Results 261 to 270 of about 504,672 (298)

Guidelines for accurate EC50/IC50 estimation

Pharmaceutical Statistics, 2011
AbstractThis article provides minimum requirements for having confidence in the accuracy of EC50/IC50 estimates. Two definitions of EC50/IC50s are considered: relative and absolute. The relative EC50/IC50 is the parameter c in the 4‐parameter logistic model and is the concentration corresponding to a response midway between the estimates of the lower ...
exaly   +3 more sources

An IC 50 Calculator: brittalundstroem/IC50: IC50 R tool: Dominic; Marrnel

2023
An R Shiny App to calculate IC50 and confidence interval.
Ritler, Dominic, Marreros, Nelson, Lundström-Stadelmann, Britta   +2 more
openaire   +1 more source

DFT based Computational Methodology of IC50 Prediction

Current Computer-Aided Drug Design, 2021
Background: IC 50 is one of the most important parameters of a drug. But, it is very difficult to predict this value of a new compound without experiment. There are only a few QSAR based methods available for IC 50
openaire   +2 more sources

Relationships between Inhibition Constants, Types of Inhibition and IC50: Calculation and Comparison on IC50 of Two Model Tyrosinase Inhibitors

2010 4th International Conference on Bioinformatics and Biomedical Engineering, 2010
Calculating methods for inhibitor concentration (IC50) leading to 50% activity lost (for diphenolase activity) of a novel parabolic competitive inhibitor (the trifluoromethyl-containing 1,2,3-triazole, TF-TA) and a novel mixed inhibitor ([E]-4-(1-imidazoylmethyl) cinnamic acid (ozagrel) were derived on the basis of kinetics of tyrosinase inhibition ...
Hairong Mao, Jiangang Xie, Qimeng Zhang, Xinyu Lu, Shubai Li   +4 more
openaire   +1 more source

An Examination of IC50 and IC50-Shift Experiments in Assessing Time-Dependent Inhibition of CYP3A4, CYP2D6 and CYP2C9 in Human Liver Microsomes

Drug Metabolism Letters, 2008
The relationship between time-dependent inactivation (TDI) and IC50 is examined using a consolidated method for evaluating CYP450 inhibition during drug discovery. An IC50 fold-shift of >1.5 indicated significant TDI potency. Further, the "shifted IC50" could be used to estimate, the K(I) and TDI potency ratio k(inact)/K(I) to within 2-fold in most ...
Loren M, Berry, Zhiyang, Zhao
openaire   +2 more sources

The IC50 Concept Revisited

Current Topics in Medicinal Chemistry, 2012
A major strategy used in drug design is the inhibition of enzyme activity. The ability to accurately measure the concentration of the inhibitor which is required to inhibit a given biological or biochemical function by half is extremely important in ranking compounds.
Gary W, Caldwell, Zhengyin, Yan, Wensheng, Lang, John A, Masucci   +3 more
openaire   +2 more sources

Impact of Impurities on IC50 Values of P450 Inhibitors

Drug Metabolism Letters, 2011
During early drug discovery, the synthetic pathways for test compounds are not well defined and impurities in the test compounds are inevitable. Compounds undergo serial screening tests at this stage to assess their biological activities and drug-like properties.
openaire   +2 more sources

Measuring Bioactivity: KI, IC50 and EC50

2011
Which quantity permits a characterisation of the performance of a bioactive molecule? How can a test be created so as to detect the effect of a molecule on a given target? Are there any general rules to respect? The design of a test is a complex problem dealt with in chapter 3; here we emphasise that, above all, the target must be a limiting factor in ...
openaire   +2 more sources

CURCUMIN HAS LOWER IC50 VALUES AGAINST A549 LUNG CANCER CELLS

2023
AbstractIn this study, we aimed to determine the toxic effect of curcumin on A549 lung cancer cells and to show how this effect is reflected in morphology. Firstly, the toxic doses of curcumin against A549 lung cancer cells were determined via MTT and neutral red cytotoxicity tests by using the doses of 6.25, 12.5, 25, 50, 100, 200 and 400 µM.
Sedat Kaçar, Özlem Tomsuk, Varol Şahintürk   +2 more
openaire   +1 more source