Results 51 to 60 of about 11,169 (113)

Solution-based targeted genomic enrichment for precious DNA samples

BMC Biotechnology, 2012
Background Solution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale.
Shearer Aiden, Hildebrand Michael S, Smith Richard JH   +2 more
doaj   +1 more source

Addressing challenges in the production and analysis of illumina sequencing data [PDF]

BMC Genomics, 2011
Abstract Advances in DNA sequencing technologies have made it possible to generate large amounts of sequence data very rapidly and at substantially lower cost than capillary sequencing. These new technologies have specific characteristics and limitations that require either consideration during project design, or which must be addressed ...
Kircher, M., Heyn, P., Kelso, J. ; https://orcid.org/0000-0002-3618-322X   +2 more
openaire   +4 more sources

Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts [PDF]

Metabarcoding and Metagenomics
Oxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck, Sven Nicolai Katzenmeier, Hans-Werner Breiner, Verena Rubel   +3 more
doaj   +3 more sources

From reads to results: comparing Oxford Nanopore to Illumina sequencing for citrus virus surveillance

BMC Genomics
Background ONT sequencing has been previously evaluated for its ability to detect plant viruses and viroids. Its advantages, such as longer read lengths and real-time analysis, compete with extensively validated Illumina platforms for possible ...
Madelein Dippenaar, Hans Jacob Maree, Rachelle Bester   +2 more
doaj   +1 more source

Illumina-based transcriptome sequencing of mussel Mytilus coruscus mantle

浙江大学学报. 农业与生命科学版, 2015
Mollusk shell is an exoskeleton made up of organic mineral particles and its function is to protect the inner soft body from predators and environmental damage. Due to its superior mechanical properties, such as stiffness, fracture toughness, and tensile
Bao Linfei, Wang Xinxing, He Jianyu, Fan Meihua, Gao Peng, Liao Zhi   +5 more
doaj   +1 more source

Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

npj Science of Food, 2021
Efficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures.
Aoife J. McHugh, Min Yap, Fiona Crispie, Conor Feehily, Colin Hill, Paul D. Cotter   +5 more
doaj   +1 more source

stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.

PLoS ONE
Identifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of limited clinical ...
Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z Metcalfe   +9 more
doaj   +1 more source

Direct comparison of clear DX Nanopore and Illumina sequencing of SARS-CoV-2

Microbiology Spectrum
There are limited data directly comparing SARS-CoV-2 sequencing methods using two major commercial approaches, Oxford Nanopore Technologies Clear DX (ONTDX) and Illumina.
Wesley Hottel, Valerie Reeb, Erik Twait, Kristen Zanon, Munok Hwang, Hosoon Choi, Piyali Chatterjee, Jinhua Xiang, Jeffery Meier, Michael Pentella, Eric McIndoo, Mary Cloud B. Ammons, Chetan Jinadatha, Jack T. Stapleton   +13 more
doaj   +1 more source

Metagenomic data for Halichondria panicea from Illumina and nanopore sequencing and preliminary genome assemblies for the sponge and two microbial symbionts

BMC Research Notes, 2022
Objectives These data were collected to generate a novel reference metagenome for the sponge Halichondria panicea and its microbiome for subsequent differential expression analyses.
Brian W. Strehlow, Astrid Schuster, Warren R. Francis, Donald E. Canfield   +3 more
doaj   +1 more source

Evaluation of de novo assembly technique in the South African abalone Haliotis midae transcriptome: A comparison from Illumina and 454 systems

Genomics Data, 2016
Next generation sequencing platforms have recently been used to rapidly characterize transcriptome sequences from a number of non-model organisms. The present study compares two of the most frequently used platforms, the Roche 454-pyrosequencing and the ...
Barbara Picone, Clint Rhode, Rouvay Roodt-Wilding   +2 more
doaj   +1 more source