974A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan.
Someren, H. van +2 more
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A continuous spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a simple and rapid spectrophotometric assay of hypoxanthine-guanine phosphoribosyltransferase based on the continuous monitoring of product concentration by a NADH-coupled enzyme system.
GIACOMELLO, Alessandro +1 more
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Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae
Molecular and Biochemical Parasitology, 1987Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was isolated from the malarial parasite, Plasmodium lophurae. The apparent pI, as determined by chromatofocusing, was 7.6. The native molecular weight was 79,000. The pH profile of HGPRT exhibited a broad pH optimum. With hypoxanthine as substrate maximal activity was achieved from pH 6.0-10.0,
Larry A. Mole +2 more
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Evidence for tetrameric structure of mammalian hypoxanthine phosphoribosyltransferase
Biochemical Genetics, 1987A fast electrophoretic variant of hypoxanthine phosphoribosyltransferase (HPRT) has been detected in Mus musculus bactrianus, a mouse subspecies from Middle Asia (USSR). The electrophoretic HPRT pattern yielded by hybrids between the somatic cell of LMTK- (deficient in thymidine kinase) and the splenocytes of a male of M. m. bactrianus was five-banded.
Suren M. Zakian +5 more
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Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay
Clinica Chimica Acta, 1977A simple spectrophotometric assay is described based on the conversion of hypoxanthine to inosine monophosphate and precipitation of both the reaction product and protein with lanthanum phosphate. The extent of conversion is determined by the fall in absorbance of hypoxanthine at 249 nm.
Johnson L.A., Gordon R.B., Emmerson B.T.
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