Results 71 to 80 of about 1,964,998 (260)

Seed Germination Mechanism of Carex rigescens Under Variable Temperature Determinded Using Integrated Single-Molecule Long-Read and Illumina Sequence Analysis. [PDF]

Front Plant Sci, 2022
Li H, Teng K, Yue Y, Teng W, Zhang H, Wen H, Wu J, Fan X.   +7 more
europepmc   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing.
S. Nouws, S.C.J. De Keersmaecker, K. Vanneste, N. Roosens, B. Bogaerts, S. Hoffman, A. Van den Bossche, B. Verhaegen, M. Godfroid, L. Delbrassinne, W. Mattheus, Vden Bossch An   +11 more
core   +1 more source

Summary of Illumina transcriptome sequence data and quality control.

, 2014
* percentage of reads left after trimming and filtering.Summary of Illumina transcriptome sequence data and quality control.
Ailton B. Santa Brígida (671969), Lívia Vargas (671968), José P. Mota Filho (671970), Michiel Van Bel (671974), Klaas Vandepoele (23662), Adriana S. Hemerly (671976), Thais G. de Carvalho (671971), Paulo C. G. Ferreira (605671), Laurent Farrinelli (671975), Cristian A. Rojas (671972), Dries Vaneechoutte (671973)   +10 more
core   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis [PDF]

, 2010
Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous fungi have been sequenced, most of them by Sanger sequencing.
Kück Ulrich, Espagne, Eric, Jason E Stajich, Kempken, Frank, Ines Engh, Stefanie Pöggeler, Frank Kempken, Ulrich Kück, Kuo, Hsiao Che, Jens Kamerewerd, Osiewacz, Heinz D., Zickler, Denise, Espagne Eric, Kück, Ulrich, Engh Ines, Freitag, Michael H., Smith, Kristina M, Meiling Chu, Chu Meiling, Smith Kristina M., Kuo, Hsiao-Che, Read, Nick D, Heinz D Osiewacz, Kempken Frank, Poeggeler, Stefanie, Knab, Birgit, Birgit Knab, Denise Zickler, Kuo Hsiao-Che, Kueck, Ulrich, Stajich Jason E., Osiewacz, Heinz D, Stajich, Jason E, Michael Freitag, Stephan Seiler, Halliday, Karen, Freitag, Michael, Stajich, Jason E., Chu, Meiling, Nick D Read, Karen Halliday, Knab Birgit, Zickler Denise, Kristina M Smith, Pöggeler, Stefanie, Engh, Ines, Eric Espagne, Hsiao-Che Kuo, Kamerewerd Jens, Smith, Kristina M., Halliday Karen, Pöggeler Stefanie, Seiler Stephan, Read Nick D., Nowrousian, Minou, Seiler, Stephan, Osiewacz Heinz D., Freitag Michael, Nowrousian Minou, Read, Nick D., Kamerewerd, Jens, Minou Nowrousian   +61 more
core   +2 more sources

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Characterizing the mouse ES cell transcriptome with Illumina sequencing

Genomics, 2008
Large datasets generated by Illumina sequencing are ideally suited to transcriptome characterization. We generated 3,052,501 27-mer reads from F1 mouse embryonic stem (ES) cell cDNA. Using the ELAND alignment tool, 74.5% of reads matched sequenced mouse resources,
Rosenkranz, R., Borodina, T., Lehrach, H., Himmelbauer, H.   +3 more
openaire   +3 more sources

Plasmodium knowlesi Genome Sequences from Clinical Isolates Reveal Extensive Genomic Dimorphism. [PDF]

, 2015
Plasmodium knowlesi is a newly described zoonosis that causes malaria in the human population that can be severe and fatal. The study of P. knowlesi parasites from human clinical isolates is relatively new and, in order to obtain maximum information from
Sanderson, Theo, Scott B Millar, Theo, Sanderson, Janet Cox-Singh (218129), Janet Cox-Singh, Rayner, Julian C., Rayner, Julian C, Janet, Cox-Singh, Millar, SB, Sanderson Theo, Scott B. Millar (615592), Sanjeev Krishna (4917), Miguel M. Pinheiro (615585), Miguel M., Pinheiro, Pinheiro, MM, Sanjeev, Krishna, Ahmed, Md Atique, Lu Woon Chan, Millar Scott B., Md Atique Ahmed, Sanjeev Krishna, Monsanto Pinheiro, Miguel, Thomas, D. Otto, Millar, Scott B, Pinheiro Miguel M., Lu, Woon Chan, Thomas D. Otto (188722), Cox-Singh, J, Md Atique, Ahmed, Rayner Julian C., Theo Sanderson (5662720), Cox-Singh, Janet, Miguel M Pinheiro, Otto, Thomas D, Otto, TD, Otto, Thomas D., Scott B., Millar, Millar, Scott B., Cox-Singh Janet, Krishna Sanjeev, Lu, WC, Ahmed Md Atique, Julian C Rayner, Woon, Chan Lu, Woon Chan Lu (716048), Julian C., Rayner, Md Atique Ahmed (716047), Sanderson, T, Krishna, S, Krishna, Sanjeev, Otto Thomas D., Theo Sanderson, Rayner, JC, Pinheiro, Miguel M., Ahmed, MA, Julian C. Rayner (121198), Thomas D Otto, Woon Chan Lu   +57 more
core   +2 more sources