Results 61 to 70 of about 11,169 (113)

Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

PLOS ONE, 2017
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq.
Chongqing Wen, Liyou Wu, Yujia Qin, Joy D. Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou   +10 more
openaire   +6 more sources

VADER PylT Illumina sequencing data

, 2022
FASTQ files used in data analysis for "Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells."
openaire   +2 more sources

Cross-Platform Evaluation of Established NGS-Based Metabarcoding Methods for Detecting Food Fraud in Pistachio Products

Foods
Next Generation Sequencing is a constantly evolving technology whose applicability is increasingly expanding into the field of routine food analysis.
Sina Rammouz, Jochen Riehle, Ansgar Ferner, Markus Fischer, Christian Schäfers   +4 more
doaj   +1 more source

Historical Demography and Species Distribution Models Shed Light on Speciation in Primates of Northeast India

Ecology and Evolution
Past climate change is one of the important factors influencing primate speciation. Populations of various species could have risen or declined in response to these climatic fluctuations.
Mihir Trivedi, Kunal Arekar, Shivakumara Manu, Lukas F. K. Kuderna, Jeffrey Rogers, Kyle Kai‐How Farh, Tomas Marques Bonet, Govindhaswamy Umapathy   +7 more
doaj   +1 more source

Illumina Sequencing Seminar Series

, 2010
Next week Brent Anderson with Illumina will be hosting a seminar series showcasing presentations from Vanderbilt scientists using Illumina technology to power their next-generation sequencing studies.
openaire   +1 more source

SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data

BMC Bioinformatics, 2010
Background Illumina's second-generation sequencing platform is playing an increasingly prominent role in modern DNA and RNA sequencing efforts. However, rapid, simple, standardized and independent measures of run quality are currently lacking, as are ...
Peterson Daniel A, Cox Murray P, Biggs Patrick J   +2 more
doaj   +1 more source

Promises and pitfalls of Illumina sequencing for HIV resistance genotyping

Virus Research, 2017
Genetic sequencing ("genotyping") plays a critical role in the modern clinical management of HIV infection. This virus evolves rapidly within patients because of its error-prone reverse transcriptase and short generation time. Consequently, HIV variants with mutations that confer resistance to one or more antiretroviral drugs can emerge during sub ...
Chanson J, Brumme, Art F Y, Poon
openaire   +2 more sources

Benchmarking Illumina and Oxford Nanopore Technologies (ONT) sequencing platforms for whole genome sequencing of bacterial genomes and use in clinical microbiology

BMC Medical Genomics
Background In microbial diagnostics, whole-genome sequencing (WGS) is used to address key questions such as species identification, presence of antimicrobial resistance genes (ARGs), virulence genes, and outbreak detection.
Srinithi Purushothaman, Tim Roloff, Adrian Egli, Helena MB Seth-Smith   +3 more
doaj   +1 more source

Characterizing the mouse ES cell transcriptome with Illumina sequencing

Genomics, 2008
Large datasets generated by Illumina sequencing are ideally suited to transcriptome characterization. We generated 3,052,501 27-mer reads from F1 mouse embryonic stem (ES) cell cDNA. Using the ELAND alignment tool, 74.5% of reads matched sequenced mouse resources,
Rosenkranz, R., Borodina, T., Lehrach, H., Himmelbauer, H.   +3 more
openaire   +3 more sources

Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses

Genome Medicine
Background Metagenomics is a powerful approach for the detection of unknown and novel pathogens. Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories.
Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal, Tony Brooks, Cristina Venturini, Charles Miller, Julianne R. Brown, Nathaniel Storey, Laura Atkinson, Timothy Best, Sunando Roy, Sian Goldsworthy, Sergi Castellano, Peter Simmonds, Heli Harvala, Tanya Golubchik, Rachel Williams, Judith Breuer, Sofia Morfopoulou, Oscar Enrique Torres Montaguth   +20 more
doaj   +1 more source