Results 11 to 20 of about 724,062 (300)

Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]

, 2014
The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
A Adey, A McKenna, BJ Loftus, EL van Dijk, GA Van der Auwera, H Li, H Li, H Li, J Dabney, JD McPherson, Johanna Rhodes, Kirsten Nielsen, L DeFrancesco, M Eisenstein, MA DePristo, MA Quail, Mathew A. Beale, Matthew C. Fisher, R Marine   +18 more
core   +13 more sources

Correcting Illumina data [PDF]

Briefings in Bioinformatics, 2014
Next-generation sequencing technologies revolutionized the ways in which genetic information is obtained and have opened the door for many essential applications in biomedical sciences. Hundreds of gigabytes of data are being produced, and all applications are affected by the errors in the data. Many programs have been designed to correct these errors,
Michael Molnar, Lucian Ilie
openaire   +2 more sources

An Illumina metabarcoding pipeline for fungi [PDF]

Ecology and Evolution, 2014
AbstractHigh‐throughput metabarcoding studies on fungi and other eukaryotic microorganisms are rapidly becoming more frequent and more complex, requiring researchers to handle ever increasing amounts of raw sequence data. Here, we provide a flexible pipeline for pruning and analyzing fungal barcode (ITS rDNA) data generated as paired‐end reads on ...
Bálint, Miklós, Schmidt, Philipp-André, Sharma, Rahul, Thines, Marco, Schmitt, Imke   +4 more
openaire   +3 more sources

Illumina reads correction: evaluation and improvements

Scientific Reports, 2023
Abstract The paper focuses on the correction of Illumina WGS sequencing reads. We provide an extensive evaluation of the existing correctors. To this end, we measure an impact of the correction on variant calling (VC) as well as de novo assembly. It shows, that in selected cases read correction improves the VC results quality. We also
Maciej Długosz, Sebastian Deorowicz
openaire   +3 more sources

Error rate for imputation from the Illumina BovineSNP50 chip to the Illumina BovineHD chip [PDF]

Genetics Selection Evolution, 2014
Imputation of genotypes from low-density to higher density chips is a cost-effective method to obtain high-density genotypes for many animals, based on genotypes of only a relatively small subset of animals (reference population) on the high-density chip.
Schrooten, Chris, Dassonneville, Romain, Ducrocq, Vincent, Brøndum, Rasmus F., Lund, Mogens S., Chen, Jun, Liu, Zengting, González-Recio, Oscar, Pena, Juan, Druet, Tom   +9 more
openaire   +8 more sources

Illumina sequencing of 15 deafness genes using fragmented amplicons [PDF]

, 2014
BACKGROUND: Resequencing of deafness related genes using GS FLX massive parallel sequencing of PCR amplicons spanning selected genes has previously been reported as a successful strategy to discover causal variants.
Coucke, Paul, De Keulenaer, Sarah, De Schrijver, Joachim, Deforce, Dieter, Van Criekinge, Wim, Van Nieuwerburgh, Filip, Vandesompele, Jo   +6 more
core   +1 more source

The Diversity of REcent and Ancient huMan (DREAM): a new microarray for genetic anthropology and genealogy, forensics, and personalized medicine [PDF]

, 2017
The human population displays wide variety in demographic history, ancestry, content of DNA derived from hominins or ancient populations, adaptation, traits, copy number variation (CNVs), drug response, and more. These polymorphisms are of broad interest
Ainan I J Anderson, Dimitrios Arnellos, Eran Elhaik, Gregory Vilshansky, Gunes Ercal, illumina, illumina, illumina, illumina, Leeban Yusuf, Mehdi Pirooznia, Michael L Baird, Teresa Webster, Thermo Fisher Scientific, Thermo Fisher Scientific, Thermo Fisher Scientific, Umberto Esposito, Yontao Lu   +17 more
core   +1 more source

Monitoring Error Rates In Illumina Sequencing [PDF]

Journal of Biomolecular Techniques : JBT, 2016
Guaranteeing high-quality next-generation sequencing data in a rapidly changing environment is an ongoing challenge. The introduction of the Illumina NextSeq 500 and the depreciation of specific metrics from Illumina's Sequencing Analysis Viewer (SAV; Illumina, San Diego, CA, USA) have made it more difficult to determine directly the baseline error ...
Leigh J, Manley, Duanduan, Ma, Stuart S, Levine   +2 more
openaire   +2 more sources

Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1) [PDF]

, 2014
Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72).
Bavegems, Valérie, Broeckx, Bart, Coopman, Frank, De Keulenaer, Sarah, De Meester, Ellen, Deforce, Dieter, Van Nieuwerburgh, Filip, Verhoeven, Geert   +7 more
core   +2 more sources

Simulating Illumina metagenomic data with InSilicoSeq [PDF]

Bioinformatics, 2018
Abstract Motivation The accurate in silico simulation of metagenomic datasets is of great importance for benchmarking bioinformatics tools as well as for experimental design. Users are dependant on large-scale simulation to not only design experiments and new projects but also for accurate estimation ...
Hadrien Gourlé, Oskar Karlsson-Lindsjö, Juliette Hayer, Erik Bongcam-Rudloff   +3 more
openaire   +2 more sources