Results 81 to 90 of about 194,023 (268)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

The significant influence of the Atlantic multidecadal variability to the abrupt warming in Northeast Asia in the 1990s

npj Climate and Atmospheric Science
Northeast Asia experienced unprecedented abrupt warming in the 1990s since the last century. Based on a robust time series and rank frequency evaluation, the Max Planck Institute for Meteorology Grand Ensembles of CMIP5 (MPI-GE5), CMIP6 (MPI-GE6), EC ...
Kaiwen Zhang, Zhiyan Zuo, Laura Suarez-Gutierrez, Lulei Bu   +3 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Ice Model Tests for Dynamic Positioning Vessel in Managed Ice

Arctic Technology Conference, 2016
Abstract Stationkeeping in managed ice using dynamic positioning (DP) control system has been an area of great interest over the past few years. The stationkeeping performance of a DP vessel depends on the modelling accuracy of the ice forces, which in turn depends on managed ice field characteristics (floe size, floe thickness ...
Wang, Jungyong, Sayeed, Tanvir, Millan, David, Gash, Robert, Islam, Mohammed, Millan, James   +5 more
openaire   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Changes in ice dynamics and mass balance of the Antarctic ice sheet [PDF]

Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2006
The concept that the Antarctic ice sheet changes with eternal slowness has been challenged by recent observations from satellites. Pronounced regional warming in the Antarctic Peninsula triggered ice shelf collapse, which led to a 10-fold increase in glacier flow and rapid ice sheet retreat.
openaire   +4 more sources

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Deep Neural Network Based Scale‐Adaptive Cloud Vertical Overlap Parameterization

Geophysical Research Letters
Cloud vertical overlap has important implications for cloud radiative effects in climate models. Due to its spatiotemporal complexity, it is difficult to simulate cloud overlap in climate models.
Xin Wu, Feng Zhang, Hua Zhang, Haixia Xiao, Guoxing Chen   +4 more
doaj   +1 more source

Diverse behaviors of marine ice sheets in response to temporal variability of the atmospheric and basal conditions

Journal of Glaciology
The observed retreat of the grounding line of the present-day ice sheets and the simulated grounding-line retreat of ice sheets under changing climate conditions are often interpreted as indications of marine ice-sheet instability (MISI), driven by a ...
Olga Sergienko, Duncan John Wingham
doaj   +1 more source

Dynamical response of the southwestern Laurentide Ice Sheet to rapid Bølling–Allerød warming [PDF]

The Cryosphere
The shift in climate that occurred between the Last Glacial Maximum (LGM) and the Early Holocene (ca. 18–12 kyr BP) displayed rates of temperature increase similar to present-day warming trends.
S. L. Norris, S. L. Norris, M. Margold, D. J. A. Evans, N. Atkinson, D. G. Froese   +5 more
doaj   +1 more source