Results 1 to 10 of about 487,550 (165)
Improved Protocols for Illumina Sequencing [PDF]
Current Protocols in Human Genetics, 2013 AbstractIn this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 79:18.2.1‐18.2.42.
Iraad F, Bronner +3 more
exaly +4 more sources
Reliability of DNA methylation measures using Illumina methylation BeadChip
Epigenetics, 2021 Illumina BeadChips are widely utilized in epigenome-wide association studies (EWAS). Several studies have reported that many probes on these arrays have poor reliability.
Jack Taylor, Zongli Xu
exaly +2 more sources
Correcting Illumina data [PDF]
Briefings in Bioinformatics, 2014 Next-generation sequencing technologies revolutionized the ways in which genetic information is obtained and have opened the door for many essential applications in biomedical sciences. Hundreds of gigabytes of data are being produced, and all applications are affected by the errors in the data. Many programs have been designed to correct these errors,
Michael Molnar, Lucian Ilie
openaire +2 more sources
An Illumina metabarcoding pipeline for fungi [PDF]
Ecology and Evolution, 2014 AbstractHigh‐throughput metabarcoding studies on fungi and other eukaryotic microorganisms are rapidly becoming more frequent and more complex, requiring researchers to handle ever increasing amounts of raw sequence data. Here, we provide a flexible pipeline for pruning and analyzing fungal barcode (ITS rDNA) data generated as paired‐end reads on ...
Bálint, Miklós +4 more
openaire +3 more sources
Illumina reads correction: evaluation and improvements
Scientific Reports, 2023 Abstract The paper focuses on the correction of Illumina WGS sequencing reads. We provide an extensive evaluation of the existing correctors. To this end, we measure an impact of the correction on variant calling (VC) as well as de novo assembly. It shows, that in selected cases read correction improves the VC results quality. We also
Maciej Długosz, Sebastian Deorowicz
openaire +3 more sources
Nature Communications, 2022 As CRISPR-based therapies enter the clinic, evaluation of safety remains a critical and active area of study. Here the authors use next generation sequencing to achieve high sequencing depth and demonstrate that clinically relevant delivery of high ...
M. Kyle Cromer +11 more
doaj +1 more source
Error rate for imputation from the Illumina BovineSNP50 chip to the Illumina BovineHD chip [PDF]
Genetics Selection Evolution, 2014 Imputation of genotypes from low-density to higher density chips is a cost-effective method to obtain high-density genotypes for many animals, based on genotypes of only a relatively small subset of animals (reference population) on the high-density chip.
Schrooten, Chris +9 more
openaire +7 more sources
Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
BMC Genomics, 2018 Background Transposome-based technologies have enabled the streamlined production of sequencer-ready DNA libraries; however, current methods are highly sensitive to the amount and quality of input nucleic acid.
Stephen Bruinsma +17 more
doaj +1 more source
International Journal of Neonatal Screening, 2023 The collection of dried blood spots (DBS) facilitates newborn screening for a variety of rare, but very serious conditions in healthcare systems around the world.
David J. McBride +13 more
doaj +1 more source
BMC Microbiology, 2023 The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition and functional state between individuals and over time.
Asako Tan +13 more
doaj +1 more source