Results 31 to 40 of about 599,955 (287)

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens. [PDF]

J Clin Microbiol
Bogaerts B, Van den Bossche A, Verhaegen B, Delbrassinne L, Mattheus W, Nouws S, Godfroid M, Hoffman S, Roosens NHC, De Keersmaecker SCJ, Vanneste K.   +10 more
europepmc   +2 more sources

Reference genome and comparative genome analysis for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine [PDF]

, 2019
Background: Mycobacterium bovis bacillus Calmette-Guerin (M. bovis BCG) is the only vaccine available against tuberculosis (TB). In an effort to standardize the vaccine production, three substrains, i.e. BCG Danish 1331, Tokyo 172-1 and Russia BCG-1 were
Borgers, Katlyn, Callewaert, Nico, Festjens, Nele, Lin, Yao-Cheng, Michielsen, Gitte, Ou, Jheng-Yang, Plets, Evelyn, Tiels, Petra, Van Hecke, Annelies, Zheng, Po-Xing   +9 more
core   +2 more sources

Chromosome-level haplotype-resolved genome assembly for Takifugu ocellatus using PacBio and Hi-C technologies

Scientific Data, 2023
Measurement(s) Whole Genome Sequencing • Chromosome assembly by Hi-C data • Whole Transcriptome Sequencing Technology Type(s) PacBio Sequel System • Hi-C • Illumina HiSeq. 2500 • Illumina NovaSeq.
Qingmin Zeng, Zhixiong Zhou, Qian He, Leibin Li, Fei Pu, Mengzhen Yan, Peng Xu   +6 more
doaj   +1 more source

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies [PDF]

, 2014
Background: Cost effective next generation sequencing technologies now enable the production of genomic datasets for many novel planktonic eukaryotes, representing an understudied reservoir of genetic diversity. O.
Blanc-Mathieu, Romain, Bouget, François-Yves, Carré, Isabelle A., Château, Annie, Derelle, Evelyne, Eyre-Walker, Adam, Grimsley, Nigel, Moreau, Hervé, Peer, Yves van de, Piganeau, Gwenaël, Piégu, Benoit, Rivals, Eric, Rombauts, Stephane, Schackwitz, Wendy, Verhelst, Bram   +14 more
core   +6 more sources

A pilot study for channel catfish whole genome sequencing and de novo assembly

BMC Genomics, 2011
Background Recent advances in next-generation sequencing technologies have drastically increased throughput and significantly reduced sequencing costs.
Jiang Yanliang, Lu Jianguo, Peatman Eric, Kucuktas Huseyin, Liu Shikai, Wang Shaolin, Sun Fanyue, Liu Zhanjiang   +7 more
doaj   +1 more source

Comparison of the Illumina NextSeq 2000 and GeneMind Genolab M sequencing platforms for spatial transcriptomics

BMC Genomics, 2023
Background The Illumina sequencing systems demonstrate high efficiency and power and remain the most popular platforms. Platforms with similar throughput and quality profiles but lower costs are under intensive development. In this study, we compared two
Iamshchikov Pavel, Larionova Irina, Gerashchenko Tatiana, Piankov Denis, Koshkin Philipp, Korostelev Sergei, Denisov Evgeny   +6 more
doaj   +1 more source

Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]

, 2018
In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini, Campanaro, Stefano, Kougias, Panagiotis G., Treu, Laura, Zhu, Xinyu   +4 more
core   +3 more sources

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

A single polyploidization event at the origin of the tetraploid genome of Coffea arabica is responsible for the extremely low genetic variation in wild and cultivated germplasm [PDF]

, 2020
The genome of the allotetraploid species Coffea arabica L. was sequenced to assemble independently the two component subgenomes (putatively deriving from C. canephora and C.
Al Hakimi, Amin, Bentley, Nolan, Bertrand, Benoît, Cattonaro, Frederica, Cerutti, Mario, Del Terra, Lorenzo, Di Gaspero, Gabriele, Graziosi, Giorgio, Jurman, Irena, Klein, Patricia E., Magni, Federica, Magris, Gabriele, Montagnon, Christophe, Morgante, Michele, Murray, Seth C., Navarini, Luciano, Pallavicini, Alberto, Pellegrino, Gloria, Pinosio, Sara, Ruosi, Manuela Rosanna, Scaglione, Davide, Scalabrin, Simone, Schilling, Timothy, Solano, William, Suggi Liverani, Furio, Toniutti, Lucile, Valle, Giorgio, Vidotto, Michele, Vitulo, Nicola   +28 more
core   +3 more sources