Results 51 to 60 of about 599,955 (287)
Whole-genome sequencing of Klebsiella pneumoniae isolates to track strain progression in a single patient with recurrent urinary tract infection [PDF]
, 2019 Klebsiella pneumoniae is an important uropathogen that increasingly harbors broad-spectrum antibiotic resistance determinants. Evidence suggests that some same-strain recurrences in women with frequent urinary tract infections (UTIs) may emanate from a ...
Minx, Patrick J +3 more
core +5 more sources
Paired-end sequencing of Fosmid libraries by Illumina [PDF]
Genome Research, 2012 Eliminating the bacterial cloning step has been a major factor in the vastly improved efficiency of massively parallel sequencing approaches. However, this also has made it a technical challenge to produce the modern equivalent of the Fosmid- or BAC-end sequences that were crucial for assembling and analyzing complex genomes during the Sanger-based ...
Williams, Louise J.S. +12 more
openaire +2 more sources
Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]
, 2011 An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin +39 more
core +1 more source
The Personal Genome Project-UK, an open access resource of human multi-omics data [PDF]
, 2019 Integrative analysis of multi-omics data is a powerful approach for gaining functional insights into biological and medical processes. Conducting these multifaceted analyses on human samples is often complicated by the fact that the raw sequencing output
Beck, Stephan +12 more
core +2 more sources
BMC Medical GenomicsThe abundance of Lp(a) protein holds significant implications for the risk of cardiovascular disease (CVD), which is directly impacted by the copy number (CN) of KIV-2, a 5.5 kbp sub-region.
Sairam Behera +25 more
doaj +1 more source
A chromosome-level genome assembly of the jade perch (Scortum barcoo)
Scientific Data, 2022 Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu +11 more
doaj +1 more source
A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200 [PDF]
, 2019 Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads,
Ayine, Monica L. +9 more
core +1 more source
Molecular Oncology, EarlyView.Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra +10 more
wiley +1 more source
Lane-by-Lane Sequencing using Illumina's Genome Analyzer II [PDF]
BioTechniques, 2013 Next-generation sequencing has become an essential tool in molecular biology that has been successfully applied to a broad variety of experimental approaches. While several platforms for next-generation sequencing exist, the most commonly used approach is sequencing-by-synthesis, implemented on Illumina's Genome Analyzer II (GAII) and HiSeq2000 systems.
Michael T. Gravina +2 more
openaire +3 more sources
Molecular Oncology, EarlyView.Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice +16 more
wiley +1 more source