Results 51 to 60 of about 1,171,841 (304)

Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

Genomics Data, 2017
Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj   +1 more source

Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix) [PDF]

PeerJ, 2019
Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to ...
Travis C. Glenn, Todd W. Pierson, Natalia J. Bayona-Vásquez, Troy J. Kieran, Sandra L. Hoffberg, Jesse C. Thomas IV, Daniel E. Lefever, John W. Finger, Bei Gao, Xiaoming Bian, Swarnali Louha, Ramya T. Kolli, Kerin E. Bentley, Julie Rushmore, Kelvin Wong, Timothy I. Shaw, Michael J. Rothrock Jr, Anna M. McKee, Tai L. Guo, Rodney Mauricio, Marirosa Molina, Brian S. Cummings, Lawrence H. Lash, Kun Lu, Gregory S. Gilbert, Stephen P. Hubbell, Brant C. Faircloth   +26 more
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PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +2 more sources

Summary of Illumina sequencing statistics and mapping.

, 2021
Summary of Illumina sequencing statistics and mapping.
J. Sook Chung (9635123), Tsvetan Bachvaroff (3727339), Elena Legrand (11383758), Tracey B. Schock (394735)   +3 more
core   +1 more source

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]

Nucleic Acids Research, 2015
With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie, Ijaz, Umer Z., D'Amore, Rosalinda, Hall, Neil, Sloan, William T., Quince, Christopher   +5 more
openaire   +4 more sources

Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene [PDF]

Scientific Reports, 2016
AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke, Silvie, Verhelst, Judith, Saelens, Xavier   +2 more
openaire   +3 more sources

Routine use of microbial whole genome sequencing in diagnostic and public health microbiology [PDF]

, 2012
This work is partly supported by funding from the Medical Research Council, the European Developing Country Clinical Trials Partnership, the Global Alliance for TB Drug Development, and the European Union.Whole genome sequencing (WGS) promises to be ...
Holden, MTG, Matthew T G Holden, Mark Farrington (148224), Cartwright, EJP, Matthew T. G. Holden (130258), Brown, Nicholas M., Sharon J Peacock, Bentley, SD, Ellington, Matthew J., Claudio U Köser, Parkhill, J, Stephen D. Bentley (137281), Stephen H Gillespie, Stephen D Bentley, Köser, CU, Bentley, Stephen D., Julian Parkhill (30393), Holden, Matthew T. G., Julian Parkhill, Peacock, Sharon J., Edward J. P. Cartwright (148213), Koeser, Claudio U., Peacock, SJ, Stephen H. Gillespie (148218), Edward J P Cartwright, Gordon Dougan, Matthew J. Ellington (148208), Farrington, M, Cartwright, Edward J. P., Dougan, Gordon, Nicholas M Brown, Parkhill, Julian, Mark Farrington, Gordon Dougan (30395), Farrington, Mark, Claudio U. Köser (148202), Gillespie, Stephen H., Matthew J Ellington, Brown, NM, Sharon J. Peacock (35782), Gillespie, SH, Ellington, MJ, Nicholas M. Brown (148222), Dougan, G   +43 more
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A Short Review on Illumina Sequencing Technology

Austin Journal of Veterinary Science & Animal Husbandry, 2022
DNA sequencing is that the method of crucial the supermolecule sequence – the order of nucleotides in deoxyribonucleic acid. It includes any methodology or technology that’s accustomed verify the order of the four bases: A, guanine, cytosine, and T. Illumina technology is sequencing by synthesis approach and is presently the foremost used technology ...
openaire   +1 more source

Identification of allele-specific KIV-2 repeats and impact on Lp(a) measurements for cardiovascular disease risk

BMC Medical Genomics
The abundance of Lp(a) protein holds significant implications for the risk of cardiovascular disease (CVD), which is directly impacted by the copy number (CN) of KIV-2, a 5.5 kbp sub-region.
Sairam Behera, Jonathan R. Belyeu, Xiao Chen, Luis F. Paulin, Ngoc Quynh H. Nguyen, Emma Newman, Medhat Mahmoud, Vipin K. Menon, Qibin Qi, Parag Joshi, Santica Marcovina, Massimiliano Rossi, Eric Roller, James Han, Vitor Onuchic, Christy L. Avery, Christie M. Ballantyne, Carlos J. Rodriguez, Robert C. Kaplan, Donna M. Muzny, Ginger A. Metcalf, Richard A. Gibbs, Bing Yu, Eric Boerwinkle, Michael A. Eberle, Fritz J. Sedlazeck   +25 more
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A chromosome-level genome assembly of the jade perch (Scortum barcoo)

Scientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu, Ruihan Li, Liqun Xia, Jun Cheng, Hongli Xia, Qiuyao Zhan, Dapeng Yu, Xinxin You, Ruobo Gu, Junmin Xu, Qiong Shi, Chao Bian   +11 more
doaj   +1 more source