Systematic Comparison of Nanopore and Illumina Sequencing for the Detection of Plant Viruses and Viroids Using Total RNA Sequencing Approach [PDF]
Frontiers in Microbiology, 2022 High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Ion Gutiérrez-aguirre +2 more
exaly +5 more sources
Sequencing error profiles of Illumina sequencing instruments [PDF]
NAR Genom Bioinform, 2021 Sequencing technology has achieved great advances in the past decade. Studies have previously shown the quality of specific instruments in controlled conditions.
Nicholas Stoler, A. Nekrutenko
semanticscholar +5 more sources
Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]
PLoS ONE, 2014 The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
A Adey +18 more
core +22 more sources
Comparison of illumina and 454 deep sequencing in participants failing raltegravir-based antiretroviral therapy. [PDF]
PLoS ONE, 2014 The impact of raltegravir-resistant HIV-1 minority variants (MVs) on raltegravir treatment failure is unknown. Illumina sequencing offers greater throughput than 454, but sequence analysis tools for viral sequencing are needed.
Jonathan Z Li +30 more
doaj +8 more sources
Assembling the perfect bacterial genome using Oxford Nanopore and Illumina sequencing [PDF]
PLoS Comput Biol, 2023 A perfect bacterial genome assembly is one where the assembled sequence is an exact match for the organism’s genome—each replicon sequence is complete and contains no errors. While this has been difficult to achieve in the past, improvements in long-read
R. Wick, L. Judd, K. Holt
semanticscholar +2 more sources
A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay. [PDF]
PLoS ONE, 2023 Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require
Jason D Limberis +3 more
doaj +4 more sources
Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination [PDF]
, 2011 Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3-5-kb DNA fragment are not easy to identify and pose ...
Deforce, Dieter +6 more
core +4 more sources
Highly comparable metabarcoding results from MGI-Tech and Illumina sequencing platforms [PDF]
PeerJ, 2021 With the developments in DNA nanoball sequencing technologies and the emergence of new platforms, there is an increasing interest in their performance in comparison with the widely used sequencing-by-synthesis methods.
Sten Anslan +8 more
semanticscholar +2 more sources
Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing [PDF]
PLoS One, 2021 Pollen metabarcoding has received much attention recently for its potential to increase taxonomic resolution of the identifications of pollen grains necessary for various public health, ecological and environmental inquiry.
S. Swenson, B. Gemeinholzer
semanticscholar +2 more sources
Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
Nature Methods, 2012 High-throughput sequencing has revolutionized microbial ecology, but read quality remains a considerable barrier to accurate taxonomy assignment and α-diversity assessment for microbial communities.
Nicholas A. Bokulich +7 more
openalex +3 more sources