Results 31 to 40 of about 11,169 (113)

Complete Genome Sequence of Neisseria musculi Using Illumina and PacBio Sequencing

Microbiology Resource Announcements, 2021
Neisseria musculi is an oral commensal of wild-caught mice. Here, we report the complete genome sequence of N. musculi strain NW831, generated using a combination of the Illumina and PacBio platforms.
Eliza Thapa, Jain Aluvathingal, Suvarna Nadendla, Aditya Mehta, Hervé Tettelin, Nathan J. Weyand   +5 more
openaire   +2 more sources

Despite low viral titer in saliva samples, Sanger-based SARS-CoV-2 spike gene sequencing is highly applicable for the variant identification

BMC Medical Genomics, 2023
Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241
Ko Ko, Kazuaki Takahashi, Noriaki Ito, Aya Sugiyama, Shintaro Nagashima, Kei Miwata, Yoshihiro Kitahara, Mafumi Okimoto, Serge Ouoba, Golda Ataa Akuffo, Bunthen E, Tomoyuki Akita, Toshiro Takafuta, Junko Tanaka   +13 more
doaj   +1 more source

Transcriptome sequencing of sesame (Sesamum indicum) using Illumina Platform

The Indian Journal of Agricultural Sciences, 2023
Sesame is an important oil seed crop worldwide and has essential health and medicinal values. In the present study, a high-throughput transcriptome sequencing of sesame was performed using Illumina paired-end sequencing technology for gene and marker ...
P SUPRIYA, A R RAO, K V BHAT
doaj   +1 more source

Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

Genomics Data, 2017
Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj   +1 more source

Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene [PDF]

Scientific Reports, 2016
AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke, Silvie, Verhelst, Judith, Saelens, Xavier   +2 more
openaire   +3 more sources

Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix) [PDF]

PeerJ, 2019
Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to ...
Travis C. Glenn, Todd W. Pierson, Natalia J. Bayona-Vásquez, Troy J. Kieran, Sandra L. Hoffberg, Jesse C. Thomas IV, Daniel E. Lefever, John W. Finger, Bei Gao, Xiaoming Bian, Swarnali Louha, Ramya T. Kolli, Kerin E. Bentley, Julie Rushmore, Kelvin Wong, Timothy I. Shaw, Michael J. Rothrock Jr, Anna M. McKee, Tai L. Guo, Rodney Mauricio, Marirosa Molina, Brian S. Cummings, Lawrence H. Lash, Kun Lu, Gregory S. Gilbert, Stephen P. Hubbell, Brant C. Faircloth   +26 more
doaj   +2 more sources

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]

Nucleic Acids Research, 2015
With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie, Ijaz, Umer Z., D'Amore, Rosalinda, Hall, Neil, Sloan, William T., Quince, Christopher   +5 more
openaire   +4 more sources

Identification of allele-specific KIV-2 repeats and impact on Lp(a) measurements for cardiovascular disease risk

BMC Medical Genomics
The abundance of Lp(a) protein holds significant implications for the risk of cardiovascular disease (CVD), which is directly impacted by the copy number (CN) of KIV-2, a 5.5 kbp sub-region.
Sairam Behera, Jonathan R. Belyeu, Xiao Chen, Luis F. Paulin, Ngoc Quynh H. Nguyen, Emma Newman, Medhat Mahmoud, Vipin K. Menon, Qibin Qi, Parag Joshi, Santica Marcovina, Massimiliano Rossi, Eric Roller, James Han, Vitor Onuchic, Christy L. Avery, Christie M. Ballantyne, Carlos J. Rodriguez, Robert C. Kaplan, Donna M. Muzny, Ginger A. Metcalf, Richard A. Gibbs, Bing Yu, Eric Boerwinkle, Michael A. Eberle, Fritz J. Sedlazeck   +25 more
doaj   +1 more source

A chromosome-level genome assembly of the jade perch (Scortum barcoo)

Scientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu, Ruihan Li, Liqun Xia, Jun Cheng, Hongli Xia, Qiuyao Zhan, Dapeng Yu, Xinxin You, Ruobo Gu, Junmin Xu, Qiong Shi, Chao Bian   +11 more
doaj   +1 more source

A Comparison of Base-calling Algorithms for Illumina Sequencing Technology [PDF]

Briefings in Bioinformatics, 2015
Recent advances in next-generation sequencing technology have yielded increasing cost-effectiveness and higher throughput produced per run, in turn, greatly influencing the analysis of DNA sequences. Among the various sequencing technologies, Illumina is by far the most widely used platform.
Ashley Cacho, Ekaterina Smirnova, Snehalata V. Huzurbazar, Xinping Cui   +3 more
openaire   +2 more sources