Results 31 to 40 of about 463,626 (303)

PANDAseq: paired-end assembler for illumina sequences [PDF]

BMC Bioinformatics, 2012
Abstract Background Illumina paired-end reads are used to analyse microbial communities by targeting amplicons of the 16S rRNA gene. Publicly available tools are needed to assemble overlapping paired-end reads while correcting mismatches and uncalled bases; many errors could be corrected to obtain higher sequence ...
Andre P. Masella, Andrea K. Bartram, Jakub Truszkowski, Daniel Gregory Brown, Josh D. Neufeld   +4 more
openaire   +3 more sources

Short read Illumina data for the de novo assembly of a non-model snail species transcriptome (Radix balthica, Basommatophora, Pulmonata), and a comparison of assembler performance [PDF]

, 2011
Background: Until recently, read lengths on the Solexa/Illumina system were too short to reliably assemble transcriptomes without a reference sequence, especially for non-model organisms.
Wheat, Christopher W., Markus Pfenninger, Rotter, Björn, Pfenninger, Markus, Björn Rotter, Christopher W Wheat, Nicolas Krezdorn, Feldmeyer, Barbara, Krezdorn, Nicolas, Rotter, Bjoern, Barbara Feldmeyer   +10 more
core   +1 more source

Illumina Sequencing Library Construction from ChIP DNA

Bio-Protocol, 2012
The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive.
Wei Zheng
doaj   +1 more source

Special features of RAD Sequencing data:implications for genotyping [PDF]

, 2012
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Fuentes-Utrilla, Pablo, Blaxter, Mark L, Pablo Fuentes-utrilla, Karim Gharbi, Davey, John W, Cezard, Timothée, John W. Davey, Gharbi, Karim, Pablo Fuentes‐Utrilla, Cathlene Eland, Timothée Cezard, Mark L. Blaxter, Eland, Cathlene   +12 more
core   +1 more source

Paragraph: a graph-based structural variant genotyper for short-read sequence data

Genome Biology, 2019
Accurate detection and genotyping of structural variations (SVs) from short-read data is a long-standing area of development in genomics research and clinical sequencing pipelines.
Sai Chen, Peter Krusche, Egor Dolzhenko, Rachel M. Sherman, Roman Petrovski, Felix Schlesinger, Melanie Kirsche, David R. Bentley, Michael C. Schatz, Fritz J. Sedlazeck, Michael A. Eberle   +10 more
doaj   +1 more source

High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

BMC Genomics, 2023
Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik, Mohak Sharda, Lakshminarayanan C P, Kumar Virbhadra, Awadhesh Pandit   +4 more
doaj   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Schroth Gary P, Krüger Detlev H., Kentaro Yoshida, Kahl, Guenter, Kahl, G., Fujibe, Takahiro, Krueger, Detlev H, Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Barrero, Roberto A, Yoshida Kentaro, Luo, S., Barrero Roberto A, Terauchi Ryohei, Albertyn Zayed, Luo, Shujun, Shujun Luo, Kimura, Eiji, Barrero, R.A., Schroth, Gary P., Terauchi, Ryohei, Detlev H Krüger, Yoshida Kentaro, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Yoshida, Kentaro, Kahl Günter, Matsumura, Hideo, Luo Shujun, Krueger Detlev H, Albertyn Zayed, Gary P. Schroth, Kahl Guenter, Matsumura, Hideo, Fujibe Takahiro, Matsumura Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Luo Shujun, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Albertyn, Zayed, Matsumura Hideo, Barrero Roberto A., Schroth, Gary P, Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Kimura Eiji, Roberto A. Barrero, Günter Kahl   +68 more
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BFC: correcting Illumina sequencing errors [PDF]

Bioinformatics, 2015
Abstract Summary: BFC is a free, fast and easy-to-use sequencing error corrector designed for Illumina short reads. It uses a non-greedy algorithm but still maintains a speed comparable to implementations based on greedy methods. In evaluations on real data, BFC appears to correct more errors with fewer overcorrections in comparison to ...
openaire   +2 more sources

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD, Veenendaal, A., Johan T den Dunnen, Martien AM Groenen, Bert W Dibbits, Thomas FC Chin-A-Woeng, Dunnen, J.T., den, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Chin-A-Woeng, T.F.C., Dibbits Bert W, Hindrik HD Kerstens, Richard PMA Crooijmans, Veenendaal Albertine, den Dunnen Johan T, Chin-A-Woeng Thomas FC, Crooijmans, R.P.M.A., Albertine Veenendaal, Groenen Martien AM, Crooijmans Richard PMA, den Dunnen, J.T.   +21 more
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ChIP-seq Analysis in R (CSAR): An R package for the statistical detection of protein-bound genomic regions [PDF]

, 2011
Background In vivo detection of protein-bound genomic regions can be achieved by combining chromatin-immunoprecipitation with next-generation sequencing technology (ChIP-seq). The large amount of sequence data produced by this method needs to be analyzed
Muino, J.M., Angenent, G.C., Krajewski Pawel, Angenent Gerco C, Roeland CHJ van Ham, Pawel Krajewski, Muiño Jose M, Ham, R.C.H.J., van, Kaufmann, K., van Ham Roeland CHJ, Jose M Muiño, Kaufmann Kerstin, van Ham, R.C.H.J., Krajewski, P., Kerstin Kaufmann, Gerco C Angenent   +15 more
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