Results 81 to 90 of about 453,669 (254)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

DESEMPENHO DE FRANGOS DE CORTE, SEXADOS, SUBMETIDOS A DIETAS FORMULADAS PELOS CONCEITOS DE PROTEÍNA BRUTA versus PROTEÍNA IDEAL PERFORMANCE OF SEXED BROILER BIRDS, SUBMITTED TO DIETS FORMULATED BY THE CONCEPTS OF CRUDE PROTEIN versus IDEAL PROTEIN

Ciência Rural, 2001
O trabalho foi conduzido no Setor de Avicultura do Departamento de Zootecnia da Universidade Federal de Santa Maria com o objetivo de verificar as respostas de desempenho de frangos de corte, criados com sexos separados, alimentados pelo conceito ...
Maria Olinda Bergamo Mendoza, Paulo Tabajara Chaves Costa, Lia Helena Katzer, Antonio Carlos Benetti, Zenita Bisognin Santi, José Nelson Welter   +5 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Ideal Glasses and Protein Network Dynamics

, 2005
Quantitative topological analogies between the flexibilities of optimized inorganic glasses, small biological molecules, and proteins suggest that mean field estimates of internal stress are useful in identifying mechanisms supporting living materials and their enzymatic products.
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effect of ideal protein versus low-fat diet for weight loss: A randomized controlled trial. [PDF]

Obes Sci Pract, 2022
Fernandez CA, Potts K, Bazzano LA.
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Comparison of Different Ideal Amino Acid Ratios in Male and Female Broiler Chickens of 21 to 42 Days of Age

The Journal of Poultry Science, 2008
An amino acid bioassay was carried out to compare responses of male and female broiler chicks to different ideal amino acid (AA) ratios from 21 to 42d of age.
Reza Taherkhani, Mahmood Shivazad, Mojtaba Zaghari, Ahmad Zare Shahneh   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Growth Responses of Broiler Chicks to Different Levels of Threonine and Tryptophan in Chemically-Defined Diets

The Journal of Poultry Science, 2007
In Experiment 1 three semi-purified diets were formulated using isolated soy protein and crystalline amino acids (AA) to contain three profiles of essential amino acids (EAA) based on NRC (1994; Diet 1), Illinois Ideal Chick Protein (IICP; Diet 2) and ...
Robert Blair, Chen H. Chong, Sazzad M. Hossain   +2 more
doaj   +1 more source