Results 181 to 190 of about 13,871 (203)
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Immunoadsorbent Chromatography of Hypoxanthine-Guanine Phosphoribosyltransferase
1974Recent evidence indicates that the virtual absence of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in patients with the Lesch-Nyhan syndrome is due in most if not all instances to a mutation(s) on the gene coding for the HGPRT protein (Kelley and Meade, 1971; Rubin, et al., 1971; Arnold, Meade and Kelley, 1972).
R. B. Jones +2 more
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A continuous spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a simple and rapid spectrophotometric assay of hypoxanthine-guanine phosphoribosyltransferase based on the continuous monitoring of product concentration by a NADH-coupled enzyme system.
GIACOMELLO, Alessandro +1 more
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Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae
Molecular and Biochemical Parasitology, 1987Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was isolated from the malarial parasite, Plasmodium lophurae. The apparent pI, as determined by chromatofocusing, was 7.6. The native molecular weight was 79,000. The pH profile of HGPRT exhibited a broad pH optimum. With hypoxanthine as substrate maximal activity was achieved from pH 6.0-10.0,
Larry A. Mole +2 more
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Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Humangenetik, 1974A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan.
P. Meera Khan +2 more
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Hypoxanthine: Guanine phosphoribosyltransferase mutants in Saccharomyces cerevisiae
Molecular and General Genetics MGG, 1983Yeast mutants lacking activity of the enzyme hypoxanthine:guanine phosphoribosyltransferase (H:G-PRT) have been isolated by selecting for resistance to 8-azaguanine in a strain carrying the wild type allele, ade4%, of the gene coding for amidophosphoribosyltransferase (PRPPAT), the first enzyme of de novo purine synthesis.
Darlene G. Roberts +4 more
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Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay
Clinica Chimica Acta, 1977A simple spectrophotometric assay is described based on the conversion of hypoxanthine to inosine monophosphate and precipitation of both the reaction product and protein with lanthanum phosphate. The extent of conversion is determined by the fall in absorbance of hypoxanthine at 249 nm.
Johnson L.A., Gordon R.B., Emmerson B.T.
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Evidence for tetrameric structure of mammalian hypoxanthine phosphoribosyltransferase
Biochemical Genetics, 1987A fast electrophoretic variant of hypoxanthine phosphoribosyltransferase (HPRT) has been detected in Mus musculus bactrianus, a mouse subspecies from Middle Asia (USSR). The electrophoretic HPRT pattern yielded by hybrids between the somatic cell of LMTK- (deficient in thymidine kinase) and the splenocytes of a male of M. m. bactrianus was five-banded.
Suren M. Zakian +5 more
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The Spectrum of Hypoxanthine-Guanine Phosphoribosyltransferase (HPRT) Deficiency
2006Summary The enzyme hypoxanthine-guanine phosphoribo-syltransferase (HPRT) catalyzes the reutilization ofhypoxanthine and guanine to the purine nucleotidesIMP and GMP, respectively. HPRT deficiency is an X-linked disorder characterized by uric acid over-production and variable neurologic impairment.
Rosa J. Torres +5 more
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Levels of hypoxanthine phosphoribosyltransferase RNA in human cells
Experimental Cell Research, 1990The gene for the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT) is expressed at a low level in many cells. As is the case with several other "housekeeping genes," thorough studies of hprt gene regulation have been hampered by the low levels of its mRNA.
Dennis Hellgren +3 more
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A rapid radiochemical assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1973Abstract A simple radiochemical method is described for assay of hypoxanthine-guanine phosphoribosyltransferase. 14C-Hypoxanthine is incubated with enzyme PRPP. The labelled product is precipitated on strips of Whatman No. 1 paper by the addition of lanthanum nitrate. Unreacted substrate is eluted with distilled water.
Vincent P. Hollander +1 more
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