Results 41 to 50 of about 599,955 (287)
Frontiers in Microbiology, 2020 We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria ...
Laura Uelze +12 more
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Trimmomatic: a flexible trimmer for Illumina sequence data [PDF]
Bioinformatics, 2014 Abstract Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance.
Bolger, A. M., Lohse, M., Usadel, Björn
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BMC Medical Genomics, 2023 Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241
Ko Ko +13 more
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A computational method for estimating the PCR duplication rate in DNA and RNA-seq experiments. [PDF]
, 2017 BackgroundPCR amplification is an important step in the preparation of DNA sequencing libraries prior to high-throughput sequencing. PCR amplification introduces redundant reads in the sequence data and estimating the PCR duplication rate is important to
Bansal, Vikas
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Transcriptome sequencing of sesame (Sesamum indicum) using Illumina Platform
The Indian Journal of Agricultural Sciences, 2023 Sesame is an important oil seed crop worldwide and has essential health and medicinal values. In the present study, a high-throughput transcriptome sequencing of sesame was performed using Illumina paired-end sequencing technology for gene and marker ...
P SUPRIYA, A R RAO, K V BHAT
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QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles [PDF]
, 2015 Background: Next generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth ("deep sequencing"), low frequency variants can be detected.
Aerssens, Jeroen +7 more
core +4 more sources
Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification [PDF]
, 2020 Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Barton, Geoffrey J. +8 more
core +3 more sources
Complete Genome Sequence of Neisseria musculi Using Illumina and PacBio Sequencing
Microbiology Resource Announcements, 2021 Neisseria musculi is an oral commensal of wild-caught mice. Here, we report the complete genome sequence of N. musculi strain NW831, generated using a combination of the Illumina and PacBio platforms.
Eliza Thapa +5 more
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Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
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Characterization of pseudorabies virus transcriptome by Illumina sequencing [PDF]
BMC Microbiology, 2015 Pseudorabies virus is a widely-studied model organism of the Herpesviridae family, with a compact genome arrangement of 72 known coding sequences. In order to obtain an up-to-date genetic map of the virus, a combination of RNA-sequencing approaches were applied, as recent advancements in high-throughput sequencing methods have provided a wealth of ...
Oláh, Péter +5 more
openaire +3 more sources