Results 31 to 40 of about 1,171,841 (304)
Illumina Sequencing Library Construction from ChIP DNA
The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive.
Wei Zheng
doaj +1 more source
Paragraph: a graph-based structural variant genotyper for short-read sequence data
Accurate detection and genotyping of structural variations (SVs) from short-read data is a long-standing area of development in genomics research and clinical sequencing pipelines.
Sai Chen +10 more
doaj +1 more source
Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik +4 more
doaj +1 more source
Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries
Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200.
Jidong Lang +16 more
doaj +1 more source
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P. +68 more
core +1 more source
Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD +21 more
core +1 more source
BFC: correcting Illumina sequencing errors [PDF]
Abstract Summary: BFC is a free, fast and easy-to-use sequencing error corrector designed for Illumina short reads. It uses a non-greedy algorithm but still maintains a speed comparable to implementations based on greedy methods. In evaluations on real data, BFC appears to correct more errors with fewer overcorrections in comparison to ...
openaire +3 more sources
Background The Illumina sequencing systems demonstrate high efficiency and power and remain the most popular platforms. Platforms with similar throughput and quality profiles but lower costs are under intensive development. In this study, we compared two
Iamshchikov Pavel +6 more
doaj +1 more source
ChIP-seq Analysis in R (CSAR): An R package for the statistical detection of protein-bound genomic regions [PDF]
Background In vivo detection of protein-bound genomic regions can be achieved by combining chromatin-immunoprecipitation with next-generation sequencing technology (ChIP-seq). The large amount of sequence data produced by this method needs to be analyzed
Muino, J.M. +15 more
core +1 more source

